1. Astaxanthin from Haematococcus pluvialis Prevents Oxidative Stress on Human Endothelial Cells without Toxicity
- Author
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Philippe Régnier, Jorge Bastias, Violeta Rodriguez-Ruiz, Noelia Caballero-Casero, Carmen Caballo, Dolores Sicilia, Axelle Fuentes, Murielle Maire, Michel Crepin, Didier Letourneur, Virginie Gueguen, Soledad Rubio, Graciela Pavon-Djavid, Equipe de recherche sur les relations matrice extracellulaire-cellules (ERRMECe), Fédération INSTITUT DES MATÉRIAUX DE CERGY-PONTOISE (I-MAT), Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine-Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine, Laboratoire de Recherche Vasculaire Translationnelle (LVTS (UMR_S_1148 / U1148)), Université Paris Diderot - Paris 7 (UPD7)-Université Paris 13 (UP13)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Joseph Fourier - Grenoble 1 - Institut universitaire de formation des maîtres - Académie de Grenoble (UJF IUFM Grenoble), Université Joseph Fourier - Grenoble 1 (UJF), and Rodriguez-Ruiz, Violeta
- Subjects
TEAC ,Astaxanthin ,Endothelial Cells ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,Xanthophylls ,[SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences ,Carotenoids ,Article ,Antioxidants ,astaxanthin ,[SDV.SP] Life Sciences [q-bio]/Pharmaceutical sciences ,antioxidants ,HUVEC ,lcsh:Biology (General) ,Oxidative stress ,Human Umbilical Vein Endothelial Cells ,Microalgae ,Humans ,oxidative stress ,Haematococcus pluvialis ,lcsh:QH301-705.5 ,[SDV.BC] Life Sciences [q-bio]/Cellular Biology ,Cells, Cultured ,ComputingMilieux_MISCELLANEOUS - Abstract
Astaxanthin, a powerful antioxidant, is a good candidate for the prevention of intracellular oxidative stress. The aim of the study was to compare the antioxidant activity of astaxanthin present in two natural extracts from Haematococcus pluvialis, a microalgae strain, with that of synthetic astaxanthin. Natural extracts were obtained either by solvent or supercritical extraction methods. UV, HPLC-DAD and (HPLC-(atmospheric pressure chemical ionization (APCI)+)/ion trap-MS) characterizations of both natural extracts showed similar compositions of carotenoids, but different percentages in free astaxanthin and its ester derivatives. The Trolox equivalent antioxidant capacity (TEAC) assay showed that natural extracts containing esters displayed stronger antioxidant activities than free astaxanthin. Their antioxidant capacities to inhibit intracellular oxidative stress were then evaluated on HUVEC cells. The intracellular antioxidant activity in natural extracts was approximately 90-times higher than synthetic astaxanthin (5 μM). No modification, neither in the morphology nor in the viability, of vascular human cells was observed by in vitro biocompatibility study up to 10 μM astaxanthin concentrations. Therefore, these results revealed the therapeutic potential of the natural extracts in vascular human cell protection against oxidative stress without toxicity, which could be exploited in prevention and/or treatment of cardiovascular diseases
- Published
- 2015