1. Brucella papii sp. nov. isolated from baboons (Papio spp.)
- Author
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Sascha Al Dahouk, Simon D. Brew, Mark S. Koylass, Michel S. Zygmunt, Natalia Schlabritz-Loutsevitch, Gene B. Hubbard, Adrian M. Whatmore, Edward J. Dick, Lorraine L. Perrett, Holger C. Scholz, Christine Quance, Nicholas J. Davison, Axel Cloeckaert, Gilles Vergnaud, WHO/FAO/OIE, Animal Health and Veterinary Laboratories Agency, Université Francois Rabelais [Tours], Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Federal Institute for Risk Assesment, Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), CNRS, F-91405 Orsay, France, Centre National de la Recherche Scientifique (CNRS), Mission pour la Recherche et l'Innovation Scientifique (DGA/MRIS), Ministère de la défense, DGA : délégation générale de l'armement, Mycobacteria and Brucella Section (USDA-APHIS), National Veterinary Services Laboratories, Bundeswehr Institute of Microbiology, Southwest National Primate Research Center, Texas Biomedical Research Institute, Department of Pathology, University of Texas Health Science Center, The University of Texas Health Science Center at Houston (UTHealth)- The University of Texas Health Science Center at Houston (UTHealth), Department of obstetrics and gynecology, Infectiologie Santé Publique (ISP-311), Université de Tours-Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)
- Subjects
DNA, Bacterial ,New Taxa ,040301 veterinary sciences ,Sequence analysis ,Molecular Sequence Data ,Locus (genetics) ,Brucella ,Multiple Loci VNTR Analysis ,Microbiology ,Brucellosis ,0403 veterinary science ,Bacteriophage ,03 medical and health sciences ,Tandem repeat ,RNA, Ribosomal, 16S ,Animals ,Ecology, Evolution, Behavior and Systematics ,Phylogeny ,Genetics ,0303 health sciences ,[SDV.GEN]Life Sciences [q-bio]/Genetics ,biology ,030306 microbiology ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,Nucleic Acid Hybridization ,Baboon ,04 agricultural and veterinary sciences ,General Medicine ,Sequence Analysis, DNA ,Brucella papionis ,16S ribosomal RNA ,biology.organism_classification ,bacterial infections and mycoses ,Bacterial Typing Techniques ,Genes, Bacterial ,Female ,Multilocus Sequence Typing ,Papio - Abstract
Two Gram-negative, non-motile, non-spore-forming coccoid bacteria (strains F8/08-60T and F8/08-61) isolated from clinical specimens obtained from baboons (Papio spp.) that had delivered stillborn offspring were subjected to a polyphasic taxonomic study. On the basis of 16S rRNA gene sequence similarities, both strains, which possessed identical sequences, were assigned to the genus Brucella . This placement was confirmed by extended multilocus sequence analysis (MLSA), where both strains possessed identical sequences, and whole-genome sequencing of a representative isolate. All of the above analyses suggested that the two strains represent a novel lineage within the genus Brucella . The strains also possessed a unique profile when subjected to the phenotyping approach classically used to separate species of the genus Brucella, reacting only with Brucella A monospecific antiserum, being sensitive to the dyes thionin and fuchsin, being lysed by bacteriophage Wb, Bk2 and Fi phage at routine test dilution (RTD) but only partially sensitive to bacteriophage Tb, and with no requirement for CO2 and no production of H2S but strong urease activity. Biochemical profiling revealed a pattern of enzyme activity and metabolic capabilities distinct from existing species of the genus Brucella . Molecular analysis of the omp2 locus genes showed that both strains had a novel combination of two highly similar omp2b gene copies. The two strains shared a unique fingerprint profile of the multiple-copy Brucella-specific element IS711. Like MLSA, a multilocus variable number of tandem repeat analysis (MLVA) showed that the isolates clustered together very closely, but represent a distinct group within the genus Brucella . Isolates F8/08-60T and F8/08-61 could be distinguished clearly from all known species of the genus Brucella and their biovars by both phenotypic and molecular properties. Therefore, by applying the species concept for the genus Brucella suggested by the ICSP Subcommittee on the Taxonomy of Brucella, they represent a novel species within the genus Brucella , for which the name Brucella papionis sp. nov. is proposed, with the type strain F8/08-60T ( = NCTC 13660T = CIRMBP 0958T).
- Published
- 2014
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