1. Incorporation of lysine from microbial origin into tissue protein of Iberian and Landrace × Large White piglets
- Author
-
M. A. Aguinaga, V. Ruiz-Guerrero, Jose Alberto Conde-Aguilera, J. F. Aguilera, Rosa Nieto, A. I. Navarro, Estación Experimental del Zaidín (EEZ), and Consejo Superior de Investigaciones Científicas [Madrid] (CSIC)
- Subjects
030309 nutrition & dietetics ,animal diseases ,[SDV]Life Sciences [q-bio] ,Lysine ,Tissue protein ,030209 endocrinology & metabolism ,Biology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Animal science ,fluids and secretions ,medicine ,Ammonium ,2. Zero hunger ,chemistry.chemical_classification ,0303 health sciences ,General Veterinary ,Large white ,Small intestine ,Breed ,Amino acid ,medicine.anatomical_structure ,chemistry ,Biochemistry ,Animal Science and Zoology ,Iberian pigs ,Microbial lysine ,15N-lysine ,Pig tissues ,Pig genotypes - Abstract
International audience; The distinct incorporation of lysine from microbial origin in tissues has been investigated in Iberian and Landrace x Large White piglets of 47 +/- 3 d of age (n = 8/genotype) given (15)N-labelled ammonium sulphate added to the diet. Four piglets of each breed were allocated in metabolic cages to prevent coprophagy. All piglets consumed the labelled diet for 10 days and then were slaughtered. Viscera and carcass weights were recorded and gastrointestinal tissues carefully handled. Samples of carcass, liver, and bacterial pellets isolated from the last third of the small intestine, were hydrolyzed, desalted by ion-exchange, and the amino acids eluted analyzed for (15)N-lysine enrichment in a GC-combustion/isotope ratio mass-spectrometer. Background (15)N-lysine in tissues was also determined in 3 piglets per breed. Liver enrichment was considerably higher in Landrace x Large White than in Iberian piglets (0.0273 +/- 0.0013 vs. 0.0167 +/- 0.0013 APE, P
- Published
- 2009
- Full Text
- View/download PDF