1. Epicutaneous Immunotherapy (EPIT) blocks the allergic esophago-gastro -enteropathy induced by sustaines oral exposure to peanuts in sensitized mice
- Author
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Vincent Dioszeghy, Mélanie Ligouis, Emilie Puteaux, Thibaut Larcher, Yan Cherel, Christophe Dupont, Lucie Mondoulet, Pierre-Henri Benhamou, Franck Letourneur, Véronique Dhelft, ProdInra, Migration, Pépinière Santé Paris Cochin, DBV Technologies, Physiopathologie Animale et bioThérapie du muscle et du système nerveux (PAnTher), Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), U 567, Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris Descartes - Paris 5 (UPD5), and Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)
- Subjects
Allergy ,Anatomy and Physiology ,Arachis ,medicine.medical_treatment ,Administration, Oral ,Immunoglobulin E ,Mice ,Intestinal mucosa ,Immune Physiology ,Molecular Cell Biology ,Eosinophilia ,Enteropathy ,Desensitization (medicine) ,Skin ,Gastrointestinal tract ,Mice, Inbred BALB C ,Multidisciplinary ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,food and beverages ,Medicine ,Female ,medicine.symptom ,Cellular Types ,eosinophilia ,Research Article ,epicutaneous immunotherapy ,Science ,Digestive System Diseases ,Immunology ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,Food allergy ,medicine ,Animals ,Peanut Hypersensitivity ,Biology ,[SDV.BC] Life Sciences [q-bio]/Cellular Biology ,food allergy ,business.industry ,Immunity ,medicine.disease ,Disease Models, Animal ,Desensitization, Immunologic ,biology.protein ,Immunization ,Clinical Immunology ,business ,Spleen - Abstract
BackgroundFood allergy may affect the gastrointestinal tract and eosinophilia is often associated with allergic gastrointestinal disorders. Allergy to peanuts is a life-threatening condition and effective and safe treatments still need to be developed. The present study aimed to evaluate the effects of sustained oral exposure to peanuts on the esophageal and jejunal mucosa in sensitized mice. We also evaluated the effects of desensitization with epicutaneous immunotherapy (EPIT) on these processes.MethodsMice were sensitized by gavages with whole peanut protein extract (PPE) given with cholera toxin. Sensitized mice were subsequently exposed to peanuts via a specific regimen and were then analysed for eosinophilia in the esophagus and gut. We also assessed mRNA expression in the esophagus, antibody levels, and peripheral T-cell response. The effects of EPIT were tested when intercalated with sensitization and sustained oral peanut exposure.ResultsSustained oral exposure to peanuts in sensitized mice led to severe esophageal eosinophilia and intestinal villus sub-atrophia, i.e. significantly increased influx of eosinophils into the esophageal mucosa (136 eosinophils/mm(2)) and reduced villus/crypt ratios (1.6±0.15). In the sera, specific IgE levels significantly increased as did secretion of Th2 cytokines by peanut-reactivated splenocytes. EPIT of sensitized mice significantly reduced Th2 immunological response (IgE response and splenocyte secretion of Th2 cytokines) as well as esophageal eosinophilia (50 eosinophils/mm(2), pConclusionsGastro-intestinal lesions induced by sustained oral exposure in sensitized mice are efficaciously treated by allergen specific EPIT.
- Published
- 2012