Search

Your search keyword '"Leménager, Nicolas"' showing total 6 results
Start Over Author "Leménager, Nicolas" Publisher hal ccsd
6 results on '"Leménager, Nicolas"'

1. Adaptation and correlated fitness responses over two time scales in Drosophila suzukii populations evolving in different environments

Author

Olazcuaga, Laure, Foucaud, Julien, Gautier, Mathieu, Deschamps, Candice, Loiseau, Anne, Leménager, Nicolas, Facon, Benoit, Ravigné, Virginie, Hufbauer, Ruth, Estoup, Arnaud, Rode, Nicolas, Centre de Biologie pour la Gestion des Populations (UMR CBGP), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Colorado State University [Fort Collins] (CSU), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Peuplements végétaux et bioagresseurs en milieu tropical (UMR PVBMT), Institut de Recherche pour le Développement (IRD)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université de La Réunion (UR), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Graduate Degree Program in Ecology, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de La Réunion (UR)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), French Agence National pour la Recherche (ANR project SWING), French programme investissement d'avenir, and the LabEx CEMEB through the AAP 'invited scientist 2016', CeMEB LabEx/University of Montpellier (ANR-10-LABX-0004), INRAE-SPE starting grant, European Regional Development Fund (ERDF), Conseil Regional de La Reunion, European Union program FEFER FSE IEJ 2014-2020 (project CPADROL), INRAE scientific department SPE (AAP-SPE 2016), Occitanie region (France), National Science Foundation (DEB-0949619), USDA Agriculture and Food Research Initiative award (2014-67013-21594), Hatch project 1012868, French Agropolis Fondation (LabEx Agro-Montpellier) through the AAP ' International Mobility' (CfP 2015-02)., ANR-16-CE02-0015,SWING,Invasion mondiale de la drosophile à aile tachetée: Génétique, plasticité et potentiel évolutif(2016), ANR-10-LABX-0004,CeMEB,Mediterranean Center for Environment and Biodiversity(2010), ANR-10-LABX-0001,AGRO,Agricultural Sciences for sustainable Development(2010), and Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro

Subjects
fitness trade‐off, fitness landscape, Facteur écologique, Acclimatization, [SDV]Life Sciences [q-bio], fitness trade-off, Évolution, specialization, reciprocal transplant experiment, Animals, L50 - Physiologie et biochimie animales, experimental evolution, Adaptation physiologique, Adaptation, Ravageur des plantes, Adaptation, Physiological, Research Papers, H10 - Ravageurs des plantes, Culture Media, Physiologie animale, Drosophila, Research Paper
Abstract

The process of local adaptation involves differential changes in fitness over time across different environments. Although experimental evolution studies have extensively tested for patterns of local adaptation at a single time point, there is relatively little research that examines fitness more than once during the time course of adaptation. We allowed replicate populations of the fruit pest Drosophila suzukii to evolve in one of eight different fruit media. After five generations, populations with the highest initial levels of maladaptation had mostly gone extinct, whereas experimental populations evolving on cherry, strawberry and cranberry media had survived. We measured the fitness of each surviving population in each of the three fruit media after five and after 26 generations of evolution. After five generations, adaptation to each medium was associated with increased fitness in the two other media. This was also true after 26 generations, except when populations that evolved on cranberry medium developed on cherry medium. These results suggest that, in the theoretical framework of a fitness landscape, the fitness optima of cherry and cranberry media are the furthest apart. Our results show that studying how fitness changes across several environments and across multiple generations provides insights into the dynamics of local adaptation that would not be evident if fitness were analysed at a single point in time. By allowing a qualitative mapping of an experimental fitness landscape, our approach will improve our understanding of the ecological factors that drive the evolution of local adaptation in D. suzukii., Using fitness landscape theory to study adaptation and correlated fitness responses over two time scales in Drosophila suzukii populations evolving in different fruit environments.

Published
2021
Full Text
View/download PDF

2. Integrative taxonomy confirms that Gregarina garnhami and G. acridiorum (Apicomplexa, Gregarinidae), parasites of Schistocerca gregaria and Locusta migratoria (Insecta, Orthoptera), are distinct species

Author

Florent, Isabelle, Chapuis, Marie Pierre, Labat, Amandine, Boisard, Julie, Leménager, Nicolas, Michel, Bruno, Desportes-Livage, Isabelle, Molécules de Communication et Adaptation des Micro-organismes (MCAM), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), Centre de Biologie pour la Gestion des Populations (UMR CBGP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Structure et Instabilité des Génomes (STRING), Muséum national d'Histoire naturelle (MNHN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), This work was supported by a grant from the French Agence Nationale de la Recherche [LabEx ANR-10-LABX-0003-BCDiv], in the program 'Investissements d’avenir' [ANR-11-IDEX-0004-02], by several interdisciplinary Programs of the MNHN (ATM-Microorganismes, ATM-Génomique et Collections, ATM-Emergence, AVIV department), the CNRS (Julie Boisard’s PhD fellowship, 2018–2021) and the French Agricultural Research Centre for International Development (CIRAD)., and ANR-11-IDEX-0004,SUPER,Sorbonne Universités à Paris pour l'Enseignement et la Recherche(2011)

Subjects
Gregarines, [SDV]Life Sciences [q-bio], Species delimitation, Orthoptera, SSU rDNA phylogeny, Phenotypic plasticity, Biodiversity
Abstract

International audience; Orthoptera are infected by about 60 species of gregarines assigned to the genus Gregarina Dufour, 1828. Among these species, Gregarina garnhami Canning, 1956 from Schistocerca gregaria (Forsskål, 1775) was considered by Lipa et al. in 1996 to be synonymous with Gregarina acridiorum (Léger 1893), a parasite of several orthopteran species including Locusta migratoria (Linné, 1758). Here, a morphological study and molecular analyses of the SSU rDNA marker demonstrate that specimens of S. gregaria and specimens of L. migratoria are infected by two distinct Gregarina species, G. garnhami and G. acridiorum, respectively. Validation of the species confirms that molecular analyses provide useful taxonomical information. Phenotypic plasticity was clearly observed in the case of G. garnhami: the morphology of its trophozoites, gamonts and syzygies varied according to the geographical location of S. gregaria and the subspecies infected.; Les orthoptères sont parasités par environ soixante espèces de grégarines affiliées au genre Gregarina (Dufour, 1828). Parmi ces espèces Gregarina garnhami (Canning, 1956), décrite chez Schistocerca gregaria (Forskål,1775), a été mise en synonymie par Lipa et al. en 1996 avec Gregarina acridiorum (Léger 1893), parasite de plusieurs espèces d’orthoptères dont Locusta migratoria (Linné, 1758). Ici, une étude morphologique et des analyses moléculaires du marqueur SSU rDNA démontrent que les spécimens de S. gregariaet ceux de L. migratorias ont infectés par 2 espèces distinctes de grégarines, Gregarina garnhami et Gregarina acridiorum, respectivement. La validation de ces espèces confirme l’importance des informations fournies par les analyses moléculaires dans les études taxonomiques. Une plasticité phénotypique a été clairement observée dans le cas de G. garnhami :la morphologie de ses trophozoïtes, gamontes et syzygies varie selon la localisation géographique et la sous-espèce de S. gregaria infectée.

Published
2021
Full Text
View/download PDF

3. Integrative systematic revision reveals cryptic species in the Calliptamus genus

Author

Chapuis, MARIE-PIERRE, Benoit, Laure, Foucart, Antoine, Dehne Garcia, Alexandre, Rasplus, Jean Yves, Leménager, Nicolas, Michel, Bruno, Coeur D'Acier, Armelle, ProdInra, Migration, Centre de Biologie pour la Gestion des Populations (UMR CBGP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
Calliptamus, cryptic species, [SDV.BA] Life Sciences [q-bio]/Animal biology, [SDV.BA]Life Sciences [q-bio]/Animal biology, morphology, glaciation, haplotype network, ComputingMilieux_MISCELLANEOUS, RAD-Seq
Abstract

The systematics of the genus Calliptamus mainly relies on the shape of the wing and phallic complex, and on color pattern of hind femora of males only. However, geographical variation in these morphological characters hinders species determination. These difficulties may explain why the species delimitation within the genus Calliptamus has been controversial, with numerous confusions, descriptions, synonymies and subspecies. The purpose of this research is to provide a taxonomic review of the genus Calliptamus, based on an integrative approach including morphological, molecular and biological data. Beyond laying the foundations for a revised classification, this project will unravel the historical biogeography of the genus. The Orthoptera Species File currently counts twelve species whose type depositories are known for the Palaearctic genus Calliptamus. We collected with success 9 species, represented by an average of 10 (from 1 to 25) individuals and 6 (from 1 to 17) localities. Since a phylogeny based on a few nuclear genes only may fail to resolve the basal relationships, we used the method of Restrictionsite- associated DNA sequencing (RAD-seq), which showed promises to infer shallow relationships in insects. We showed a success of the method, despite the huge size for the genomes of Calliptamus sp., which we estimated to be ~7.65- 8.39 Gb. We confirmed the two clades defined on the basis of genitalia shape: a Northern group includes species from the cold and wet temperate regions of Eurasia (e.g. C. italicus) and a southern group includes species from the warm and dry mediterranean region (e.g. c. barbarus). we interpreted diversification within these two clades as the result of biogeographic factors, such as isolation in high mountain ranges and islands. We discovered that the species C. barbarus was not monophyletic and we used finer-scale sampling and the COI barcode to understand further its evolutionary history. Morphological analyses of numerous characters, including the hind wing, hind femur, shape of the pronotum and internal and external structures of the male genitalia, are in process to supplement genetic analyses.

Published
2019

4. Investigating the potential specialization of the pest Drosophila suzukii to different host fruits

Author

Olazcuaga, Laure, Foucaud, Julien, Rode, Nicolas, Facon, Benoit, Ausset, Aurélien, Leménager, Nicolas, Loiseau, Anne, Ravigné, Virginie, Gautier, Mathieu, Estoup, Arnaud, HUFBAUER, Ruth, Centre de Biologie pour la Gestion des Populations (UMR CBGP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Peuplements végétaux et bioagresseurs en milieu tropical (UMR PVBMT), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université de La Réunion (UR), Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Colorado State University [Fort Collins] (CSU), European Society of Evolutionnary Biology (ESEB). INT., and Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)

Subjects
Animal biology, [SDV.BA]Life Sciences [q-bio]/Animal biology, Biologie animale, fungi, food and beverages, drosophila
Abstract

National audience; Theory predicts that adaptive trade-offs across environments promote the evolution of specialized genotypes. However, generalist genotypes that are not better adapted to a specific environment are often observed in nature. Generalists can be maintained despite trade-offs between environments under two circumstances: when environmental conditions are variable spatially or temporally or when there is no genetic variability to evolve toward specialized genotypes. Drosophila suzukii is an insect pest that attacks the fruits of a wide range of host plants. The availability of each type of host is restricted to a certain time of the year, which might prevent the evolution of specialist genotypes. We can hypothesize that if flies were able to develop on the same host for multiple generations, increased specialization should arise. We tested this hypothesis using an experimental evolution approach. We maintained replicate populations of flies in three different fruit environments. After five generations of experimental selection, each population evolved increased fitness on the fruit it was maintained on as well as on the two other fruits. In addition, preference tests showed that lines did not prefer their own fruit environment. These results indicate that the populations adapted to laboratory conditions, and that there was no response to potential trade-offs across environments. This suggests, that such trade-offs are weak or that there is no genetic variation for specialization to evolve. To confirm this finding, we are maintaining the experimental lines for another five additional generations. Our preliminary results highlight the difficulty of studying the evolution of specialization.

Published
2018

5. lnvestigating the potential specialization of the pest Drosophila suzukii to different host fruits

Author

Olazcuaga, L., Foucaud, Julien, Rode, N., Ausset, Aurélien, Leménager, Nicolas, Loiseau, Anne, Hurbauer, R.A., Facon, Benoît, Ravigné, Virginie, Gautier, Mathieu, Estoup, Arnaud, Centre de Biologie pour la Gestion des Populations (UMR CBGP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Colorado State University [Fort Collins] (CSU), Peuplements végétaux et bioagresseurs en milieu tropical (UMR PVBMT), and Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université de La Réunion (UR)

Subjects
fungi, food and beverages, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Abstract

National audience; Theory predicts that adaptive trade-offs across environments promote the evolution of specialized genotypes. However, generalist genotypes that are not better adapted to a specific environment are often observed in nature. Generalists can be maintained despite trade-offs between environments under two circumstances: when environmental conditions are variable spatially or temporally or when there is no genetic variability to evolve toward specialized genotypes. Drosophila suzukii is an insect pest that attacks the fruits of a wide range of host plants. The availability of each type of hast is restricted to a certain time of the year, which might prevent the evolution of specialist genotypes. We can hypothesize that if flies were able to develop on the same hast for multiple generations, increased specialization should arise. We tested this hypothesis using an experimental evolution approach. We maintained replicate populations of flies in three different fruit environments. After five generations of experimental selection, each population evolved increased fitness on the fruit it was maintained on as well as on the two other fruits. In addition, preference tests showed that lines did not prefer their own fruit environment. These results indicate that the populations adapted to laboratory conditions, and that there was no response to potential trade-offs across environments. This suggests, that such trade-offs are weak or that there is no genetic variation for specialization to evolve. To confirm this finding, we are maintaining the experimental lines for another five additional generations. Our preliminary results highlight the difficulty of studying the evolution of specialization.

Published
2018

6. The termiticidal activity of Sextonia rubra (Mez) van der Werff (Lauraceae) extract and its active constituent rubrynolide

Author

Rodrigues, Alice M S, Amusant, Nadine, Beauchêne, Jacques, Eparvier, Véronique, Leménager, Nicolas, Baudassé, Christine, Espindola, Laila S, Stien, Didier, Centre National de la Recherche Scientifique (CNRS), Institut de Chimie des Substances Naturelles (ICSN), and Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)

Subjects
K50 - Technologie des produits forestiers, Insecticides, Nasutitermes, Magnetic Resonance Spectroscopy, Insecticide d'origine végétale, F60 - Physiologie et biochimie végétale, Isoptera, Alkenes, Lauraceae, Reticulitermes flavipes, Bois, Acetals, Animals, J12 - Manutention, transport, stockage et conservation des produits forestiers, [CHIM]Chemical Sciences, Bois tropical, ComputingMilieux_MISCELLANEOUS, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Plant Extracts, Wood, French Guiana, Ocotea, Préservation du bois
Abstract

International audience; BACKGROUND: Termites are degradation agents that inflict severe damage on wood. Some long-lasting Amazonian trees can resist these insects by producing toxic secondary metabolites. These metabolites could potentially replace synthetic termiticidal products which are becoming more restricted to use. RESULTS: Sextonia rubra is resistant to termite-induced degradation. It has been demonstrated that this species naturally produces an ethyl-acetate-soluble termiticidal metabolite, rubrynolide, to protect its wood. Assays in the presence of tropical and invasive termites established that both rubrynolide and crude ethyl acetate extract from S. rubra wood can be used as a treatment for the protection of sensitive woods against termites. CONCLUSION: Rubrynolide and S. rubra extract are promising candidates for the replacement of synthetic termiticides.

Published
2011
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results