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3 results on '"Eléonore Durand"'

1. Live-cell imaging of early events following pollen perception in self-incompatible Arabidopsis thaliana

Author

Vincent Bayle, Chie Kodera, Lucie Riglet, Thierry Gaude, Jonathan Schnabel, Isabelle Fobis-Loisy, Frédérique Rozier, Eléonore Durand, fobis-loisy, isabelle, École normale supérieure de Lyon (ENS de Lyon), Reproduction et développement des plantes (RDP), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Évolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198 (Evo-Eco-Paléo (EEP)), Université de Lille-Centre National de la Recherche Scientifique (CNRS), ERC-2013-CoG-615739, ANR-14-CE11-0021,Invader,Réponse des épidermes végétaux aux intrusions cellulaires(2014), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Évolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198 (Evo-Eco-Paléo), and École normale supérieure - Lyon (ENS Lyon)

Subjects
0106 biological sciences, mechanical pressure, Physiology, [SDV]Life Sciences [q-bio], Arabidopsis, macromolecular substances, Pollen Tube, Plant Science, medicine.disease_cause, 01 natural sciences, hydration threshold, Plant reproduction, [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction, 03 medical and health sciences, Live cell imaging, Pollen, otorhinolaryngologic diseases, medicine, Pollination, compatible and incompatible pollinations, Ovule, Actin, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, pollen- stigma interaction, 0303 health sciences, biology, AcademicSubjects/SCI01210, food and beverages, Cell Biology, [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics, Actin cytoskeleton, biology.organism_classification, Research Papers, Pollen hydration, Stigma (anatomy), Cell biology, [SDV] Life Sciences [q-bio], Perception, Pollen tube, pollen–stigma interaction, 010606 plant biology & botany
Abstract

A live imaging system coupled with self-incompatible Arabidopsis lines highlights the role of stigmatic cells in controlling pollen hydration and in reacting to pollen tube intrusion by remodeling the actin cytoskeleton., Early events occurring at the surface of the female organ are critical for plant reproduction, especially in species with a dry stigma. After landing on the stigmatic papilla cells, the pollen hydrates and germinates a tube, which penetrates the cell wall and grows towards the ovules to convey the male gametes to the embryo sac. In self-incompatible species within the Brassicaceae, these processes are blocked when the stigma encounters an incompatible pollen. Based on the generation of self-incompatible Arabidopsis lines and by setting up a live imaging system, we showed that control of pollen hydration has a central role in pollen selectivity. The faster the pollen pumps water from the papilla during an initial period of 10 min, the faster it germinates. Furthermore, we found that the self-incompatibility barriers act to block the proper hydration of incompatible pollen and, when hydration is promoted by high humidity, an additional control prevents pollen tube penetration into the stigmatic wall. In papilla cells, actin bundles focalize at the contact site with the compatible pollen but not with the incompatible pollen, raising the possibility that stigmatic cells react to the mechanical pressure applied by the invading growing tube.

Published
2020
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2. Turnover of ribosome-associated transcripts from de novo ORFs produces gene-like characteristics available for de novo gene emergence in wild yeast populations

Author

Isabelle Gagnon-Arsenault, Christian R. Landry, Isabelle Hatin, Johan Hallin, Alexandre K. Dubé, Lou Nielly-Thibault, Eléonore Durand, Olivier Namy, Département de Biologie [Québec], Institut de Biologie Intégrative et des Systèmes [Québec] (IBIS)-Université Laval [Québec] (ULaval), Département de Biochimie, Microbiologie et Bioinformatique [Québec, Canada], Université Laval [Québec] (ULaval)-Pavillon Charles-Eugène-Marchand [Québec, Canada] (CHM), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Génomique, Structure et Traduction (GST), Département Biologie des Génomes (DBG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), This work was supported by a Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery grant and a Fonds de recherche Nature et Technologies Quebec (FRQNT) Team Grant (2015-PR-181807) to C.R.L. C.R.L. holds the Canada Research Chair in Evolutionary Cell and Systems Biology., Pavillon Charles-Eugène-Marchand [Québec, Canada] (CHM)-Université Laval [Québec] (ULaval), and Namy, Olivier

Subjects
Transcription, Genetic, Biology, [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Ribosome, Open Reading Frames, 03 medical and health sciences, 0302 clinical medicine, Intergenic region, Gene Expression Regulation, Fungal, Yeasts, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BID.EVO] Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE], Genetics, Protein biosynthesis, Saccharomyces paradoxus, ORFS, Gene, Genetics (clinical), 030304 developmental biology, 0303 health sciences, Reporter gene, Research, [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE], [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, biology.organism_classification, Biological Evolution, Open reading frame, Protein Biosynthesis, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Ribosomes, 030217 neurology & neurosurgery
Abstract

Little is known about the rate of emergence of de novo genes, what their initial properties are, and how they spread in populations. We examined wild yeast populations (Saccharomyces paradoxus) to characterize the diversity and turnover of intergenic ORFs over short evolutionary timescales. We find that hundreds of intergenic ORFs show translation signatures similar to canonical genes, and we experimentally confirmed the translation of many of these ORFs in laboratory conditions using a reporter assay. Compared with canonical genes, intergenic ORFs have lower translation efficiency, which could imply a lack of optimization for translation or a mechanism to reduce their production cost. Translated intergenic ORFs also tend to have sequence properties that are generally close to those of random intergenic sequences. However, some of the very recent translated intergenic ORFs, which appeared

Published
2019
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3. Dominance hierarchy arising from the evolution of a complex small RNA regulatory network

Author

Martin Figeac, Pauline M. Goubet, Alexis Sarazin, Sophie Gallina, Isabelle Fobis-Loisy, Eline Guillon, William Marande, Eléonore Durand, Xavier Vekemans, Hélène Bergès, Sylvain Billiard, Thierry Gaude, Céline Poux, Vincent Castric, Raphaël Méheust, Elisa Prat, Marion Soucaze, Laboratoire de Génétique et Evolution des Populations Végétales, Université de Lille, Sciences et Technologies-Centre National de la Recherche Scientifique (CNRS), IFR128, Department of Biology, Plateforme de génomique fonctionnelle et structurelle [Lille], Institut pour la recherche sur le cancer de Lille [Lille] (IRCL)-Université de Lille, Droit et Santé, Centre National de Ressources Génomiques Végétales (CNRGV), Institut National de la Recherche Agronomique (INRA), Université de Lille, Sciences et Technologies, Reproduction et développement des plantes (RDP), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Dept Biol, Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), Université de Lille, Droit et Santé, ANR Jeunes Chercheurs BRASSIDOM [ANR 11 JSV7-008-01], Bonus Qualite Recherche Universite Lille 1, Region Nord Pas de Calais (program 'projets emergents') [MICRO2], CNRS, Université des Sciences et Technologies (Lille 1) (USTL), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon)

Subjects
EXPRESSION, Models, Molecular, Small RNA, [SDV]Life Sciences [q-bio], Gene regulatory network, Arabidopsis, BRASSICA-CAMPESTRIS, Locus (genetics), Genes, Recessive, S-LOCUS, Gene Expression Regulation, Plant, Genotype, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Gene Regulatory Networks, Allele, Selection, Genetic, Pollination, Gene, Alleles, Phylogeny, ComputingMilieux_MISCELLANEOUS, Genes, Dominant, Genetics, Multidisciplinary, [SDV.GEN.GPO]Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE], IDENTIFICATION, biology, PLANT MICRORNAS, LYRATA, POLLEN, biology.organism_classification, Phenotype, Biological Evolution, Genetic Loci, SELF-INCOMPATIBILITY LOCUS, ARABIDOPSIS-THALIANA, RNA, Small Untranslated
Abstract

The prevention of fertilization through self-pollination (or pollination by a close relative) in the Brassicaceae plant family is determined by the genotype of the plant at the self-incompatibility locus ( S locus). The many alleles at this locus exhibit a dominance hierarchy that determines which of the two allelic specificities of a heterozygous genotype is expressed at the phenotypic level. Here, we uncover the evolution of how at least 17 small RNA (sRNA)–producing loci and their multiple target sites collectively control the dominance hierarchy among alleles within the gene controlling the pollen S -locus phenotype in a self-incompatible Arabidopsis species. Selection has created a dynamic repertoire of sRNA-target interactions by jointly acting on sRNA genes and their target sites, which has resulted in a complex system of regulation among alleles.

Published
2014
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