1. Sensitive and specific detection of Ewing sarcoma minimal residual disease in ovarian and testicular tissues
- Author
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Laure, Chaput, Grèze, Victoria, Pascale, Halle, Nina, Robin, Bruno, Pereira, Lauren, Véronèse, Hervé, Lejeune, Philippe, Durand, Guillaume, Martin, Sanfilippo, Sandra, Michel, Canis, Justyna, Kanold, Andrei, Tchirkov, Brugnon, Florence, Imagerie Moléculaire et Stratégies Théranostiques (IMoST), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), CHU Estaing [Clermont-Ferrand], CHU Clermont-Ferrand, Unité de Biostatistiques [CHU Clermont-Ferrand], Direction de la recherche clinique et de l’innovation [CHU Clermont-Ferrand] (DRCI), and CHU Clermont-Ferrand-CHU Clermont-Ferrand
- Subjects
[SDV]Life Sciences [q-bio] - Abstract
International audience; Sensitive and specific detection of Ewing sarcoma minimal residual disease in ovarian and testicular tissues Ewing sarcoma (EWS) is a common pediatric solid tumor with high metastatic potential. Due to toxic effects of treatments on reproductive functions, the cryopreservation of ovarian tissue (OT) or testicular tissue (TT) is recommended to preserve fertility. However, the risk to reintroduce residual metastatic tumor cells should be evaluated before fertility restoration. Our goal was to validate a sensitive and specific approach for EWS minimal residual disease (MRD) detection in frozen germinal tissues. Thawed OT (n=12) and TT (n=14) were contaminated with tumor RD-ES cells (10, 100 and 1000 cells) and EWS-FLI1 tumor-specific transcript was quantified with RT-qPCR. All contaminated samples were found to be positive, with the strong correlation between RD-ES cell numbers and EWS-FLI1 levels in OT (r=0.93) and TT (r=0.96) (P
- Published
- 2020