Search

Your search keyword '"Demars, Julie"' showing total 18 results
Start Over Author "Demars, Julie" Publisher hal ccsd
18 results on '"Demars, Julie"'

1. Genetics of body weight and length of Large White piglets and relationship to maternal performance

Author

Canario, L, Billon, Y, Hébrard, W, Demars, Julie, and Demars, Julie

Subjects
[SDV.GEN.GA] Life Sciences [q-bio]/Genetics/Animal genetics
Abstract

Body weight and body metrics of newborn piglets reflect their level of development. We studied a Large White population raised in a single experimental herd. Survival and growth performance of 10,101 piglets originating from 689 litters were analysed. In 440 of these litters, 1,320 piglets contrasting for birth weight were recorded for body length and circumference. At piglet level, the estimated direct and maternal heritability values were were h²d=0.02 and h²m=0.13 for birth weight, h²d=0.65 and h²m=0.21 for the ponderal index and h²d=0.23 and h²m=0.01 for the ratio of circumference to body length. The genetic correlation between the ponderal index and litter size and gestation length tended to be negative.

Published
2022

2. GENOME WIDE ASSOCIATION STUDY OF GROWTH AND FEED EFFICIENCY TRAITS IN RABBITS

Author

Garreau, Hervé, Labrune, Yann, Chapuis, Hervé, Ruesche, Julien, Riquet, Juliette, Demars, Julie, Benitez, Florence, Richard, François, Gilbert, Hélène, Drouilhet, Laurence, Zemb, Olivier, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-École nationale supérieure agronomique de Toulouse (ENSAT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), H2020 Feed-a-Gene, ITAVI-INRAE, and European Project: 633531,H2020,H2020-SFS-2014-2,Feed-a-Gene(2015)

Subjects
[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, [SDV]Life Sciences [q-bio], feed efficiency, rabbit, SNP, GWAS, genetics, feed efficiency SNP GWAS genetics rabbit
Abstract

International audience; We performed a genome wide association study for growth and feed efficiency using the GEMMA software on 679 rabbits genotyped with the Affimetrix Axiom Rabbit 200K Genotyping Array. No significant SNP was found for growth traits or feed intake. Two and 89 chromosome-wide significant SNPs were detected for feed conversion ratio and residual feed intake, respectively. The 89 significant SNPs for residual feed intake were all located on chromosome 18, where the putative functional candidate gene GOT1 could be identified.

Published
2021

3. Heterologous hybridization using the human exome - a molecular tool to target and identify major genes

Author

Iannuccelli, Nathalie, Cabau, Cédric, Sarry, Julien, Bouchez, Olivier, Billon, Yvon, Riquet, Juliette, Allain, Daniel, Demars, Julie, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, human+exome+capture+sequencing+variants
Abstract

International audience; Identifying causal mutations responsible of phenotypes in a time and cost-effective manner remains a challenge still for all species. Although the portfolio of next-generation sequencing methodologies offers a broad range of opportunities nowadays, having the right and suitable molecular genetic tools in non-model organisms is often an issue. For the European rabbit (Oryctolagus cuniculus), considered as an animal model for experimental research, the OryCun2.0 reference genome was released in 2009 and the Affymetrix AxiomOrcun SNP Array was made available in 2016. This novel beadchip gives the possibility to develop conveniently genome-wide association studies (GWAS) in rabbits without however freeing itself from the subsequent steps of causal mutation characterization. Whole exome sequencing (WES) appears as a direct and suitable strategy to target and sequence variants all at once. Unfortunately, no exome enrichment tool exist for rabbits. Here, we proposed to capture the rabbit exome using the marketed human panel since human and rabbit genomes are closely related on the phylogenetic tree of species. We performed heterologous hybridization for 48 rabbits from 7 different populations and generated 2 billion sequencing reads for the whole dataset. The quality of the heterologous hybridization allowed the capture of the rabbit exome and enabled a dataset of 40,000 confident variants that specifically target both protein-coding and extended exons. This method also presents a unique opportunity to investigate both (i) the genetic diversity of different rabbits’ breeds and (ii) the mendelian determinism of specific phenotypes in rabbits without resorting to costly customized tools.

Published
2021

4. The importance of annotations (reference genome and parent gene) for the study of circRNAs

Author

robic, annie, Faraut, Thomas, Cerutti, Chloé, Demars, Julie, Kühn, Christa, robic, annie, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Leibniz Institute for Farm Animal Biology (FBN), and University of Rostock

Subjects
[SDV] Life Sciences [q-bio], FAANG, annotation, [SDV]Life Sciences [q-bio], circular RNA, livestock species, ComputingMilieux_MISCELLANEOUS, noncoding RNA
Abstract

International audience

Published
2021

5. New perspective on intergenerational transmission of characters

Author

David, Ingrid, Aliakbari, Amir, Canario, Laurianne, Combes, Sylvie, Demars, Julie, Déru, Vanille, Garreau, Herve, Gilbert, Hélène, Ricard, Anne, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and DAVID, Ingrid

Subjects
[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, [SDV.GEN.GA] Life Sciences [q-bio]/Genetics/Animal genetics, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2020

7. A Long Read project to find optimal technologic combinations for genome assembly and variability, epigenetic marks detection and metagenomic analysis

Author

Iampietro, Carole, Eché, Camille, Castinel, Adrien, Serre, Rémy-Félix, Klopp, Christophe, Denis, Erwan, Bouchez, Olivier, Kuchly, Claire, Vandecasteele, Céline, BROHA, Amandine, THERVILLE, Romain, Di Franco, Arnaud, Djebali Quelen, Sarah, DREAU, Andreea, Hoede, Claire, KOROVINA, Oleksandra, BIRBES, CLEMENT, Laborie, Didier, Mainguy, Jean, Noirot, Céline, Salin, Gerald, Terzian, Paul, Trotard, Marie-Stéphane, Boichard, Didier, Boussaha, Mekki, Grohs, Cécile, Charcosset, Alain, Belcram, Harry, Joets, Johann, Combes, Sylvie, Pascal, Géraldine, Pitel, Frederique, Leroux, Sophie, Riquet, Juliette, Demars, Julie, Tosser-Klopp, Gwenola, Vitte, Clémentine, Iannuccelli, Nathalie, Lluch, Jérôme, Lopez-Roques, Celine, Faraut, Thomas, Zytnicki, Matthias, Gaspin, Christine, Milan, Denis, Donnadieu, Cécile, Génome et Transcriptome - Plateforme Génomique (GeT-PlaGe), Institut National de la Recherche Agronomique (INRA)-Plateforme Génome & Transcriptome (GET), Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de Mathématiques et Informatique Appliquées de Toulouse (MIAT INRA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Génétique Animale et Biologie Intégrative (GABI), Université Paris-Saclay-AgroParisTech-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale) (GQE-Le Moulon), AgroParisTech-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), FEDER-Région Occitanie, Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), and AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
[SDV.GEN]Life Sciences [q-bio]/Genetics, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, [SDV]Life Sciences [q-bio], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [INFO]Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2020

8. Intergenerational Transmission of Characters Through Genetics, Epigenetics, Microbiota, and Learning in Livestock

Author

David, Ingrid, Canario, Laurianne, Combes, Sylvie, Demars, Julie, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], lcsh:QH426-470, behavior, Review, culture, non-genetic inheritance, livestock, lcsh:Genetics, genetic, epigenetic, microbiota, Genetics, Autre (Sciences du Vivant)
Abstract

International audience; Evolutionary biologists studying wild species have demonstrated that genetic and non-genetic sources of information are inherited across generations and are therefore responsible for phenotypic resemblance between relatives. Although it has been postulated that non-genetic sources of inheritance are important in natural selection, they are not taken into account for livestock selection that is based on genetic inheritance only. According to the natural selection theory, the contribution of non-genetic inheritance may be significant for the transmission of characters. If this theory is confirmed in livestock, not considering non-genetic means of transmission in selection schemes might prevent achieving maximum progress in the livestock populations being selected. The present discussion paper reviews the different mechanisms of genetic and non-genetic inheritance reported in the literature as occurring in livestock species. Non-genetic sources of inheritance comprise information transmitted via physical means, such as epigenetic and microbiota inheritance, and those transmitted via learning mechanisms: behavioral, cultural and ecological inheritance. In the first part of this paper we review the evidence that suggests that both genetic and non-genetic information contribute to inheritance in livestock (i.e. transmitted from one generation to the next and causing phenotypic differences between individuals) and discuss how the environment may influence non-genetic inherited factors. Then, in a second step, we consider methods for favoring the transmission of non-genetic inherited factors by estimating and selecting animals on their extended transmissible value and/or introducing favorable non-genetic factors via the animals' environment.

Published
2019

9. Les gènes de coloration et leurs interactions, le modèle lapin Himalayan

Author

Demars, Julie, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, and ProdInra, Migration

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], interaction, ComputingMilieux_MISCELLANEOUS, génétique
Abstract

National audience

Published
2018

10. EIF2S2 Retroposition into IRF2BP2 Underlies Fleece Variation in Sheep through the Formation of a Long Endogenous Double-Stranded RNA

Author

Demars, Julie, Cano, Margarita, Drouilhet, Laurence, Fabre, Stéphane, Servin, Bertrand, Mulsant, Philippe, Tosser-Klopp, Gwenola, Allain, Daniel, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, and Instituto Nacional de Tecnología Agropecuaria (INTA)

Subjects
Animal biology, sheep, phenotypic, selection, fiber, production, [SDV.BA]Life Sciences [q-bio]/Animal biology, Biologie animale
Abstract

International audience; Fleece variation, in composition and structure, observed in mammals is a consequence of a strong selective pressure for fiber production after domestication. Wild species with double coated fleece produced a variety of breeds with single coated fleece. In sheep, these two categories discriminate primitive sheep from woolly modern sheep (Ovis aries). Nowadays, fleece variation is segregating in the Romane breed with lambs owning a coat either long and hairy (characteristic of ancestral sheep) or short and woolly (typical to domestic sheep). Here we report that the “woolly” allele results from the insertion of an antisense EIF2S2 (Eukaryotic Translation Initiation Factor 2, Subunit 2 Beta) retrogene (called asEIF2S2) into the 3’ UTR of the IRF2BP2 (Interferon Regulatory Factor 2 Binding Protein 2) gene leading to an abnormal IRF2BP2 transcript. We provide evidence that this chimeric IRF2BP2/asEIF2S2 messenger, by targeting the genuine sense EIF2S2 RNA and creating a long endogenous double-stranded RNA, alters the expression of EIF2S2 mRNA. This is the first example, to our knowledge of a phenotype arising from the modulation of messengers accumulation via a RNA-RNA hybrid itself generated through a retroposition gene mechanism. Altogether our results bring new insights on (i) the mammalian hair morphogenesis with the identification of EIF2S2 and IRF2BP2 as novel key regulators and (ii) the sheep population history thanks to the characterization of the molecular origin of an evolutionary phenotypic variation. Furthermore, these findings will contribute to a sustainable sheep production system since lambs carrying a hairy fleece are more adapted and robust to environmental harsh conditions.

Published
2017

11. Impact du rationnement alimentaire sur le phénotype Himalayan

Author

Demars, Julie, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, and Institut National de Recherche Agronomique (INRA). UAR Département Physiologie Animale et Systèmes d'Elevage (0558).

Subjects
[SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]
Abstract

National audience; Perspectives de travaux à venir Suite à des observations faites sur les lapins de la lignée 1001, des différences de coloration et de surfaces de zones colorées ont été constatées. Ces variations sont principalement observées au niveau des oreilles, du museau et des pattes. Ce phénotype particulier appelé « Himalayan » est également retrouvé chez la souris, le chat et le cobaye. Chez le lapin, un variant dans le gène de la tyrosinase (Tyr) a été identifié mais aucune étude récente n’a permis de valider son implication dans le déterminisme génétique du phénotype et aucune étude n’a permis de montrer que la gamme de variation de la coloration était déterminée par cette mutation. Des données disponibles chez la souris pour le gène Asip indiquent que pour un même génotype des variations phénotypiques de la coloration pouvaient être déterminées par des différences de méthylation en amont de la séquence du gène et qu’un stress alimentaire pouvait induire, via un pattern de méthylation variable, des différences de couleur des animaux. On émet donc l’hypothèse que le phénotype « Himalayan » pourrait être déterminé, en plus de la mutation décrite, par des modifications de type épigénétique et que le rationnement pourrait influencer l’expression du gène responsable de la coloration des extrémités du lapin.

Published
2016

12. Fine mapping of birthcoat type in the Romanov breed sheep

Author

Cano, Margarita, Allain, Daniel, Foulquié, Didier, Moreno, Carole, Mulsant, Philippe, François, Dominique, Demars, Julie, Tosser-Klopp, Gwenola, Instituto Nacional de Tecnología Agropecuaria (INTA), Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), Domaine expérimental de La Fage (LA FAGE), Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées

Subjects
marker, genomic, romane breed sheep, qtl, [SDV]Life Sciences [q-bio], birthcoat, snp, genetic, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2013

13. Mapping a putative autosomal gene controlling ovulation rate and infertility in Cambridge sheep

Author

Keane, O.M., HANRAHAN, J.P., Tosser-Klopp, Gwenola, Sarry, Julien, Fabre, Stéphane, Demars, Julie, Bodin, Loys, Teagasc Agriculture and Food Development Authority (Teagasc), Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
cambridge sheep, [SDV]Life Sciences [q-bio], selection, [INFO]Computer Science [cs], genetic, identifi, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2013

14. Two new mutations affecting ovulation rate in sheep

Author

Demars, Julie, Fabre, Stéphane, Rossetti, Raffaella, Persani, Luca, Tosser-Klopp, Gwenola, Mulsant, Philippe, Nowak, Zuzanna, Drobick, W., Martyniuk, E., Bodin, Loys, Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Laboratorio di Ricerche Endocrino-Metaboliche, Istituto Auxologico Italiano, Università degli Studi di Milano [Milano] (UNIMI), Warsaw University of Life Sciences (SGGW), Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), ANR Monopoly, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
reproduction, sheep, [SDV]Life Sciences [q-bio], snp, [INFO]Computer Science [cs], genetic, mutation, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2012

15. Novel mutations controlling ovulation rate in sheep

Author

Bodin, Loys, Demars, Julie, Drobik, W., Fabre, Stéphane, HANRAHAN, J.P., Keane, O., Martyniuk, E., Mulsant, Philippe, Nowak, Z., Persani, L., Rossetti, R., Sarry, Julien, Tosser-Klopp, Gwenola, Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Warsaw University of Life Sciences (SGGW), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Teagasc Agriculture and Food Development Authority (Teagasc), Warsaw Agricultural University, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Università degli Studi di Milano [Milano] (UNIMI), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
sheep, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], ovulation, mutation, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2012

16. Towards candidate genes affecting body fatness at the SSC7 QTL by expression analyses

Author

Gondret, Florence, Riquet, Juliette, Tacher, Sandrine, Demars, Julie, Sanchez, Marie Pierre, Billon, Yvon, Robic, Annie, Bidanel, Jean Pierre, Milan, Denis, Systèmes d'Elevage, Nutrition Animale et Humaine (SENAH), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure Agronomique de Rennes, Laboratoire de Génétique Cellulaire (LGC), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Génétique Expérimentale en Productions Animales (GEPA), Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, and AgroParisTech-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences, qtl, ppard, cdkn1a, adipogenesis, swine chromosome 7
Abstract

A quantitative trait locus (QTL) affecting fatness in a way opposite to expectations based on breed means was mapped to swine chromosome 7 (SSC7) using crosses between Large White (LW) and Meishan (MS) founders. Defining the molecular fatness trait more explicitly would allow deducing positional candidate genes, for which expression differences must be analysed in experimental populations. First, mRNA levels of genes representing sequential steps in adipogenesis or involved in lipid metabolism were studied in backfat of pigs having homozygous LWQTL7/LWQTL7 or heterozygous LWQTL7/MSQTL7 alleles and considered at two ages. mRNA level of DLK1 expressed in preadipocytes was greater in MSQTL7/LWQTL7 pigs than in homozygous pigs at 28 days. Transcript abundances of CEBPA involved in differentiation, the prolipogenic FASN gene and the adipocyte-specific marker FABP4 were lower in MSQTL7/LWQTL7 pigs compared with LWQTL7/LWQTL7 pigs at 150 days. Because these results suggest a lag time in terminal differentiation associated with the MS allele, seven genes in the QTL interval were deduced as promising candidates for the QTL effect by bioinformatics analysis. Among them, PPARD and CDKN1A had lower expression levels in MSQTL7/LWQTL7 pigs at both ages. Genotype-related differences were observed in mRNA levels of PPARD target genes involved in cell differentiation (FZD7) or fatty acid oxidation (ACADL and ACOX1) at 150 days. These results re-evaluate the potential of PPARD to explain part of variation in pig adiposity.

Published
2012

17. A genome-wide epistatic network underlies the molecular architecture of continuous color variation of body extremities

Author

Julie Demars, Yann Labrune, Nathalie Iannuccelli, Alice Deshayes, Sophie Leroux, Hélène Gilbert, Patrick Aymard, Florence Benitez, Juliette Riquet, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-École nationale supérieure agronomique de Toulouse (ENSAT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Stabilité génétique, cellules souches et radiations (SGCSR (U_1274 / UMR_E_008)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay-Université Paris Cité (UPCité), Département Génétique Animale, INRAE, Laboratoire GenPhySE - Génétique Physiologie et Systèmes d'Elevage, Centro Nacional de Genotipado (CeGen), GenoToul bioinformatics, European Project: 633531,H2020,H2020-SFS-2014-2,Feed-a-Gene(2015), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Demars, Julie, and Adapting the feed, the animal and the feeding techniques to improve the efficiency and sustainability of monogastric livestock production systems - Feed-a-Gene - - H20202015-03-01 - 2020-02-29 - 633531 - VALID

Subjects
epistasis, Melanogenesis, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, LD, epistasis Melanogenesis Recessivity BAF, CNV, LRR, SNP, local false sign rate, Oryctolagus cuniculus, Coat coloration Copy number variation, credible set, single nucleotide polymorphism, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Genetics, LFSR, GWAS, B allele frequency, genome wide association study, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, Copy number variation, Recessivity, [SDV.BA.MVSA] Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, PIP, posterior inclusion probability, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, Coat coloration, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], CS, Ocu, linkage disequilibrium, log R ratio
Abstract

International audience; Deciphering the molecular architecture of coat coloration for a better understanding of the biological mechanisms underlying pigmentation still remains a challenge. We took advantage of a rabbit French experimental population in which both a pattern and a gradient of coloration from white to brown segregated within the himalayan phenotype. The whole experimental design was genotyped using the high density Affymetrix® AxiomOrcun™ SNP Array and phenotyped into 6 different groups ordered from the lighter to the darker. Genome-wide association analyses pinpointed an oligogenic determinism, under recessive and additive inheritance, involving genes already known in melanogenesis (ASIP, KIT, MC1R, TYR), and likely processed pseudogenes linked to ribosomal function, RPS20 and RPS14. We also identified (i) gene-gene interactions through ASIP:MC1R affecting light cream/beige phenotypes while KIT:RPS responsible of dark chocolate/brown colors and (ii) a genome-wide epistatic network involving several others coloration genes such as POT1 or HPS5. Finally, we determined the recessive inheritance of the English spotting phenotype likely involving a copy number variation affecting at least the end of the coding sequence of the KIT gene. Our analyses of coloration as a continuous trait allowed us to go beyond much of the established knowledge through the detection of additional genes and gene-gene interactions that may contribute to the molecular architecture of the coloration phenotype.

Published
2022

18. Deciphering the molecular architecture of the coat colour variability in a European rabbit population

Author

Julie Demars, Yann Labrune, Nathalie Iannuccelli, Patrick Aymard, Florence Benitez, Sophie Leroux, Hélène Gilbert, Juliette Riquet, and Demars, Julie

Subjects
coloration+interaction+epistasis+GWAS+rabbit+recessive, [SDV.GEN.GA] Life Sciences [q-bio]/Genetics/Animal genetics
Abstract

Understanding the molecular mechanism of coloration has been the goal of many genetic and evolutionary studies in a broad number of species. Nevertheless, most of our current knowledge is restricted to colour traits exhibiting relatively simple discrete variation and inheritance patterns. More than a hundred of genes are involved in coloration traits in rodents and many mutations have been identified. In the European rabbit (Oryctolagus cuniculus), different coat colours have been selected through domestication and are nowadays fixed in specific breeds. Although numerous mutations affecting coat colour have been discovered in various rabbit breeds, additional variants have still to be identified. Despite the evolution of technologies through the genomics era, understanding the molecular architecture of such complex phenotypes still remains a challenge. Here, we propose a genome-wide investigation of coat colour using rabbit high-density SNP array. We performed genome-wide association studies (GWAS) considering the variation of coat colour as quantitative and binary phenotypes. We identified several significant SNPs marking loci already known to affect coat colour as well as in a few other chromosome regions not yet described to affect this phenotype in rabbits (e.g. a genomic region on chromosome 14). Moreover, we determined the best model of inheritance for each region associated to coat colour. Our results bring new insights into the molecular architecture of the coloration phenotype pinpointing its oligogenic determinism.

Published
2021

Catalog

Books, media, physical & digital resources
See catalog results