1. Monolignol ferulate conjugates are naturally incorporated into plant lignins
- Author
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Bronwen G. Smith, John H. Grabber, Troy Runge, Matthew L. Peck, Heather C. A. Free, Laura E. Bartley, Kirankumar S. Mysore, Richard Sibout, John Ralph, Chengcheng Zhang, Steven D. Karlen, Fachuang Lu, Seonghee Lee, Kate E. Helmich, Dharshana Padmakshan, Philip J. Harris, John C. Sedbrook, Rebecca A. Smith, Department of Bio- chemistry, University of Wisconsin-Madison, Department of Energy Great Lakes Bioenergy Research Center, Michigan State University [East Lansing], Michigan State University System-Michigan State University System, Department of Microbiology and Plant Biology, University of Oklahoma (OU), School of Chemical Sciences, Dublin City University [Dublin] (DCU), School of Biological Sciences [Auckland], University of Auckland [Auckland], Department of Horticultural Science, IFAS (Institute of Food and Agricultural Sciences) Gulf Coast Research and Education Center, University of Florida [Gainesville] (UF), Department of Energy Great Lakes Bioenergy Research Center, School of Biological Sciences, Illinois State University, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Agricultural Research Service , US Department of Agriculture, Dairy Forage Research Center, Department of Biological Systems Engineering, Plant Biology Division, The Samuel Roberts Noble Foundation, School of Biological Sciences [Clayton], Monash University [Clayton], University of Wisconsin, University of Florida [Gainesville], Bartley, Laura E., and Ralph, John
- Subjects
0106 biological sciences ,0301 basic medicine ,cell-walls ,BAHD transferase ,[SDV]Life Sciences [q-bio] ,lignin ,perspective ,macromolecular substances ,Plant Science ,01 natural sciences ,complex mixtures ,DFRC ,structural-characterization ,transferase pmt ,dfrc method ,rice ,lignification ,overexpression ,identification ,biosynthesis ,Cell wall ,03 medical and health sciences ,chemistry.chemical_compound ,Transferase ,Lignin ,monocot ,Grasses ,phylogenetic tree ,Research Articles ,transgenic ,Multidisciplinary ,Chemistry ,fungi ,technology, industry, and agriculture ,food and beverages ,SciAdv r-articles ,monolignol ,3. Good health ,030104 developmental biology ,Biochemistry ,Monolignol ,GC-MS ,010606 plant biology & botany ,Conjugate ,Research Article - Abstract
Plants have convergently evolved to use monolignol ferulate conjugates to produce lignins containing chemically labile backbone esters., Angiosperms represent most of the terrestrial plants and are the primary research focus for the conversion of biomass to liquid fuels and coproducts. Lignin limits our access to fibers and represents a large fraction of the chemical energy stored in plant cell walls. Recently, the incorporation of monolignol ferulates into lignin polymers was accomplished via the engineering of an exotic transferase into commercially relevant poplar. We report that various angiosperm species might have convergently evolved to natively produce lignins that incorporate monolignol ferulate conjugates. We show that this activity may be accomplished by a BAHD feruloyl–coenzyme A monolignol transferase, OsFMT1 (AT5), in rice and its orthologs in other monocots.
- Published
- 2016
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