Author: "Florence Fontaine" / Publisher: hal ccsd - Searchworks@Jio Institute Digital Library Search Results

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51 results on '"Florence Fontaine"'

1. L’holobionte vigne, un levier biologique pour lutter contre les dépérissements ?

Author: Pauline Bettenfeld, Sophie TROUVELOT, Olivier Fernandez, Florence Fontaine, Pierre-Emmanuel Courty, Agroécologie [Dijon], Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), and EL Mjiyad, Noureddine
Subjects: [SDE] Environmental Sciences, microbiote, [SDE]Environmental Sciences, maladies du bois de la vigne, holobionte, physiologie de la vigne
Abstract: National audience; Le concept d’organisme pluricellulaire isolé montre actuellement ses limites. En effet, unorganisme interagit avec différentes communautés microbiennes (constituant son microbiote)qui modulent ses propriétés, telles que sa physiologie (ex. réponse à une attaque pathogène).L’association hôte-microorganismes définit un holobionte, dont le fonctionnement est influencépar la dynamique spatio-temporelle de leurs interactions. Ainsi, un dysfonctionnement del’holobionte vigne (par exemple lié à des pratiques culturales inadaptées ou aux changementsclimatiques) pourrait entraîner le développement de maladies ou de désordres physiologiques,sources de dépérissements, de la même manière que l’altération du microbiote observée danscertaines maladies humaines (ex. maladies coeliaques). L’analyse des communautés demicroorganismes associées à un végétal a été jusque récemment limitée, car la plupart desmicroorganismes n’est pas isolable et cultivable in vitro. De nouvelles techniques (nommées -omics) permettent dorénavant de définir les caractéristiques taxonomiques et fonctionnelles (ex.génome, métabolome et protéome) du microbiote total (bactéries/champignons cultivables ounon) associé à une plante. Dans le cadre du projet Holoviti, nous cherchons à caractériser lerôle des microbiotes dans l’homéostasie de la vigne en comparant l’holobionte de ceps sains etaffectés par un dépérissement (Esca, court-noué ou lié au 161-49C). L’un de nos objectifs estde définir des bio-indicateurs taxonomiques et fonctionnels (végétaux et microbiens) de l’étatsanitaire de l’hôte. À terme, la « manipulation » de la composante microbienne de l’holobiontepourrait permettre d’améliorer les services écosystémiques qu’il remplit, comme la résistanceà des agents pathogènes ou à des stress abiotiques.
Published: 2021

2. In planta Activity of Novel Copper(II)-Based Formulations to Inhibit the Esca-Associated Fungus Phaeoacremonium minimum in Grapevine Propagation Material

Author: Christophe Clément, Laura Mugnai, Andrea Simoni, Florence Fontaine, Livio Antonielli, Stéphane Compant, Vincenzo Mondello, Stefano Di Marco, Enrico Battiston, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0106 biological sciences, [SDV]Life Sciences [q-bio], Context (language use), formulation, Fungus, Plant Science, lcsh:Plant culture, 01 natural sciences, 03 medical and health sciences, Cutting, grapevine, wood diseases, colonization, plant defense, Plant defense against herbivory, fungicide, lcsh:SB1-1110, nursery, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Original Research, 2. Zero hunger, 0303 health sciences, biology, Chemistry, Inoculation, hydroxyapatite, Pesticide, biology.organism_classification, colonization, Fungicide, Horticulture, Rootstock, 010606 plant biology & botany
Abstract: Grapevine trunk diseases (GTDs) are a serious and growing threat to vineyards worldwide. The need for innovative control tools persists since pesticides used against some GTDs have been banned and only methods to prevent infections or to reduce foliar symptoms have been developed so far. In this context, the application of imaging methods, already applied to study plant–microbe interactions, represents an interesting approach to understand the effect of experimental treatments applied to reduce fungal colonization, on GTD-related pathogens activity. To this aim, trials were carried out to evaluate the efficacy of copper-based treatments, formulated with hydroxyapatite (HA) as co-adjuvant with innovative delivery properties, loaded with two different copper(II) compounds (tribasic sulfate and sulfate pentahydrate), and applied to grapevine propagation material to inhibit fungal wood colonization. The treated rootstock (Vitis berlandieri × Vitis riparia cv. K5BB) and scion cuttings (Vitis vinifera L., cv. Chardonnay) had been inoculated with a strain of Phaeoacremonium minimum (Pmi) compared to uninoculated rootstocks. Experimental treatments were applied during the water-soaking process, comparing the copper(II) compounds pure or formulated with HA, to hydrate the cuttings. After callusing, grafted vines were grown under greenhouse conditions in a nursery and inoculated with Pmi::gfp7 or with Pmi wild-type. Fifteen weeks post-inoculation, woody tissues close to the inoculation site were sampled to evaluate the efficiency of the treatments by studying the plant–microbe interaction by confocal laser scanning microscopy (CLSM). Copper and further elements were also quantified in the same tissues immediately after the treatments and on the CLSM samples. Finally, the grapevine defense responses were studied in the leaves of cuttings treated with the same formulations. The present investigation confirmed the relevant interaction of Pmi and the related transformed strain on the vascular tissues of grafted vines. Furthermore, in vitro assay revealed (i) the fungistatic effect of HA and the reduced effect of Cu fungicide when combined with HA. In planta assays showed (ii) the reduction of Pmi infection in propagation material treated with HA-Cu formulations, (iii) the movement of HA-Cu formulations inside the plant tissues and their persistence over time, and (iv) the plant defense reaction following the treatment with pure HA or Cu, or combined.
Published: 2021

3. Sap Flow Disruption in Grapevine Is the Early Signal Predicting the Structural, Functional, and Genetic Responses to Esca Disease

Author: Loris Ouadi, Emilie Bruez, Sylvie Bastien, Amira Yacoub, Cindy Coppin, Lucia Guérin-Dubrana, Florence Fontaine, Jean-Christophe Domec, Patrice Rey, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Santé et agroécologie du vignoble (UMR SAVE), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de Recherche Oenologie [Villenave d'Ornon], Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut des Sciences de la Vigne et du Vin (ISVV), Interactions Sol Plante Atmosphère (UMR ISPA), Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ANR-17-ASIE-0007,CWSSEA,Assessments of vulnerability of mature and secondary forests to climatic water stress in Southeast Asia(2017), and ANR-18-PRIM-0006,SWATCH,Effet du changement climatique sur les stratégies d'amélioration de l'utilisation en eau des bassin versants et des systèmes agrosylvopastoraux Méditerranéens(2018)
Subjects: 0106 biological sciences, 0301 basic medicine, Stomatal conductance, stomatal activity, [SDV]Life Sciences [q-bio], Esca, Growing season, Plant Science, Disease, Biology, 01 natural sciences, Vineyard, SB1-1110, 03 medical and health sciences, sap flow, ComputingMilieux_MISCELLANEOUS, Original Research, Transpiration, 2. Zero hunger, Flow disruption, fungi, Plant culture, food and beverages, grapevine, Horticulture, 030104 developmental biology, Vascular network, [SDE]Environmental Sciences, gene expression, 010606 plant biology & botany
Abstract: International audience; Fungal species involved in Esca cause the formation of grapevine wood necroses. It results in the deterioration of vascular network transport capacity and the disturbance of the physiological processes, leading to gradual or sudden grapevine death. Herein, for two consecutive growing seasons, a detailed analysis of the structural (wood necrosis and leaf discoloration) and physiological parameters related to the water use of healthy and esca-symptomatic grapevines was conducted. Measurements were carried out on 17-year-old grapevines that expressed, or not, Esca-leaf symptoms in a vineyard of the Bordeaux region (France). Whole-plant transpiration was recorded continuously from pre-veraison to harvest, using noninvasive sap flow sensors. Whole-plant transpiration was systematically about 40–50% lower in Esca-diseased grapevines compared with controls, and this difference can be observed around 2 weeks before the first Esca-foliar symptoms appeared in the vineyard. Unlike grapevine sap flow disruption, structural (e.g., leaf discolorations), functional (e.g., stomatal conductance, photosynthetic activity, phenolic compounds), and genetic (e.g., expression of leaf-targeted genes) plant responses were only significantly impacted by Esca at the onset and during leaf symptoms development. We conclude that sap flow dynamic, which was related to a high level of a white-rot necrosis, provides a useful tool to predict plant disorders due to Esca-grapevine disease.
Published: 2021

4. Current knowledge on Grapevine Trunk Diseases with complex etiology: a systemic approach

Author: Florence Fontaine, Marion Claverie, Martin Notaro, Jacques Wery, Institut Français de la Vigne et du Vin (IFV), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Fonctionnement et conduite des systèmes de culture tropicaux et méditerranéens (UMR SYSTEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre International de Hautes Etudes Agronomiques Méditerranéennes - Institut Agronomique Méditerranéen de Montpellier (CIHEAM-IAMM), Centre International de Hautes Études Agronomiques Méditerranéennes (CIHEAM)-Centre International de Hautes Études Agronomiques Méditerranéennes (CIHEAM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and The authors also acknowledge the V1303 CASDAR project and its leader C. Chevrier for supporting this study, and the CASDAR and the Ministère de l’Alimentation, de l’Agriculture et de la Forêt for funding.
Subjects: [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, medicine.medical_specialty, conceptual model, [SDV]Life Sciences [q-bio], Esca, Plant Science, Horticulture, Biology, 03 medical and health sciences, lcsh:Botany, medicine, Systemic approach, Intensive care medicine, Botryosphaeria dieback, impacts, 030304 developmental biology, 2. Zero hunger, 0303 health sciences, 030306 microbiology, foliar symptoms, food and beverages, Trunk, lcsh:QK1-989, hypotheses, conceptual model, hypotheses, Etiology, Current (fluid), Agronomy and Crop Science
Abstract: International audience; Among all causes of grapevine decline, Grapevine Trunk Diseases (GTDs) are major concerns for grape growers. This paper reviews knowledge and proposes hypotheses on two major GTDs, esca and Botryosphaeria dieback, and assembles a conceptual model. The objective was to collect information into a sequence, from grapevine nursery propagation processes, through foliar symptom expression, to plant death in mature vineyards. Pathogen infection and colonization steps in woody vine tissues, and the hypotheses that have been formulated to explain the outburst of foliar symptoms, are reported and discussed. Factors that could aggravate or repress GTD symptoms and incidence expansion are also addressed. Vine physiology and pathology together could expand understanding of these diseases. Knowledge and hypotheses that need validation are summarized, and a conceptual model is proposed to explain the occurrence of symptoms and the influencing factors. The model could be useful to cope with the complexity of GTDs, and as a starting point for research to unravel knowledge gaps and suggest new disease management strategies.
Published: 2020

5. Sodium arsenite effect on Vitis vinifera L. Physiology

Author: Julie Vallet, Aurélie Songy, Christophe Clément, Marie Gantet, Mary-Lorène Goddard, Céline Tarnus, Philippe Larignon, Anne Boos, Pascale Ronot, Florence Fontaine, SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Grand Lyon, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Institut Pluridisciplinaire Hubert Curien (IPHC), Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Sciences Analytiques et Interactions Ioniques et Biomoléculaires (DSA-IPHC), Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de chimie moléculaire (LCM), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Unité de Recherche Vigne et Vins de Champagne Stress et Environnement - EA 4707 (URVVC), Centre Technique Interprofessionnel de la Vigne et du Vin (ITV), Laboratoire d'innovation moléculaire et applications (LIMA), Centre National de la Recherche Scientifique (CNRS)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Université de Strasbourg (UNISTRA), Université de Reims Champagne-Ardenne (URCA), Département Sciences Analytiques et Interactions Ioniques et Biomoléculaires (DSA-IPHC), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Institut Français de la Vigne et du Vin (IFV)
Subjects: 0106 biological sciences, 0301 basic medicine, Vine, Sodium arsenite, Physiology, Arsenites, [SDV]Life Sciences [q-bio], Biological pest control, Plant Science, Biology, Photosynthesis, 01 natural sciences, Plant Roots, Polymerase Chain Reaction, 03 medical and health sciences, chemistry.chemical_compound, Sciences du Vivant [q-bio]/Sciences agricoles, [CHIM]Chemical Sciences, Vitis, Mode of action, ComputingMilieux_MISCELLANEOUS, Plant Diseases, Plant Stems, Plant physiology, Pesticide, Sodium Compounds, Fungicide, Plant Leaves, 030104 developmental biology, chemistry, 13. Climate action, Agronomy and Crop Science, 010606 plant biology & botany
Abstract: Sodium arsenite (NaAsO2) was especially used as a dormant spray to control grapevine trunk diseases (GTDs) in European vineyards until 2003 when it was banned. It was an efficient product but it was banned due to high risk for human health and the environment. Now, as one of the consequences with climatic changes, GTDs threaten the sustainability of vineyards since no similar and efficacious sprays are presently available to reduce the impact of GTDs. Research efforts were devoted to identify other active ingredients and biological control agents but they remained limited in term of efficacy. New solutions might follow from a better understanding of the modes of action of sodium arsenite which are currently lacking, specially its impact on grapevine physiology. For this study, grafted plants cv. Tempranillo were sprayed by sodium arsenite at the end of the winter. During the vegetative period, the impact on plant physiology was studied by measurement of the photosynthetic activity, the vine growth and development, and some defense responses. Our results showed that arsenic was translocated throughout the vine with an increasing gradient from the leaves to the root system, that photosynthesis was firstly reduced and then stimulated, and that plant tolerance responses were induced especially antioxidant system. The activation of grapevine defense responses by sodium arsenite could be a complementary action to fight fungal pathogens in addition to the fungicide effect.
Published: 2019

6. Clone-Dependent Expression of Esca Disease Revealed by Leaf Metabolite Analysis

Author: Sophie Trouvelot, Christian Coelho, Marielle Adrian, Gilles Clément, Florence Fontaine, Guillaume Morvan, Claire Grosjean, Jonathan Negrel, Florian Moret, Christelle Lemaître-Guillier, Lucile Jacquens, Grégory Mouille, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Chambre d’Agriculture de Bourgogne Franche-Comté, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Institut Universitaire de la Vigne et du Vin 'Jules Guyot' (IUVV Jules Guyot), Université de Bourgogne (UB), Procédés Alimentaires et Microbiologiques [Dijon] (PAM), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Chambre d'Agriculture de l'Yonne (CA 89), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Adrian, Marielle, Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, and European Union (Feder program), Region Bourgogne Franche Comte, Bureau Interprofessionnel des Vins de Bourgogne
Subjects: 0106 biological sciences, 0301 basic medicine, [SDV]Life Sciences [q-bio], Esca, Clone (cell biology), Context (language use), Disease, Plant Science, Biology, lcsh:Plant culture, 01 natural sciences, 03 medical and health sciences, Metabolomics, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Metabolome, lcsh:SB1-1110, Cultivar, grapevine trunk diseases, ComputingMilieux_MISCELLANEOUS, Original Research, 2. Zero hunger, clone, food and beverages, Metabolite analysis, metabolomics, Vitis vinifera, 3D fluorescence, Horticulture, 030104 developmental biology, Shoot, [SDV.IB]Life Sciences [q-bio]/Bioengineering, 010606 plant biology & botany
Abstract: International audience; Grapevine trutk diseases, especially Esca, are of major concern since they gradually alter vineyards worldwide and cause heavy economic losses. The expression of Esca disease symptoms depends on several factors, including the grapevine cultivar. In this context, a possible clone-dependent expression of the Esca disease was studied. Two clones of 'Chardonnay' grown in the same plot were compared according to their developmental and physiological traits, metabolome, and foliar symptom expression. Analysis of their leaf metabolome highlighted differences related to symptom expression. Interestingly, the content of a few specific metabolites exhibited opposite variations in leaves of symptomatic shoots of clones 76 and 95. Altogether this study showed a clone-dependent expression of Esca disease in 'Chardonnay' and the relevance of GC-MS and 3D fluorescence methods to analyze the impact of the disease on the leaf metabolome.
Published: 2019

7. L’holobionte « vigne » face au dépérissement ou quand le « vivre ensemble » n’est plus à l’équilibre

Author: Sophie TROUVELOT, Héloise Mahé, Pauline Bettenfeld, Evert van Schaik, Marielle Adrian, François Dal, Olivier Fernandez, Florence Fontaine, Claire Grosjean, Philippe Larignon, Gregory Mouille, Fabrice Martin, Sylvain Pabion, Olivier Zekri, Samuel Mondy, Daniel Wipf, Pierre-Emmanuel Courty, ProdInra, Migration, Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Comité National des Interprofessions des Vins, Partenaires INRAE, Université de Reims Champagne-Ardenne (URCA), Sicavac, Chambre Régionale d’Agriculture de Bourgogne Franche-Comté (CRA BFC), Institut Français de la Vigne et du Vin (IFV), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Château de Marsannay, and Pépinières Mercier Frères
Subjects: [SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology
Abstract: Prod 2019-165 SPE EA IPM BIOmE GenoSol INRA UB; National audience
Published: 2019

8. Methods to understand and develop strategies for diagnosis and sustainable control of grapevine trunk diseases

Author: Philippe Larignon, Patricia Trotel-Aziz, R. Pierron, Eliane Abou-Mansour, Sibylle Farine, Florence Fontaine, Philippe Lecomte, A. Jacques, Cecília Rego, Christophe Bertsch, Pedro Reis, Universidade de Lisboa (ULISBOA), Stellenbosch University, Institut Français de la Vigne et du Vin (IFV), Santé et agroécologie du vignoble (UMR SAVE), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), Département de Biologie, Université de Fribourg, Laboratoire Vigne Biotechnologie et Environnement EA 3991, Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA)), Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université de Reims Champagne-Ardenne (URCA), Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0106 biological sciences, 0301 basic medicine, Vine, [SDV]Life Sciences [q-bio], Plant Science, Phaeomoniella chlamydospora, Biology, vineyard, 01 natural sciences, Vineyard, esca disease, Plantlet, 03 medical and health sciences, Cutting, callus, grafted plant, Cultivar, Botryosphaeria dieback, cutting, ComputingMilieux_MISCELLANEOUS, Botrytis cinerea, 2. Zero hunger, pruning wound, fungi, food and beverages, 15. Life on land, biology.organism_classification, cell suspension, Horticulture, 030104 developmental biology, [SDE]Environmental Sciences, Eutypa dieback, plantlet, Agronomy and Crop Science, Powdery mildew, 010606 plant biology & botany
Abstract: International audience; Vitis vinifera is affected by many diseases every year, depending on causal agents, susceptibility of cultivars, and climate region. Some are caused by a single agent, such as gray mold caused by Botrytis cinerea or powdery mildew caused by Erysiphe necator. Others result from the actions of a complex of pathogens such as grapevine trunk diseases (GTDs). GTDs are presently among the most devastating diseases in viticulture worldwide because both the economic losses and the long-term sustainability of vineyards are strongly affected. The complexity of GTDs results from the diversity of associated fungi, the undetermined period of latency within the vine (asymptomatic status), the erratic foliar symptom expression from one year to the next, and, probably correlated with all of these points, the lack of efficient strategies to control them. Distinct methods can be beneficial to improve our knowledge of GTDs. In vitro bioassays with cell suspensions, calli, foliar discs, full leaves, or plantlets, and in vivo natural bioassays with cuttings, grafted plants in the greenhouse, or artificially infected ones in the vineyard, can be applied by using progressive integrative levels of in vitro and in vivo, depending on the information searched. In this review, the methods available to understand GTDs are described in terms of experimental procedures, main obtained results, and deliverable prospects. The advantages and disadvantages of each model are also discussed.
Published: 2019

9. L’holobionte vigne, un bras de levier pour lutter contre les dépérissements ?

Author: Sophie TROUVELOT, Pauline Bettenfeld, Van Scheik, Adrian M, Olivier Fernandez, Florence Fontaine, Grosjean C, Larignon, P., Mouille G, Martin F, Pabion S, Zekri O, Mondy, S., Wipf, D., Courty Pe, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2019

10. ) Clone-dependent expression of grapevine esca disease

Author: Florian Moret, Christelle Guillier, Claire Grosjean, Gilles Clément, Christian Coelho, Lucile Jacquens, Jonathan Negrel, Régis Gougeon, Guillaume Morvan, Gregory Mouille, Sophie TROUVELOT, Florence Fontaine, Marielle Adrian, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Chambre d’Agriculture de Bourgogne Franche-Comté, Institut Universitaire de la Vigne et du Vin 'Jules Guyot' (IUVV Jules Guyot), Université de Bourgogne (UB), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Universidad de la Rioja. Rioja, ESP., Chambre d'Agriculture de l'Yonne (CA 89), Université de Reims Champagne-Ardenne (URCA), Université de Bourgogne Franche-Comté (COMUE) (UBFC). FRA., University of British Columbia (UBC). CAN., and ProdInra, Migration
Subjects: [SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.IDA]Life Sciences [q-bio]/Food engineering, food and beverages, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.IDA] Life Sciences [q-bio]/Food engineering, ComputingMilieux_MISCELLANEOUS
Abstract: International audience; The occurrence of grapevine trunk diseases (GTDs) increases gradually within vineyards, leading to important economic losses. Several factors could affect grapevine susceptibility to GTDs, especially climate, vine age, soil fertilization, and also rootstock, cultivar and clone. For a cultivar, the level of disease expression can vary with region and from year to year. In this context, our objective was to assess if the expression of esca disease, one of the most widespread GTDs, was also clone-dependent. Two clones (76 and 95) of the “Chardonnay” cultivar, grown in the same plot, were compared according to their developmental and physiological traits, metabolome, and esca foliar symptom expression. Agronomical data and symptom expression were recorded during summer 2015, and metabolome analyses were performed in leaf samples collected from visually healthy vines as control (C), and from both symptomatic (D+) and asymptomatic (D-) shoots of esca-affected vines. The bud burst percentage and the fertility ratio were significantly lower for clone 76 than for clone 95, whereas the plant vigor was similar. The percentage of vines expressing the apoplectic and chronic forms of esca disease was low but slightly higher for clone 95. Global GC-MS analysis highlighted a clone-dependent metabolic fingerprint of disease expression. Additional targeted HPLC analyses showed opposite variations in the accumulation of trans-caffeoyltartaric acid, quercetin-O-glucoside, quercetin-O-galactoside, and kaempferol-O-glucoside (higher levels in control leaves of clone 76 than in diseased ones, and the opposite for clone 95). An original approach, 3D fluorescent analysis, was also used to compare samples and also pointed out significant differences in disease expression between clones. Altogether, this study highlights a clone-dependent metabolic response related to esca-disease expression, and the potential of the 3D fluorescence analysis as a new method to detect it. It would be interesting to extend the analysis to other clones and varieties.
Published: 2019

11. Trial with an innovative product based on a biocompatible drug delivery system on GTDs incidence, disease development and vine physiology

Author: Mondello, Vincenzo, Fernandez, Olivier, Trotel-Aziz, Patricia, Christophe, Clément, FLORENCE, FONTAINE, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2019

12. L’holobionte vigne, un levier biologique pour lutter contre les dépérissements ?

Author: Sophie TROUVELOT, Héloise Mahé, Pauline Bettenfeld, Evert van Schaik, Marielle Adrian, François Dal, Olivier Fernandez, Florence Fontaine, Claire Grosjean, Philippe Larignon, Gregory Mouille, Fabrice Martin-Laurent, Sylvain Pabion, Olivier Zekri, Samuel Mondy, Daniel Wipf, Pierre-Emmanuel Courty, Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Comité National des Interprofessions des Vins, Partenaires INRAE, SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Sicavac, Chambre régionale d'agriculture, Institut Français de la Vigne et du Vin (IFV), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Château de Marsannay, Pépinières Mercier Frères, and ProdInra, Migration
Subjects: [SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.IDA] Life Sciences [q-bio]/Food engineering, ComputingMilieux_MISCELLANEOUS
Abstract: National audience
Published: 2019

13. Early season symptoms on grapevine stem and inflorescences associated with Botryophaeriaceae species

Author: P, REIS, A, ALVES, FLORENCE, FONTAINE, I, LOURENCO, Saramago, José, C, REGO, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2019

14. Expression clone-dépendante de l’Esca chez la vigne

Author: Florian Moret, Christelle Guillier, Claire Grosjean, Gilles Clément, Christia Coelho, Lucile JACQUENS, Jonathan Negrel, Régis Gougeon, Guillaume Morvan, Gregory Mouille, Sophie Trouvelot, Florence Fontaine, Marielle Adrian, Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Chambre d’Agriculture de Bourgogne Franche-Comté, Institut National de la Recherche Agronomique (INRA), Université de Bourgogne (UB), Université de Montpellier (UM), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université de Reims Champagne-Ardenne (URCA), and ProdInra, Migration
Subjects: [SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.IDA] Life Sciences [q-bio]/Food engineering
Abstract: National audience; L’incidence des maladies du bois de la vigne (MDB) augmente graduellement au sein des vignobles, causant d’importantes pertes économiques. Le coût mondial du remplacement de vignes malades est estimé à plus d’1,5 milliards de dollars par an [1]. En France, presque 13% du vignoble est affecté [2]. Plusieurs facteurs pourraient influencer la sensibilité aux MDB, notamment le climat, l’âge de la vigne, la fertilisation du sol, le porte-greffe ainsi que le cépage [3]. Actuellement, aucun cépage tolérant aux MDB n’a pu être identifié et le niveau de l’expression des MDB pour un cépage donné peut varier en fonction de la région et d’une année à l’autre [3], et probablement selon notre hypothèse en fonction du clone. Dans ce contexte, notre objectif a été de déterminer si l’expression de l’Esca, une des MDB les plus répandues, était clone-dépendante. Deux clones (76 et 95) du cépage Chardonnay plantés dans la même parcelle ont été comparés selon leurs traits développementaux et physiologiques, leur métabolome, et l’expression foliaire des symptômes d’Esca. Les paramètres agronomiques des vignes furent mesurés durant l’été 2015, et les analyses du métabolome ont été réalisées sur des échantillons de feuilles, dénuées de symptômes foliaires, prélevées sur des vignes asymptomatiques en tant que contrôle (C), et sur des rameaux symptomatiques (D+) et asymptomatiques (D-) de vignes exprimant l’Esca. Les données de GC-MS montrent que l’expression des symptômes d’Esca induit des changements dans le métabolome des feuilles. Certains métabolites présentaient un différentiel d’accumulation entre les feuilles C et D+ au sein de chaque clone. De plus, une différence de profil métabolique entre les feuilles C et D- n’a été observée que pour le clone 95. Enfin, certains métabolites sont inversement accumulés chez les 2 clones pour les échantillons de feuilles D+, avec des quantités plus faibles que chez le contrôle pour le clone 76 et plus élevées pour le clone 95. Il s’agit notamment du kaempferol, kaempferol-3-O-glucoside, gentiobiose et quercétine. La complexité de ces résultats met en avant la difficulté de détecter, chez le Chardonnay, une empreinte métabolique commune aux 2 clones testés associée à l’expression de l’Esca. Enfin, une analyse par fluorescence 3D a également permis de discriminer les échantillons D+ de chaque clone (et de leur contrôle), montrant ainsi le potentiel de cette méthodologie pour étudier l’impact de l’esca sur le métabolome foliaire. Ainsi, cette étude montre que des clones d’un même cépage peuvent répondre, d’un point de vue métabolique, de façon différente à une atteinte d’Esca. Elle sera prochainement publiée dans la revue Frontiers in Plant Science.
Published: 2018

15. Grapevine Trunk Diseases: A Review of Fifteen Years of Trials for Their Control with Chemicals and Biocontrol Agents

Author: Christophe Clément, Patricia Trotel-Aziz, Cátia Pinto, Florence Fontaine, Cindy Coppin, Aurélie Songy, Enrico Battiston, Laura Mugnai, Vincenzo Mondello, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0106 biological sciences, 0301 basic medicine, [SDV]Life Sciences [q-bio], Biological pest control, Plant Science, 01 natural sciences, 03 medical and health sciences, Ascomycota, Antibiosis, Vitis, Natural enemies, Organic Chemicals, Botryosphaeria, ComputingMilieux_MISCELLANEOUS, Plant Diseases, 2. Zero hunger, biology, business.industry, Outbreak, biology.organism_classification, Wood, 3. Good health, Biotechnology, 030104 developmental biology, Inorganic Chemicals, Host-Pathogen Interactions, Eutypa, France, business, Chemical control, Agronomy and Crop Science, 010606 plant biology & botany
Abstract: Grapevine trunk diseases (GTDs) represent one of the most important problems for viticulture worldwide. Beyond the original causes of this outbreak in some countries like France, the lack of efficient control protocols and the prohibition of using active ingredients such as sodium arsenite and benzimidazoles, until recently used to reduce the impact of some GTDs but deleterious for humans and the environment, have probably worsened the impact of the diseases, leading to increasing economic losses. Since 1990, searches have been made to find efficient tools to control GTDs, testing a wide range of active ingredients and biocontrol agents. This review provides readers with an overview of the results reported in the scientific literature over the last 15 years. In particular, the review focuses on the trials carried out applying chemicals or microorganisms to control Esca complex diseases, Botryosphaeria dieback, and Eutypa dieback, the most widespread GTDs.
Published: 2018

16. Grapevine and fungal trunk pathogens interactions and the global impacts of climatic events

Author: Songy, Aurelie, Pinto, Catia, Trotel-Aziz, Patricia, Fernandez, Olivier, Clément, Christophe, FLORENCE, FONTAINE, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2018

17. International congress on grapevine and wine sciences

Author: Songy, Aurelie, Larignon, P, Guise, Jean-Francois, Yobrégat, O., Pinto, Catia, Mondello, Vincenzo, Christophe, Clement, Coppin, Cindy, FLORENCE, FONTAINE, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2018

18. Principaux résultats de l’expérimentation arsénite de sodium au vignoble

Author: FLORENCE, FONTAINE, Larignon, P, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2018

19. Defense Responses in Grapevine (cv. Mourvèdre) after Inoculation with the Botryosphaeria Dieback Pathogens Neofusicoccum parvum and Diplodia seriata and Their Relationship with Flowering

Author: Alessandro Spagnolo, Vincenzo Mondello, Philippe Larignon, Sandra Villaume, Fanja Rabenoelina, Christophe Clément, Florence Fontaine, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: flowering, Gene Expression Profiling, [SDV]Life Sciences [q-bio], food and beverages, Article, lcsh:Chemistry, lcsh:Biology (General), lcsh:QD1-999, Ascomycota, Diplodia seriata, Gene Expression Regulation, Plant, Host-Pathogen Interactions, defense responses, Vitis, Botryosphaeria dieback, Neofusicoccum parvum, lcsh:QH301-705.5, ComputingMilieux_MISCELLANEOUS, Disease Resistance, Plant Diseases
Abstract: As a result of the increasing economic impact of grapevine trunk diseases on viticulture worldwide, efficient and viable control strategies are urgently needed. However, understanding both plant-pathogen interactions and plant physiological changes related to these diseases is fundamental to such an achievement. In this study, we analyzed the effect of inoculation with the Botryosphaeria dieback fungal agents, Neofusicoccum parvum and Diplodia seriata, with and without inflorescence removal at the onset of G stage (separated clusters), I stage (flowering) and M stage (veraison). A measure of lesion size and real-time reverse-transcription polymerase chain reaction-based analysis were carried out. The results clearly show the importance of inflorescences in the development of lesions associated with Botryosphaeria dieback pathogens inoculated on green stems of adult vines, especially at the onset of flowering. At flowering, the biggest necroses were observed with the inflorescences present, as well as an activation of the studied defense responses. Thus, an ineffective response to the pathogen could be consistent with a possible metabolic reprogramming linked to the host phenophase.
Published: 2017

20. Alterations in Grapevine Leaf Metabolism Occur Prior to Esca Apoplexy Appearance

Author: Marielle Adrian, Christophe Clément, Patricia Letousey, Mourad Harir, Alessandro Spagnolo, Lucile Jacquens, Armelle Vallat, Fanja Rabenoelina, Philippe Schmitt-Kopplin, Chloé Roullier-Gall, Florence Fontaine, Eliane Abou-Mansour, Maryline Magnin-Robert, Sophie Trouvelot, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, German Research Center for Environmental Health - Helmholtz Center München (GmbH), Chair of Analytical Food Chemistry, Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM), Université de Neuchâtel (UNINE), Plant Biology Department, and Cornell University [New York]
Subjects: 0106 biological sciences, 0301 basic medicine, Physiology, [SDV]Life Sciences [q-bio], Biology, 01 natural sciences, Mass Spectrometry, Transcriptome, 03 medical and health sciences, chemistry.chemical_compound, Metabolomics, Gene Expression Regulation, Plant, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Vitis, RNA, Messenger, Genetic Association Studies, In Situ Hybridization, Plant Diseases, Plant Proteins, Regulation of gene expression, Fourier Analysis, Gene Expression Profiling, fungi, Fungi, Reproducibility of Results, food and beverages, General Medicine, Metabolism, Glutathione, Cyclotrons, Plant Leaves, Gene expression profiling, 030104 developmental biology, Biochemistry, chemistry, Shoot, [SDE]Environmental Sciences, Metabolome, Phloem, Agronomy and Crop Science, Metabolic Networks and Pathways, 010606 plant biology & botany
Abstract: IPM SPE UB; International audience; Esca disease is one of the major grapevine trunk diseases in Europe and the etiology is complex, since several inhabiting fungi are identified to be associated with this disease. Among the foliar symptom expressions, the apoplectic form may be distinguished and characterized by sudden dieback of shoots, leaf drop, and shriveling of grape clusters in a few days that can ultimately induce the plant death. To further understand this drastic event, we conducted transcriptomic and metabolomic analyses to characterize responses of leaves during the period preceding symptom appearance (20 and 7 days before foliar symptom expression) and at the day of apoplexy ex- pression. Transcriptomic and metabolomic analyses provide signatures for the apoplectic leaves and most changes concerning the metabolism of carbohydrates, amino acids, and phenyl- propanoids. In deciphering glutathione-S-transferase (GST), its preferential location in phloem, correlated with the upregulation of GST genes and a decrease of the glutathione level, offers further support to the putative role of glutathione during apo- plexy expression.
Published: 2017

21. Physiological, molecular and chemical alterations related to esca-leaf symptoms in cv. Tempranillo

Author: L, Martin, FLORENCE, FONTAINE, Ferrer-Gallego, Raúl, R, Roda, F, Castano, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2017

22. Etude de l’impact d’un stress température sous deux régimes hydriques sur la physiologie de la vigne en lien avec l’agressivité des Botryosphaeriaceae

Author: Songy, Aurelie, Larignon, P, FLORENCE, FONTAINE, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2017

23. Grapevine trunk diseases: state of art of scientific research

Author: FLORENCE, FONTAINE, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2017

24. Comparison f the wood-microbiome from grapevine trunk disease-plants, treated or not with sodium arsenite

Author: Bruez, E., Larignon, P, Bertsche, D., P, REY, FLORENCE, FONTAINE, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2017

25. Main results of the COST Action FA1303, Sustainable control of GTDs

Author: FLORENCE, FONTAINE, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2017

26. Deep analysis of the grapevine associated microorganisms: their biocontrol capacity against Botryosphaeria dieback agents grapevine colonisation and biotechnological potential

Author: de Carvalho Pinto, Cátia R. S., Custódio, V, Songy, Aurélie, Gantet, Marie, Clement, Christophe, Gomes, A.C., FLORENCE, FONTAINE, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2017

27. Metabolomic and cytological approaches to better understand grapevine trunk diseaeses disorders

Author: Sophie Trouvelot, Christelle Guillier, Lucile JACQUENS, Antonin Douillet, Samuel Marchat, Claire Grosjean, Chloé Rouiller-Gall, Philippe Schmitt-Kopplin, Philippe Larignon, Florence Fontaine, Marielle Adrian, ProdInra, Migration, Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Research Unit Analytical BioGeoChemistry (BGC), Helmholtz-Zentrum München (HZM), Chair of Analytical Food Chemistry, Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM), Institut Français de la Vigne et du Vin (IFV), Unité de Recherche Vigne et Vins de Champagne Stress et Environnement - EA 4707 (URVVC), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), and Université de Reims Champagne Ardenne (URCA). FRA.
Subjects: [SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract: National audience
Published: 2017

28. Effet d’un produit à base d’extraits de plantes et d’oligoéléments véhiculés par un transporteur pour contrôler les maladies du bois

Author: Mondello, Vincenzo, Clément, Christophe, FLORENCE, FONTAINE, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2017

29. )State of art of scientific research on GTDs. Journée « Grapevine trunk diseases and Flavescence dorée: knowledge and control »

Author: FLORENCE, FONTAINE, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2017

30. Variation amongst Tempranillo clones in susceptibility to Neofusicoccum parvum

Author: C, Berlanas, Songy, Aurelie, Christophe, Clement, FLORENCE, FONTAINE, Gramaje, D., Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2017

31. Arsenite experiments in the program CASDAR V1301 – Arsenic speciation in grapevines and impact on fungal population

Author: Goddard, Mary-Lorène, Boos, A., Larignon, P, FLORENCE, FONTAINE, Bertsche, C., Tarnus, C., Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2017

32. Maladies du bois de la vigne : mise au point d’un modèle simplifié en vue de tester de nouveaux moyens de lutte

Author: FLORENCE, FONTAINE, JF, Chollet, Spagnolo, Alessandro, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2017

33. ). Imaging methods to evaluate rootstock colonization by a GTD related pathogen and its control in grapevine nursery process

Author: Battistoni, C., Compant, S., FLORENCE, FONTAINE, Di,, Mugnai, L, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2017

34. ). Does flowering influence the grapevine defense response towards Botryosphaeriaceous fungi infection ?

Author: Mondello, Vincenzo, Spagnolo, Alessandro, Larignon, P, Villaume, Sandra, Rabenoelina, Fanja, Christophe, Clement, FLORENCE, FONTAINE, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2017

35. An inter-laboratory comparison of DNA barcoding protocols for identifying fungi involved in grapevine trunk disease

Author: Woodhall, J., Perkins, K, Reiss, P., A, Alves, Eichmeier, A., G, Romanazi, C, Kovacs, E, Karaffa, K, Vaczy, Mugnai, L., A, Carlucci, R, Munoz, Gramaje, D., Armengol, J., FLORENCE, FONTAINE, Pinto, Cátia, Synthèse, Structure et Propriétés de Matériaux Fonctionnels (STEP), SYstèmes Moléculaires et nanoMatériaux pour l’Energie et la Santé (SYMMES), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut Nanosciences et Cryogénie (INAC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Università degli Studi di Firenze = University of Florence (UniFI), Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie du CNRS (INC)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Università degli Studi di Firenze = University of Florence [Firenze] (UNIFI), and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2017

36. The effects of grapevine trunk diseases (GTDs) on vine physiology

Author: Julie Vallet, Ana C. Gomes, Alessandro Spagnolo, Florence Fontaine, Christophe Clément, Cátia Pinto, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0106 biological sciences, 0301 basic medicine, [SDV]Life Sciences [q-bio], Plant Science, Horticulture, Carbohydrate metabolism, Biology, Photosynthesis, 01 natural sciences, 03 medical and health sciences, Detoxification, Botany, Botryosphaeria, ComputingMilieux_MISCELLANEOUS, 2. Zero hunger, chemistry.chemical_classification, Reactive oxygen species, Gummosis, food and beverages, Xylem, biology.organism_classification, 030104 developmental biology, chemistry, Eutypa, Agronomy and Crop Science, 010606 plant biology & botany
Abstract: Esca disease as well as Botryosphaeria and Eutypa dieback cause considerable economic problems for vineyards worldwide, and currently, no efficient treatment is available to control these diseases. For these three grapevine trunk diseases (GTDs), the main physiological effects reported concern carbohydrate metabolism and defence responses in the different organs of vine. In the trunk, a depletion of starch reserves in woody tissues is associated with fungal colonization; in the leaves, where pathogens are not present, the carbohydrate metabolism is also affected as revealed by a decline of the photosynthetic rate. A consequence of these disturbances is a lower pool of carbon reserves that might contribute to a decrease of plant development and vigour during the subsequent year. Other metabolic activities such as lipid and amino acid metabolism are down regulated. The perturbation of these primary metabolisms is often associated with the induction of defence responses. The development of biochemical barriers resulting from the accumulation of both tyloses and gummosis is observed during the infection of the wood causing blockage of the xylem vessels and thus limiting the fungal invasion. Their progression in the wood is also inhibited by the formation of polyphenol-rich reaction zones and by the accumulation of pathogenesis-related proteins, and the oxidative burst and the production of reactive oxygen species. Additionally, detoxification processes of the vine are involved; this reaction could be linked to the production of extracellular compounds by GTD agents some of which are phytotoxic. As a consequence, the sensory quality of berries and probably the wine made from these berries decrease. This review presents an overview of the physiological modifications described in vines affected by GTDs.
Published: 2016

37. Grapevine trunk diseases: setting-up of a simple study model for the evaluation of new control strategies

Author: Spagnolo, Alessandro, H, Wu, JF, Chollet, Christophe, Clement, FLORENCE, FONTAINE, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2016

38. State of art of scientific research on GTDs

Author: FLORENCE, FONTAINE, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2016

39. Toxicity of extracellular proteins from Diplodia seriata and Neofusicoccum parvum involved in grapevine Botryosphaeria dieback

Author: F. Mazet-Kieffer, C. Tarnus, Sibylle Farine, M. Goddard, Christophe Bertsch, Elodie Stempien, Hélène Laloue, Julie Chong, Florence Fontaine, M. Bénard-Gellon, Philippe Larignon, Marjorie Schmitt, Flora Pensec, Laboratoire Vigne Biotechnologie et Environnement EA 3991, Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA)), Laboratoire d'innovation moléculaire et applications (LIMA), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), IFV Pôle Rhône-Méditerranée, and Gwangju Institute of Science and Technology (GIST)
Subjects: 0106 biological sciences, [SDV]Life Sciences [q-bio], Diplodia seriata, Virulence, Context (language use), Plant Science, 01 natural sciences, Microbiology, Fungal Proteins, 03 medical and health sciences, Seriata, Ascomycota, Botany, [CHIM]Chemical Sciences, Vitis, Botryosphaeria, Cells, Cultured, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Disease Resistance, Plant Diseases, 2. Zero hunger, 0303 health sciences, biology, fungi, Cell Biology, General Medicine, Phytotoxin, Botryosphaeriaceae, biology.organism_classification, Host-Pathogen Interactions, Eutypa, 010606 plant biology & botany
Abstract: Botryosphaeria dieback, esca and Eutypa dieback are three economic major grapevine trunk diseases that cause severe yield reduction in vineyards worldwide. The frequency of disease symptoms has increased considerably over the past decade, and no efficient treatment is currently available to control these diseases. The different fungi associated with grapevine trunk diseases mainly induce necrotic wood and characteristic foliar symptoms. In this context, fungi virulence factors and host invasion are not well understood. We hypothesise that extracellular proteins produced by Diplodia seriata and Neofusicoccum parvum, two causal agents associated with Botryosphaeria dieback, are virulence factors responsible for the pathogenicity. In our previous work, we demonstrated that the total extracellular compounds produced by N. parvum induced more necrosis on Chardonnay calli and triggered a different defence gene expression pattern than those produced by D. seriata. Furthermore, this aggressiveness was not clearly correlated with the production of mellein, a characteristic phytotoxin of Botryosphaeriaceae, in our in vitro calli model. To characterise other potential virulence factors and to understand the mechanisms of host invasion by the fungus, we evaluated the profile, quantity and the impact of extracellular proteins produced by these fungi on Vitis vinifera calli necrosis and defence gene expression. Our results reveal that, under the same conditions, N. parvum produces more extracellular proteins and in higher concentrations than D. seriata. With Vitis vinifera cv. Chardonnay cells, we showed that equivalent concentrations of proteins secreted by N. parvum were more aggressive than those of D. seriata in producing necrosis and that they clearly induced more grapevine defence genes.
Published: 2015

40. Presentation of grapevine trunk diseases and their impacts on plant physiology

Author: FLORENCE, FONTAINE, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2015

41. Grapevine trunk diseases : setting-up of a simplified model stydy for the évaluation of new control strategies

Author: Spagnolo, Alessandro, H, Wu, JF, Bonnemain, Christophe, Clement, FLORENCE, FONTAINE, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2015

42. The effects on trunk diseases on vine physiologiy in vineyard

Author: FLORENCE, FONTAINE, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2015

43. L’efficacité de l’arsénite de sodium envers les maladies du bois de la vigne

Author: Goddard, Mary-Lorène, Larignon, P, C, Bertsch, Tarnus, C., FLORENCE, FONTAINE, Unité de Recherche Vigne et Vins de Champagne Stress et Environnement - EA 4707 (URVVC), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2015

44. Does past emergence of epicormic shoots control current composition of epicormic types?

Author: Frédéric Mothe, Bruno Chopard, Didier François, Francis Colin, Jean-Baptiste Morisset, Florence Fontaine, French National Forest Service (ONF), and INRA
Subjects: 0106 biological sciences, Sprouts, Forest management, Quercus petraea, Computed tomography, Quercus robur, Secondary buds, Bud clusters, QUERCUS-PETRAEA, SESSILE OAK, ONTOGENY, TREES, BUDS, Biology, 010603 evolutionary biology, 01 natural sciences, [SDV.SA.SF]Life Sciences [q-bio]/Agricultural sciences/Silviculture, forestry, Botany, medicine, Silviculture, Ecology, Thinning, medicine.diagnostic_test, Forestry, 15. Life on land, biology.organism_classification, Horticulture, Epicormic shoot, Shoot, 010606 plant biology & botany
Abstract: International audience; Context While past studies on epicormics in oak (Quercus sp.) have focused on the effect of thinning on epicormic shoots emergence, the consequences of this emergence on the epicormic ontogeny and future wood quality have rarely been asked. Aims This paper aims to quantifying the relationship between past emergence and current composition of epicormics. Methods Three thinning experiments with Quercus petraea (Matt.) Liebl. or Quercus robur L. were investigated. Epicormic shoots were regularly tallied and epicormic composition recorded recently. Some logs were scanned using X-ray computed tomography (CT). Results Past tallies on both species were mainly and positively related to the current frequency of bud clusters and burls. This was due to the production of buds by epicormic shoots for only certain trees, as evidenced by CT, and mainly to correlated numbers of current epicormic shoots, bud clusters and burls, all originating from a past common set of buds and bud clusters. Conclusion The important tree effect on both species suggests that oak silviculture can be optimized by the early selection of crop trees with few epicormics and/or eventually a first and heavy thinning that helps in spotting remaining individuals being prone to the development of multiple epicormics.
Published: 2012

45. Inflorescence of grapevine (Vitis vinifera L.): a high ability to distribute its own assimilates

Author: Nathalie Vaillant-Gaveau, Geneviève Wojnarowiez, Christophe Clément, Pascale Maillard, Patrick Gross, Florence Fontaine, UNITE DE RECHERCHE VIGNES ET VINS DE CHAMPAGNE - STRESS ET ENVIRONNEMENT - EA2069 (URVV - SE), Université de Reims Champagne-Ardenne (URCA), Ecologie et Ecophysiologie Forestières [devient SILVA en 2018] (EEF), and Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL)
Subjects: 0106 biological sciences, Carbon metabolism, Physiology, 13c labelling, Plant Science, Berry, Biology, Photosynthesis, 01 natural sciences, Fruit set, 03 medical and health sciences, Cutting, Botany, grappe, Vitis, inflorescence, Vitis vinifera, 030304 developmental biology, 0303 health sciences, leaf, Reproduction, [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics, Carbon, Plant Leaves, Inflorescence, Organ Specificity, Fruit, carbon partitioning, 010606 plant biology & botany
Abstract: International audience; The distribution of carbon (C) into whole grapevine fruiting cuttings was investigated during flower development to determine the relative contribution of inflorescence and leaf photoassimilates in the total C balance and to investigate their partitioning towards other plant organs. A 13C labelling procedure was used to label C photoassimilates by leaves and inflorescences in grapevine. Investigations were carried out at various stages of flower/berry development, from separated cluster to fruit set, using grapevine fruiting cuttings with four leaves (Vitis vinifera L. cv. Chardonnay). This is the first study reporting that, during its development, (i) the carbon needs of the inflorescence were met by both leaf and inflorescence photosynthesis, and (ii) the inflorescence amazingly participated significantly to the total C balance of grapevine cuttings by redistributing an important part of its own assimilates to other plant organs. With regard to flowering, 29% of C assimilated by the inflorescence remained in the inflorescence, while partitioning towards the stem reached 42% and, as a lower proportion, 15% in leaves, and 14% in roots.
Published: 2011

46. Tracking rameal traces in sessile oak trunks with X-ray computer tomography: biological bases, preliminary results and perspectives

Author: Florence Fontaine, Frédéric Mothe, Jean-Michel Leban, Jean-Baptiste Morisset, Francis Colin, Charline Freyburger, Laboratoire d'Etudes des Ressources Forêt-Bois (LERFoB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Département Ecologie des Forêts, Prairies et milieux Aquatiques (DEPT EFPA), Institut National de la Recherche Agronomique (INRA), Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS)
Subjects: 0106 biological sciences, Java, ImageJ plugin, Physiology, [SDV]Life Sciences [q-bio], Rameal ontogeny, Plant Science, Biology, 010603 evolutionary biology, 01 natural sciences, Paleontology, Knot (unit), Botany, FAGUS-SYLVATICA, KNOT SIZE, computer.programming_language, Ecology, Thinning, PLANT ARCHITECTURE, Rameal sequences, Epicormic traces, Oblique case, NORWAY SPRUCE, Forestry, PINUS-SYLVESTRIS, 15. Life on land, biology.organism_classification, Black spruce, BLACK SPRUCE, L. KARST, Epicormic shoot, 3D log reconstruction, PROVENTITIOUS EPICORMIC BUDS, QUERCUS-PETRAEA, PICEA-ABIES LOGS, Quercus petraea, Tomography, computer, 010606 plant biology & botany
Abstract: Branch knots and other traces of lateral axes, known as rameal traces, have generally been disregarded within architectural studies based on external observations and their analysis limited to tedious manual dissection methods. Based on non-destructive methods including X-ray CT scanning (XRCTS) and on the ontogenic knowledge progressively accumulated on Quercus petraea, this paper presents (1) the tracking methodology of all rameal traces with XRCTS and the "Gourmands" plugin, and software created downstream, notably the 3D visualisation software "Bil3D"; (2) preliminary results obtained on two Quercus petraea 50 cm-long logs; and (3) potential applications in the fields of biology and ecology. Of approximately 45 sequences of linked rameal traces (composing rameal sequences) per metre, half were directly connected to the pith as horizontal traces of primary epicormic buds, while the other half were connected to a branch by an oblique knot. Horizontal epicormic knots essentially emerged within the tree from the most intensive thinning treatment and led to additional knots in the clear wood. Secondary epicormic shoots may emerge from branch and epicormic knots, leading to a vertical spread of epicormic sites for the former and to more bud clusters for the latter. Engulfment of lateral axes can be quantified. Assumptions on the origin of between-tree variability are proposed. Our methodology opens the way to an exhaustive description of rameal traces. It is relevant for ontogenic and wood quality assessment at the trunk level. It may also be very useful for characterising the potentiality of reiteration and for repairing shoot damage and vegetative regeneration at the crown, collar and even root system level.
Published: 2010

47. Determinants of fenhexamid effectiveness against grey mould on grapevine: respective role of spray timing, fungicide resistance and plant defences

Author: Marie-Laure Panon, Nathalie Vaillant-Gaveau, Anne-Noëlle Petit, Fabienne Baillieul, Pierre Leroux, Christophe Clément, Anne-Sophie Walker, Florence Fontaine, UNITE DE RECHERCHE VIGNES ET VINS DE CHAMPAGNE - STRESS ET ENVIRONNEMENT - EA2069 (URVV - SE), Université de Reims Champagne-Ardenne (URCA), BIOlogie et GEstion des Risques en agriculture (BIOGER), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, and Comité Interprofessionnel du Vin de Champagne (CIVC)
Subjects: 0106 biological sciences, RESISTANCE DES PLANTES, [SDV]Life Sciences [q-bio], TREATMENT EFFECTIVENESS, Defence mechanisms, PLANT DEFENSE RESPONSES, Plant disease resistance, 01 natural sciences, Veraison, 03 medical and health sciences, BOTRYTIS CINEREA, GRAPEVINE, Cross-resistance, 030304 developmental biology, Botrytis cinerea, 2. Zero hunger, 0303 health sciences, biology, fungi, food and beverages, biology.organism_classification, Multiple drug resistance, Fungicide, Horticulture, Agronomy, BUNCH ROT, FUNGICIDE RESISTANCE, Chitinase, biology.protein, Agronomy and Crop Science, RESISTANCE GENETIQUE, 010606 plant biology & botany
Abstract: International audience; Botrytis bunch rot of grapes is mainly controlled by applying fungicides at three crop growth stages: the end of flowering (BBCH 68), bunch closure (BBCH 77) and the beginning of veraison (BBCH 81). The hydroxyanilide derivative fenhexamid is among the most effective fungicides registered to control Botrytis cinerea. Its effectiveness was examined in relation to spray timing, fungicide resistance and defense responses of grapevine. Overall, the earlier fenhexamid was applied, the more effective it was at controlling B. cinerea. Frequencies of B. cinerea strains which were resistant to fungicides were evaluated at harvest. The frequencies of resistant phenotypes were similar among treatments and years with the exception of a class of multidrug resistant strain (MDR 2) whose frequency appeared to increase after fenhexamid applications. If current spray programs including fenhexamid appear to control bunch rot at the current MDR frequency, a propagation of MDR 2 strains might lead to a decline in disease control. Finally, defense responses were studied in grapevine flowers/berries following fenhexamid application. None of the defense processes tested was induced in flowers/berries at stages 68 and 77. Only an increase in chitinase activity was observed in treated-berries at stage 81, suggesting that fenhexamid effectiveness was not related to a stimulation of defense responses.
Published: 2010

48. Initial spacing has little influence on transient epicormic shoots in a 20-year-old sessile oak plantation

Author: Florence Fontaine, Francis Colin, Jean-Louis Druelle, Nicolas Robert, and Revues Inra, Import
Subjects: 040101 forestry, 0106 biological sciences, Ecology, media_common.quotation_subject, Forestry, Context (language use), 04 agricultural and veterinary sciences, 15. Life on land, Biology, 01 natural sciences, Competition (biology), Horticulture, Epicormic shoot, [SDV.SA.SF]Life Sciences [q-bio]/Agricultural sciences/Silviculture, forestry, Botany, 0401 agriculture, forestry, and fisheries, [SDV.SA.SF] Life Sciences [q-bio]/Agricultural sciences/Silviculture, forestry, ComputingMilieux_MISCELLANEOUS, 010606 plant biology & botany, media_common
Abstract: • Epicormics are important defects of oak timber which originate from suppressed buds placed early and develop into epicormic shoots depending on the light available, which in turn depends on stand density. In this context, our objective was to assess the epicormic shoots present in a 20-year-old experimental plantation of sessile oak and to test and quantify the effect of the three initial densities applied: 1333, 2667 and 5333 stems/ha. • In the 3 stand densities, epicormic shoots were mostly transient and were rarely clustered. A significant but negligible effect of the density on the epicormic length and on the variables related to epicormic counts appeared: the longest epicormic shoots were present in densities 1333 and 2667 while their means were significantly lower for density 5333. When considering the tree descriptors, the best explanatory models of the different epicormic counts involved their relative variations, especially the relative increment (RIV) in the product V = HEIGHT.DBH2. More precisely, emergence of epicormic shoots occurred particularly when trees fell below a threshold value of RIV = 0.26. Such trees belonged mainly to the two highest densities: 2667 and 5333 stems/ha. • When considering only epicormics, none of the tested densities can be recommended. Trees which bear the most numerous epicormic shoots will be naturally eliminated by stand competition.
Published: 2008

49. Effect of the herbicide flumioxazin on photosynthetic performance of grapevine (Vitis vinifera L.)

Author: Nathalie Vaillant-Gaveau, Florence Fontaine, Aurélie Bigot, Christophe Clément, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0106 biological sciences, Chlorophyll, Environmental Engineering, Photosystem II, Health, Toxicology and Mutagenesis, Phthalimides, Photosynthesis, 01 natural sciences, Carbon assimilation, Botany, Environmental Chemistry, Vitis, Vitis vinifera, Chlorophyll fluorescence, ComputingMilieux_MISCELLANEOUS, biology, Chemistry, Herbicides, Chlorophyll A, RuBisCO, Public Health, Environmental and Occupational Health, 04 agricultural and veterinary sciences, General Medicine, General Chemistry, Pollution, Benzoxazines, Spectrometry, Fluorescence, Carboxylation, [SDE]Environmental Sciences, 040103 agronomy & agriculture, biology.protein, 0401 agriculture, forestry, and fisheries, Weed, 010606 plant biology & botany
Abstract: Among the herbicides used in vineyards, the pre-emergence soil-applied flumioxazin (fmx) is a recently used molecule that inhibits chlorophyll biosynthesis in weed species. The aim of this work is to further characterize the effects of fmx on the non-target grapevine ( Vitis vinifera L. cv. Chardonnay) using cutting as a model. Several photosynthesis parameters were estimated during 25 days after treatment with various fmx concentrations (from 0.5 mM to 50 mM). Measuring chlorophyll fluorescence it appeared that fmx or a by-product penetrated the plant throughout roots and spread throughout vessels. Besides the initial target, protox, fmx affected other functions related to photosynthesis. Fmx induced a simultaneous drop of both P n , g s and T . Fmx caused stomatal closure, which partially explains the decrease of the net photosynthesis. The decline in F v / F m indicates that the photochemistry of PSII and its ability to reduce the primary acceptor Q A are also affected by fmx in grapevine. Fmx leads to a decrease in the coefficients of both photochemical and non-photochemical quenching. Simultaneous stomatal closure and decrease in the quantum yield of CO 2 assimilation indicate a change in energy metabolism following fmx stress. After ten days of fmx treatment, analyses of the response of net carbon assimilation in leaves to different intercellular CO 2 concentrations have shown a decrease of the maximum carboxylation velocity of RuBP. Stomatal closure, PSII photochemistry, change in energy metabolism and RuBP activity were affected by fmx treatment.
Published: 2007

50. Bilan croissance-qualité d'un essai de mycorhization contrôlée sur chêne pédonculé, 16 ans après plantation

Author: Marc Fournier, Florence Fontaine, Jean Garbaye, Francis Colin, Jean-Louis Churin, Interactions Arbres-Microorganismes (IAM), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Laboratoire d'Etudes des Ressources Forêt-Bois (LERFoB), and AgroParisTech-Institut National de la Recherche Agronomique (INRA)
Subjects: 0106 biological sciences, 2. Zero hunger, CHENE PEDONCULE, [SDV.SA.SF]Life Sciences [q-bio]/Agricultural sciences/Silviculture, forestry, Forestry, 15. Life on land, 010603 evolutionary biology, 01 natural sciences, ComputingMilieux_MISCELLANEOUS, 010606 plant biology & botany
Abstract: Les arbres d'une plantation de Chene pedoncule (Quercus robur L.) de 16 ans dans le Nord-Est de la France, realisee avec des plants mycorhizes ou non par le champignon Paxillus involutus, ont ete etudies du point de vue de la croissance, de l'elagage naturel et des gourmands. Les resultats montrent que la stimulation de croissance initiale se traduit encore par un effet significatif sur la hauteur totale, mais que le traitement fongique n'a plus d'effet sur la hauteur ou le diametre de la premiere branche vivante. Les arbres initialement mycorhizes par Paxillus involutus tendent a avoir plus de gourmands, avec une frequence plus elevee de gourmands groupes. Cependant, la plupart de ces gourmands sont petits et de duree de vie tres limitee, et la conclusion generale est que le gain de croissance procure par la mycorhization controlee n'est en rien compromis par d'eventuels effets secondaires pouvant deprecier la qualite des tiges.
Published: 2003

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