1. Prebiotic Supplementation During Pregnancy Modifies the Gut Microbiota and Increases Metabolites in Amniotic Fluid, Driving a Tolerogenic Environment In Utero
- Author
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Brosseau, Carole, Selle, Amandine, Duval, Angeline, Misme-Aucouturier, Barbara, Chesneau, Melanie, Brouard, Sophie, Cherbuy, Claire, Cariou, Véronique, Bouchaud, Gregory, Mincham, Kyle, Strickland, Deborah, Barbarot, Sebastien, Bodinier, Marie, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre de Recherche en Transplantation et Immunologie (U1064 Inserm - CRTI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), The University of Western Australia (UWA), Physiopathologie des Adaptations Nutritionnelles (PhAN), Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), French government (Agence National pour la Recherche), CHU de Nantes., École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), and Bodinier, Marie
- Subjects
immune tolerance ,Placenta ,Immunology ,B-Lymphocyte Subsets ,Oligosaccharides ,Acetates ,Ribotyping ,Feces ,Mice ,Fetus ,[SDV.MHEP.PED] Life Sciences [q-bio]/Human health and pathology/Pediatrics ,T-Lymphocyte Subsets ,Animals ,Humans ,Maternal-Fetal Exchange ,Original Research ,Mice, Inbred BALB C ,[SDV.MHEP.PED]Life Sciences [q-bio]/Human health and pathology/Pediatrics ,gut microbiota ,Uterus ,Inulin ,Pregnancy Outcome ,Dendritic Cells ,Amniotic Fluid ,Gastrointestinal Microbiome ,Butyrates ,[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition ,feto-maternal tissues ,Prebiotics ,Prenatal Exposure Delayed Effects ,Dietary Supplements ,prebiotic ,Pregnancy, Animal ,Female ,pregnancy ,immune imprinting ,Propionates ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition - Abstract
International audience; The gut microbiota is influenced by environmental factors such as food. Maternal diet during pregnancy modifies the gut microbiota composition and function, leading to the production of specific compounds that are transferred to the fetus and enhance the ontogeny and maturation of the immune system. Prebiotics are fermented by gut bacteria, leading to the release of short-chain fatty acids that can specifically interact with the immune system, inducing a switch toward tolerogenic populations and therefore conferring health benefits. In this study, pregnant BALB/cJRj mice were fed either a control diet or a diet enriched in prebiotics (Galacto-oligosaccharides/Inulin). We hypothesized that galacto-oligosaccharides/inulin supplementation during gestation could modify the maternal microbiota, favoring healthy immune imprinting in the fetus. Galacto-oligosaccharides/inulin supplementation during gestation increases the abundance of Bacteroidetes and decreases that of Firmicutes in the gut microbiota, leading to increased production of fecal acetate, which was found for the first time in amniotic fluid. Prebiotic supplementation increased the abundance of regulatory B and T cells in gestational tissues and in the fetus. Interestingly, these regulatory cells remained later in life. In conclusion, prebiotic supplementation during pregnancy leads to the transmission of specific microbial and immune factors from mother to child, allowing the establishment of tolerogenic immune imprinting in the fetus that may be beneficial for infant health outcomes.
- Published
- 2021
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