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Leptospirosis, an environmental infectious disease of bacterial origin, is the infectious disease with the highest associated mortality in Seychelles. In small island territories, the occurrence of the disease is spatially heterogeneous and a better understanding of the environmental factors that contribute to the presence of the bacteria would help implement targeted control. The present study aimed at identifying the main environmental parameters correlated with animal reservoirs distribution and Leptospira infection in order to delineate habitats with highest prevalence. We used a previously published dataset produced from a large collection of rodents trapped during the dry and wet seasons in most habitats of Mah&eacute, the main island of Seychelles. A land use/land cover analysis was realized in order to describe the various environments using SPOT-5 images by remote sensing (object-based image analysis). At each sampling site, landscape indices were calculated and combined with other geographical parameters together with rainfall records to be used in a multivariate statistical analysis. Several environmental factors were found to be associated with the carriage of leptospires in Rattus rattus and Rattus norvegicus, namely low elevations, fragmented landscapes, the proximity of urbanized areas, an increased distance from forests and, above all, increased precipitation in the three months preceding trapping. The analysis indicated that Leptospira renal carriage could be predicted using the species identification and a description of landscape fragmentation and rainfall, with infection prevalence being positively correlated with these two environmental variables. This model may help decision makers in implementing policies affecting urban landscapes and/or in balancing conservation efforts when designing pest control strategies that should also aim at reducing human contact with Leptospira-laden rats while limiting their impact on the autochthonous fauna.

Background Leptospirosis is a bacterial zoonosis caused by pathogenic Leptospira for which rats are considered as the main reservoir. Disease incidence is higher in tropical countries, especially in insular ecosystems. Our objectives were to determine the current burden of leptospirosis in Seychelles, a country ranking first worldwide according to historical data, to establish epidemiological links between animal reservoirs and human disease, and to identify drivers of transmission. Methods A total of 223 patients with acute febrile symptoms of unknown origin were enrolled in a 12-months prospective study and tested for leptospirosis through real-time PCR, IgM ELISA and MAT. In addition, 739 rats trapped throughout the main island were investigated for Leptospira renal carriage. All molecularly confirmed positive samples were further genotyped. Results A total of 51 patients fulfilled the biological criteria of acute leptospirosis, corresponding to an annual incidence of 54.6 (95% CI 40.7–71.8) per 100,000 inhabitants. Leptospira carriage in Rattus spp. was overall low (7.7%) but dramatically higher in Rattus norvegicus (52.9%) than in Rattus rattus (4.4%). Leptospira interrogans was the only detected species in both humans and rats, and was represented by three distinct Sequence Types (STs). Two were novel STs identified in two thirds of acute human cases while noteworthily absent from rats. Conclusions This study shows that human leptospirosis still represents a heavy disease burden in Seychelles. Genotype data suggests that rats are actually not the main reservoir for human disease. We highlight a rather limited efficacy of preventive measures so far implemented in Seychelles. This could result from ineffective control measures of excreting animal populations, possibly due to a misidentification of the main contaminating reservoir(s). Altogether, presented data stimulate the exploration of alternative reservoir animal hosts., Author summary Leptospirosis is an emerging environmental infectious disease caused by corkscrew shaped bacteria called Leptospira. Humans usually get infected during recreational or work-related outdoor activities through contact with urine excreted by animal reservoirs. As a zoonotic disease, leptospirosis is a good example of the One Health concept for it links humans, animals and ecosystems in a web of pathogen maintenance and transmission. This zoonosis is highly prevalent in the tropics and especially in tropical islands. Seychelles archipelago has been reported as the country with highest human incidence worldwide, although figures are based on dated studies and/or poorly specific tests. The presented investigation aimed at providing an updated information on human leptospirosis burden in Seychelles and exploring the transmission chains in their environmental aspects. Presented data confirms that the disease still heavily impacts the country. Genotyping of pathogenic Leptospira in human acute cases reveals that three distinct Sequence Types (STs) are involved in the disease. However, rats typically considered as the main reservoir in Seychelles, harbor only one of these STs, found only in a minority of human cases. Hence, it appears that rats are likely not the main reservoir of leptospirosis in Seychelles, which has important consequences in terms of preventive measures to be implemented for a better control of human leptospirosis.

International audience; Seabird movements during foraging trips and their preference for particular areas have recently been the focus of many studies aimed at gaining a better understanding of the ecological requirements of several species. During the last decade, the use of new devices, such as Global Positioning System (GPS) devices and geo-locator loggers, has allowed researchers to perform more investigations of this type. GPS devices were used on Wedge-tailed Shearwaters (Puffinus pacificus) breeding on Aride Island, Seychelles, to identify the main foraging areas used during early chick-rearing and to assess at-sea foraging habitat selection. Thirteen foraging trips were recorded, 61.5% of which lasted 1 day. One main foraging area, located approximately 100 km east of the colony just outside a granitic bank characterized by upwelling and higher values of primary production compared to surrounding areas, was identified. The foraging area size (3,313 km2) was much smaller than that identified during late chick-rearing (160,000 km2) in a previous study. This is probably due to the exigency to feed chicks more regularly and hence to find foraging areas closer to the colony during the early chick-rearing. The identification of key marine conservation areas, like those identified in this study, is a priority for designating marine Important Bird Areas and identifying habitat management measures. The results of this study should be relevant for the development of conservation plans for Wedge-tailed Shearwaters and for other seabirds in the area.

The procellariiformes are one of the most endangered orders in the world. In addition, information on population estimates, trends, movements at sea and population dynamic is lacking for many species. We analysed almost the full life cycle of two sympatric species: the tropical (Puffinus bailloni, TS) and the wedge-tailed shearwater (P. pacificus, WS) breeding at Aride Island (Seychelles), in order to understand the interactions between them and the environment both at sea and in the colony. We assessed habitat selection, abundance, distribution and breeding success of the two species at the colony and the habitat selection and distribution at sea. The population was 15,000 and 30,000 pairs for WS and TS respectively; the TS is possibly in decline. Both species selected steep and lowly vegetated areas; the WS also selected boulder-like zones. The TS nests year-round but it has a peak of breeding at the end of the WS breeding season and its failure rate is higher when the two species co-exist. We found spatial, rather than ecological, segregation between the two species at sea in both foraging behaviour (diving depth) and inter-breeding areas. On land they are likely to compete for breeding sites with the WS often over competing the TS. We then suggest future conservation plans focused on both species but especially on the TS., Cette étude analyse la quasi-totalité du cycle de vie de deux espèces de puffins sympatriques, le puffin tropical (Puffinus bailloni, PT) et le puffin du Pacific (P. pacificus, PP) nichant sur Aride Island (Seychelles). L’objectif est de comprendre les interactions existantes entre ces espèces, ainsi que l’influence de l’environnement, au sein de la colonie et dans le milieu marin. Le nombre de couples ont était estime à 15,000 pour le PP et 30,000 pour le PT (où la population est probablement en déclin). Ils sélectionnent davantage des zones escarpées avec des végétations basses. Les PP montrent également une préférence pour les zones rocheuses avec un sol profond. Les PT nichent pendant toute l’année mais de manière plus intensive en fin de saison de reproduction des PP et le taux d'échec de la reproduction est maximal durant la période où les deux espèces nichent en même temps. Dans le milieu marin, il-y-a une ségrégation spatiale entre les deux espèces, exploitant des zones d’alimentation bien distinctes hors saison de reproduction et se distinguant également dans leur comportement alimentaire (profondeur de plonge). Dans la colonie les deux espèces sont en concurrence sur le site de reproduction mais les PP semblent être de meilleurs compétiteurs. Nous proposons la mise en place de mesures de conservation pour les deux espèces, mais favorisant spécialement les PT.