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1. Boron doped graphene synthesis using pulsed laser deposition and its electrochemical characterization

Author

Carole Chaix, Florence Garrelie, Christophe Donnet, Carole Farre, Florent Bourquard, Jules Galipaud, Anne-Sophie Loir, Y. Bleu, Vincent Barnier, Laboratoire Hubert Curien [Saint Etienne] (LHC), Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS), Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), École Centrale de Lyon (ECL), Université de Lyon, École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), and Institut Mines-Télécom [Paris] (IMT)

Subjects
Materials science, Heteroatom, chemistry.chemical_element, 02 engineering and technology, 010402 general chemistry, Electrochemistry, 01 natural sciences, Pulsed laser deposition, law.invention, [SPI.MAT]Engineering Sciences [physics]/Materials, symbols.namesake, X-ray photoelectron spectroscopy, law, Materials Chemistry, [CHIM]Chemical Sciences, Electrical and Electronic Engineering, Boron, ComputingMilieux_MISCELLANEOUS, Graphene, Mechanical Engineering, General Chemistry, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Electronic, Optical and Magnetic Materials, Chemical engineering, chemistry, Electrode, symbols, 0210 nano-technology, Raman spectroscopy
Abstract

During the design of graphene (G), their electronic, chemical and electrochemical properties among others can be tuned by incorporating heteroatoms in the honeycomb structure. In this study, we investigated boron doped graphene (BG) synthesis and the effect of boron content on the electrochemical property of graphene. To this end, BG was synthesized using pulsed laser deposition (PLD) with carbon and boron as solid sources, followed by rapid thermal annealing in the presence of nickel catalyst. Raman spectroscopy revealed that the BG films consisted of few-layer graphene and boron doping increased the defects level in the films. X-ray photoelectron spectroscopy confirmed the successful boron doping into the graphene with the control of boron content. Electrochemical characterization demonstrated that BG electrodes exhibit higher reversibility and electron transfer constant than the G electrode. All these results prove that PLD method is a viable alternative way to produce BG films with excellent electrochemical properties.

Published
2021
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2. Highly sensitive detection of Campylobacter spp. In chicken meat using a silica nanoparticle enhanced dot blot DNA biosensor

Author

Carole Chaix, Marisa Manzano, Jasmina Vidic, Priya Vizzini, Thierry Meylheuc, Nalini Ramarao, Carole Farre, MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), The authors thank the Centre Technologique des Microstructures of the Lyon 1 University for providing access to its TEM facilities, and the MIMA2 platform Jouy en Josas for access to electron microscopy equipment (MIMA2, INRAE, 2018. Microscopy and Imaging Facility for Microbes, Animals and Foods, https://doi.org/10.15454/1.5572348210007727E12).JV thanks Maria-Vesna Nikolic (University of Belgrade, Serbia) for English editing. PV acknowledges a doctoral fellowship from the University of Udine, Italy. This research was supported in part by the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 872662 (IPANEMA), the European Union's Horizon 2020 research and by the University Paris-Saclay through the Poc in labs 2019 grant agreement No 00003469 (OSCAR)., European Project: 872662 ,IPANEMA, Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)

Subjects
Campylobacter, DNA dot Blot, Food safety, Multiplex bacterial detection, Si-nanoparticles, Meat, DNA polymerase, Biomedical Engineering, Biophysics, Dot blot, Food Contamination, Biosensing Techniques, 02 engineering and technology, 01 natural sciences, Horseradish peroxidase, Luminol, chemistry.chemical_compound, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Electrochemistry, Animals, [CHIM]Chemical Sciences, Multiplex, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Chromatography, biology, Chemistry, 010401 analytical chemistry, technology, industry, and agriculture, DNA, Hydrogen Peroxide, General Medicine, Silicon Dioxide, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Blot, Biotinylation, biology.protein, Nanoparticles, Campylobacter, DNA dot Blot, Si-nanoparticles, Food safety, Multiplex bacterial detection, 0210 nano-technology, Chickens, Biosensor, Biotechnology
Abstract

The authors thank the Centre Technologique des Microstructures of the Lyon 1 University for providing access to its TEM facilities, and the MIMA2 platform Jouy en Josas for access to electron microscopy equipment (MIMA2, INRAE, 2018. Microscopy and Imaging Facility for Microbes, Animals and Foods, https://doi.org/10.15454/1. 5572348210007727E12). JV thanks Maria-Vesna Nikolic (University of Belgrade, Serbia) for English editing.; International audience; Paper-based DNA biosensors are powerful tools in point-of-care diagnostics since they are affordable, portable, user-friendly, rapid and robust. However, their sensitivity is not always as high as required to enable DNA quantification. To improve the response of standard dot blots, we have applied a new enhancement strategy that increases the sensitivity of assays based on the use of biotinylated silica-nanoparticles (biotin-Si-NPs). After immobilization of a genomic Campylobacter DNA onto a paper membrane, and addition of a biotinylated-DNA detection probe, hybridization was evidenced using streptavidin-conjugated to horseradish peroxidase (HRP) in the presence of luminol and H2O2. Replacement of the single biotin by the biotin-Si-NPs boosted on average a 30 fold chemiluminescent read-out of the biosensor. Characterization of biotin-Si-NPs onto a paper with immobilized DNA was done using a scanning electron microscope. A limit of detection of 3 pg/μL of DNA, similar to the available qPCR kits, is achieved, but it is cheaper, easier and avoids inhibition of DNA polymerase by molecules from the food matrices. We demonstrated that the new dot blot coupled to biotin-Si-NPs successfully detected Campylobacter from naturally contaminated chicken meat, without needing a PCR step. Hence, such an enhanced dot blot paves the path to the development of a portable and multiplex paper based platform for point-of-care screening of chicken carcasses for Campylobacter.

Published
2021
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3. Self-organized 3D graphene as a robust sensing platform

Author

Bourquand, F., Donnet, C., Florence GARRELIE, S Loir, A., Francis Vocanson, Carole Chaix, Carole Farre, Nicole Jaffrezic-Renault, Florence Lagarde, Gaëtan Raimondi, Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), in Palys Barbara (Ed), and Bussy, Agnès

Subjects
[CHIM] Chemical Sciences, [CHIM]Chemical Sciences, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2019

4. Combining culture and microbead-based immunoassay for the early and generic detection of bacteria in platelet concentrates

Author

Jeannette Fareh, Typhaine Dagland, Ludivine Vossier, Stéphanie Roche, Agnès Pouzet, Fanny Leon, Carole Farre, Christine Favier, Laetitia Rubrecht, Chantal Fournier-Wirth, Stéphane Rihet, Carole Chaix, Romaric Bonnet, Nicole Jaffrezic-Renault, Lionel Valera, Pascale Galea, Gaelle‐Anne Cremer, Dominique Piquer, Laboratoire TransDiag, Etablissement Français du Sang, Pathogénèse et contrôle des infections chroniques (PCCI), Centre Hospitalier Universitaire de Montpellier (CHU Montpellier )-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Sysdag, Sysdiag CNRS Bio-Rad UMR 3145, Cap Delta/Parc Euromédecine, Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), BIO-RAD, Sysdiag-Modélisation et Ingénierie des Systèmes Complexes Biologiques pour le Diagnostic (SysDiag ), BIO-RAD-Centre National de la Recherche Scientifique (CNRS), Systèmes d'élevage, nutrition animale et humaine (SENAH), AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA), Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), Centre National de la Recherche Scientifique (CNRS)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-École normale supérieure - Lyon (ENS Lyon)-Centre National de la Recherche Scientifique (CNRS)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-École normale supérieure - Lyon (ENS Lyon), Département de Chimie Moléculaire (DCM), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Universitaire de Montpellier (CHU Montpellier )-Université de Montpellier (UM), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])

Subjects
Acinetobacter baumannii, Blood Platelets, Microbiological culture, Immunology, 030204 cardiovascular system & hematology, Bacterial growth, Microbiology, 03 medical and health sciences, 0302 clinical medicine, medicine, Escherichia coli, Immunology and Allergy, Humans, Platelet, Serratia marcescens, ComputingMilieux_MISCELLANEOUS, Immunoassay, biology, medicine.diagnostic_test, Bacteria, Chemistry, Klebsiella oxytoca, Antibodies, Monoclonal, Hematology, Microbead (research), biology.organism_classification, Antibody production, Pseudomonas aeruginosa, biology.protein, Antibody, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, 030215 immunology
Abstract

BACKGROUND Despite current preventive strategies, bacterial contamination of platelets is the highest residual infectious risk in transfusion. Bacteria can grow from an initial concentration of 0.03-0.3 colony-forming units (CFUs)/mL up to 108 to 109 CFUs/mL over the product shelf life. The aim of this study was to develop a cost-effective approach for an early, rapid, sensitive, and generic detection of bacteria in platelet concentrates. STUDY DESIGN AND METHODS A large panel of bacteria involved in transfusion reactions, including clinical isolates and reference strains, was established. Sampling was performed 24 hours after platelet spiking. After an optimized culture step for increasing bacterial growth, a microbead-based immunoassay allowed the generic detection of bacteria. Antibody production and immunoassay development took place exclusively with bacteria spiked in fresh platelet concentrates to improve the specificity of the test. RESULTS Antibodies for the generic detection of either gram-negative or gram-positive bacteria were selected for the microbead-based immunoassay. Our approach, combining the improved culture step with the immunoassay, allowed sensitive detection of 1 to 10 CFUs/mL for gram-negative and 1 to 102 CFUs/mL for gram-positive species. CONCLUSION In this study, a new approach combining bacterial culture with immunoassay was developed for the generic and sensitive detection of bacteria in platelet concentrates. This efficient and easily automatable approach allows tested platelets to be used on Day 2 after collection and could represent an alternative strategy for reducing the risk of transfusion-transmitted bacterial infections. This strategy could be adapted for the detection of bacteria in other cellular products.

Published
2019
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5. Biomimetic vesicles for electrochemical sensing

Author

Carole Chaix, Carole Farre, Yves Chevalier, Catherine Jose, Estelle Lebègue, Florence Lagarde, Nicole Jaffrezic-Renault, Joëlle Saulnier, Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes-Centre National de la Recherche Scientifique (CNRS), Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes (2011-2014), Laboratoire d'automatique et de génie des procédés (LAGEP), Université de Lyon-Université de Lyon-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), Sciences Analytiques (SA), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Laboratoire d'automatique, de génie des procédés et de génie pharmaceutique (LAGEPP), EC, Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Université de Lyon-Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Materials science, Vesicle, 010401 analytical chemistry, Nanotechnology, 02 engineering and technology, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, Key issues, 01 natural sciences, 0104 chemical sciences, Analytical Chemistry, [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, Membrane, [CHIM.POLY]Chemical Sciences/Polymers, [SDV.SP.PG]Life Sciences [q-bio]/Pharmaceutical sciences/Galenic pharmacology, Microfluidic chip, Electrochemistry, Electrochemical biosensor, 0210 nano-technology, Biosensor, ComputingMilieux_MISCELLANEOUS
Abstract

International audience; Biomimetic vesicles, mainly composed of self-assembled bilayers of phospholipids, have attracted great attention for applications in the biosensor field over a number of decades, as a means to amplify the signal through encapsulated signal probes. In this review paper the most important developments in biomimetic vesicles for electrochemical biosensing within the last 2 years are presented, with a focus on the format of bioassays, their inclusion in microfluidic chip devices and their use in mimicking cell membranes. Key issues and the remaining challenges for future commercialization are analyzed.

Published
2018
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6. Responsive Polydiacetylene Vesicles for Biosensing Microorganisms

Author

Estelle Lebègue, Carole Farre, Yves Chevalier, Nicole Jaffrezic-Renault, Joëlle Saulnier, Carole Chaix, Florence Lagarde, Catherine Jose, Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'automatique, de génie des procédés et de génie pharmaceutique (LAGEPP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), EC, European Commission through a Marie Skłodowska Curie Individual Fellowship, Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes (2011-2014), Laboratoire d'automatique et de génie des procédés (LAGEP), and Sciences Analytiques (SA)

Subjects
analytical_chemistry, Polymers, Microorganism, Review, Biosensing Techniques, 02 engineering and technology, lcsh:Chemical technology, 010402 general chemistry, 01 natural sciences, Biochemistry, Analytical Chemistry, chemistry.chemical_compound, Toxins, lcsh:TP1-1185, Vesicles, Electrical and Electronic Engineering, Instrumentation, ComputingMilieux_MISCELLANEOUS, biology, Bacteria, Biosensing, Vesicle, Polyynes, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, biology.organism_classification, Fluorescence, Polyacetylene Polymer, Atomic and Molecular Physics, and Optics, 0104 chemical sciences, Virus, [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, [CHIM.POLY]Chemical Sciences/Polymers, [SDV.SP.PG]Life Sciences [q-bio]/Pharmaceutical sciences/Galenic pharmacology, chemistry, Biophysics, Colorimetry, Polydiacetylene, 0210 nano-technology, Peptides, Biosensor, DNA
Abstract

International audience; Polydiacetylene (PDA) inserted in films or in vesicles has received increasing attention due to its property to undergo a blue-to-red colorimetric transition along with a change from non-fluorescent to fluorescent upon application of various stimuli. In this review paper, the principle for the detection of various microorganisms (bacteria, directly detected or detected through the emitted toxins or through their DNA, and viruses) and of antibacterial and antiviral peptides based on these responsive PDA vesicles are detailed. The analytical performances obtained, when vesicles are in suspension or immobilized, are given and compared to those of the responsive vesicles mainly based on the vesicle encapsulation method. Many future challenges are then discussed

Published
2018
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7. Boron-doped Diamond Electrodes Modified with Fe 3 O 4 @Au Magnetic Nanocomposites as Sensitive Platform for Detection of a Cancer Biomarker, Interleukin-8

Author

Abdelhamid Errachid, Abdoullatif Baraket, Yi Yang, Aidong Zhang, Mohamed Braiek, Carole Farre, Carole Chaix, François Bessueille, Nicole Jaffrezic-Renault, Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Key Lab Pesticide & Chem Biol, Central China Normal University [Wuhan, China], Surfaces, Micro & Nanobiotechnologies, The authors thank the EC through the Marie Curie NANODEV Grant No318524 for financial support. We acknowledge the funding through the European Communities Seventh Framework Programme entitled Sea-on-a-Chip (FP7-OCEAN-2013) (No.614168) and the European Union's Horzion 2020 Research and Innovation Programme entitled HEARTEN under grant agreement No.643694., European Project: 643694,H2020,H2020-PHC-2014-single-stage,HEARTEN(2015), European Project: 614168,EC:FP7:KBBE,FP7-OCEAN-2013,SEA-ON-A-CHIP(2013), European Project: 318524,EC:FP7:PEOPLE,FP7-PEOPLE-2012-IRSES,NANODEV(2013), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Detection limit, Nanocomposite, Analytical chemistry, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, Electrochemistry, 01 natural sciences, 0104 chemical sciences, Analytical Chemistry, Dielectric spectroscopy, chemistry.chemical_compound, chemistry, Colloidal gold, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Magnetic nanoparticles, Ferricyanide, Cyclic voltammetry, 0210 nano-technology, Nuclear chemistry
Abstract

International audience; Interleukin-8 (IL-8), a dimeric protein composed of two identical subunits, is overexpressed in different human cancers, among them pancreatic cancers and head and neck cancers. There is a strong demand for monitoring of this biomarker protein for early cancer detection. A new concept of label-free impedimetric immunosensors for IL-8 detection is described in this work. A nanocomposite of gold nanoparticles (AuNPs) decorating a magnetic Fe3O4 core is synthesized using cysteamine as linker, and characterized using TEM and UV-Vis spectrometry. After immobilization of these Fe3O4@Au magnetic nanocomposites on a boron doped diamond (BDD) electrode using a simple magnet, anti-human IL-8 monoclonal antibody is linked to the AuNPs, previously functionalized with thioctic acid. The functionalization of the immunosensor was characterized using two electrochemical techniques: cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), performed in phosphate buffer with ferro/ferricyanide as the redox probe. The EIS technique was used for affinity assays: antibody-Antigen binding. A linear relationship between the increment in the electron transfer resistance (R-CT) and the logarithmic value of IL-8 concentration was observed between 0.1pg/mL and 1000pg/mL. The limit of detection (LOD) was estimated at 0.03pg/mL.

Published
2016
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8. Effect of Perfluorinated-Hexaethylene Glycol Functionalization of Gold Nanoparticles on the Enhancement of the Response of an Enzymatic Conductometric Biosensor for Urea Detection

Author

Carole Farre, Nicole Jaffrezic-Renault, Mohamed Braiek, Mengjuan Jiang, Anne Bonhomme, Carole Chaix, Aidong Zhang, Jean-François Chateaux, Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), spectroscopies vibrationnelles, INL - Lab-On-Chip et Instrumentation (INL - LOCI), Institut des Nanotechnologies de Lyon (INL), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Lyon (ECL), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Key Lab Pesticides & Chem Biol, Central China Normal University [Wuhan, China], and Bussy, Agnès

Subjects
[CHIM.ANAL] Chemical Sciences/Analytical chemistry, Materials science, Urease, Analytical chemistry, Nanoparticle, 02 engineering and technology, 010501 environmental sciences, 01 natural sciences, chemistry.chemical_compound, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Spectrophotometry, medicine, 0105 earth and related environmental sciences, Detection limit, biology, medicine.diagnostic_test, General Medicine, 021001 nanoscience & nanotechnology, chemistry, Colloidal gold, biology.protein, Urea, Surface modification, 0210 nano-technology, Biosensor, Nuclear chemistry
Abstract

International audience; In conductometric enzymatic biosensors, enzymatic reaction is confined close to the interdigitated electrode surface, because enzyme is cross-linked in contact with this surface in the presence or absence of nanoparticles. The effect of the use of a new type of doubly-functionalized gold nanoparticles (PF-HEG-Au NPs) on the response of conductometric biosensor based on interdigitated electrodes (IDEs), for the detection of enzymatic substrates was studied. Gold nanoparticles (AuNPs) were first synthesized following the citrate process, with an average diameter of 14 nm. AuNPs were then functionalized with 11-mercaptoundecylhexaethyleneglycol (HEG) and then with 1H,1H,2H,2H-perfluorodecanethiol (PF). The doubly-functionalized AuNPs were characterized using TEM, UV-Vis spectrophotometry and FTIR spectroscopy. Urease, mixed with these doubly functionalized AuNPs, was then cross-linked with glutaraldhedyde vapor on the IDE surface. In the presence of urea, the conductometric response was measured in a differential mode. The best sensitivities for urea detection were obtained with PF-HEG-Au NPs (520 µS /mM and 0.5µM of detection limit), as compared to 284µS/mM and 2µM of detection limit with bare Au NPs, PF-AuNPs and HEG-AuNPs, and 1.07µS/mM and 100 µM of detection limit with urease directly crosslinked on IDEs.When stored in phosphate buffer (5 mM, pH 6.7) at 4 °C, the biosensor with PF-HEG-Au NPs showed good stability for more than 12 days.

Published
2016

9. Molecularly imprinted polymer-based electrochemical sensor for the sensitive detection of glyphosate herbicide

Author

Minh Huy Do, Anca Florea, Francis Vocanson, Tran-Thi Nhu-Trang, François Bessueille, Carole Farre, Nicole Jaffrezic-Renault, Anne Bonhomme, Dept Analyt Chem, VNUHCM Univ Sci, Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Surfaces, Laboratoire Hubert Curien [Saint Etienne] (LHC), Institut d'Optique Graduate School (IOGS)-Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS), and This work was supported by European Social Fund, Human Resources Development Operational Programme 2007-2013 [project number POSDRU/159/1.5/S/136893].

Subjects
EXTRACTION, animal structures, Health, Toxicology and Mutagenesis, GAS-CHROMATOGRAPHY, Soil Science, 02 engineering and technology, 01 natural sciences, metal organic framework, Analytical Chemistry, chemistry.chemical_compound, Aniline, glyphosate, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, METAL-ORGANIC FRAMEWORK, Environmental Chemistry, Molecule, WATER, Electrochemical sensors, Aminomethylphosphonic acid, Waste Management and Disposal, Water Science and Technology, Chromatography, FLUORESCENCE DETECTION, integumentary system, 010401 analytical chemistry, Public Health, Environmental and Occupational Health, Molecularly imprinted polymer, MASS-SPECTROMETRY, 021001 nanoscience & nanotechnology, Pollution, Combinatorial chemistry, PERFORMANCE LIQUID-CHROMATOGRAPHY, 0104 chemical sciences, Electrochemical gas sensor, chemistry, Colloidal gold, gold nanoparticles, Linear sweep voltammetry, embryonic structures, RESIDUES, molecularly imprinted polymers, AMINOMETHYLPHOSPHONIC ACID, 0210 nano-technology, Selectivity
Abstract

International audience; A sensitive electrochemical molecularly imprinted sensor was developed for the detection of glyphosate (Gly), by electropolymerisation of p-aminothiophenol-functionalised gold nanoparticles in the presence of Gly as template molecule. The extraction of the template leads to the formation of cavities that are able to specifically recognise and bind Gly through hydrogen bonds between Gly molecules and aniline moieties. The performance of the developed sensor for the detection of Gly was investigated by linear sweep voltammetry using a hexacyanoferrate/hexacyanoferrite solution as redox probe, the electron transfer rate increasing when concentration of Gly increases, due to a p-doping effect. The molecularly imprinted sensor exhibits a broad linear range, between 1 pg/L and 1 mu g/L and a quantification limit of 0.8 pg/L. The selectivity of the proposed sensor was investigated towards the binding of Gly metabolite, aminomethylphosphonic acid, revealing excellent selectivity towards Gly. The developed sensor was successfully applied to detect Gly in tap water samples.

Published
2015
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10. Methylene blue phosphoramidite for DNA labelling

Author

Monique Sigaud, Corinne Sanglar, Gabriel De Crozals, Philippe Fortgang, Carole Chaix, Carole Farre, Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Micro & Nanobiotechnologies, and Lyon Science Transfert-Universite de Lyon and the French Ministry of Research for their financial support.

Subjects
SALTS, Photochemistry, SEQUENCE, Catalysis, OLIGONUCLEOTIDES, chemistry.chemical_compound, Electron transfer, PHOTODYNAMIC THERAPY, Organophosphorus Compounds, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Labelling, Materials Chemistry, Molecule, ELECTRON-TRANSFER, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Solid-Phase Synthesis Techniques, Phosphoramidite, ELECTROCHEMICAL HYBRIDIZATION INDICATOR, Oligonucleotide, Metals and Alloys, SENSOR, Electrochemical Techniques, General Chemistry, GOLD ELECTRODES, Combinatorial chemistry, 3. Good health, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Methylene Blue, chemistry, Ceramics and Composites, PHENOTHIAZINIUM PHOTOSENSITIZERS, Derivative (chemistry), Methylene blue, DNA, STEM-LOOP PROBE
Abstract

We thank Oceane Clabaux and Alexandre Escoffier for their contribution to the work.; International audience; We report the first synthesis of a methylene blue (MB) phosphoramidite derivative suitable for DNA solid-phase synthesis. The electrochemical and optical properties of the resulting MB modified oligonucleotides were confirmed. This new molecule is an important breakthrough in the design of new probes labelled with MB.

Published
2015
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11. One-Step Fabrication of Electrospun Photo-Cross-Linkable Polymer Nanofibers Incorporating Multiwall Carbon Nanotubes and Enzyme for Biosensing

Author

Madjid Arab, Carole Farre, Mohamed Braiek, Florence Lagarde, Eleni Sapountzi, Jean-François Chateaux, Nicole Jaffrezic-Renault, Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Interfaces et Matériaux Avancés [Monastir] (LIMA), Faculté des Sciences de Monastir (FSM), Université de Monastir - University of Monastir (UM)-Université de Monastir - University of Monastir (UM), Institut des Matériaux, de Microélectronique et des Nanosciences de Provence (IM2NP), Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), INL - Lab-On-Chip et Instrumentation (INL - LOCI), Institut des Nanotechnologies de Lyon (INL), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Lyon (ECL), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), the authors thank the French government for the financial support., Aix Marseille Université (AMU)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Lyon (ECL), and Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
COMPOSITE NANOFIBERS, GLUCOSE-OXIDASE, Materials science, POLY(VINYL ALCOHOL), Nanotechnology, Carbon nanotube, MEMBRANES, Polyvinyl alcohol, law.invention, chemistry.chemical_compound, DISPERSION, law, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Materials Chemistry, Electrochemistry, SENSORS, Glucose oxidase, biology, Renewable Energy, Sustainability and the Environment, MECHANICAL-PROPERTIES, [CHIM.MATE]Chemical Sciences/Material chemistry, PERFORMANCE, Condensed Matter Physics, Electrospinning, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Dielectric spectroscopy, Chemical engineering, chemistry, IMMOBILIZATION, Nanofiber, biology.protein, Cyclic voltammetry, Biosensor, FIBERS
Abstract

The authors thank INL institute (Institut des Nanotechnologies de Lyon, France) for providing the facilities for the electrospinning technique, the required access to the SEM facilities and for their remarkable assistance whenever it was needed. The Centre Technologique des Microstructures of Lyon 1 University is also acknowledged for providing the authors access to its TEM facilities. Finally, the authors thank the French government for the financial support.; International audience; Herein, we report the easy and rapid fabrication of water-stable electrospun nanofibers from blends of the photochemically cross-linkable polyvinyl alcohol styrylpyridinium polymer (PVA-SbQ), carboxylated multiwall carbon nanotubes (MWCNT-COOHs) and gluccise oxidase (GOx) for electrochemical biosensor application. Different electrospinning parameters including flow rate, applied voltage, distance between tip and collector, polymer concentration and MWCNT-COOHs content in the electrospun solution were tailored to produce PVA-SbQ/MWCNT-COOH nanofibers with minimal beading and enhanced electrical properties. Optimal PVA-SbQ and MWCNT-COOHs concentrations were 6.7 wt% and 5 wt% with respect to the total solution and polymer mass, respectively. The nanofibers were rendered water insoluble via exposure to UV irradiation for 10 mm. The electrochemical properties of electrospun PVA-SbQ/MWCNT-COOH nanofibrous mats were characterized by cyclic voltammetry and electrochemical impedance spectroscopy. Scanning electron microscopy and transmission electron microscopy were used to characterize the morphology of the nanofibers. G0x, used as model enzyme, was further incorporated into the PVA-SbQ/MWCNT-COOH mixture before electrospinning. The obtained biosensor enabled successful detection of glucose by cyclic voltammetry. The resulting novel glucose biosensor revealed very good storage and operational stabilities, a low limit of detection (2 mu M) and a wide linear range (up to at least 4 mM).

Published
2015
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12. Nanoparticules fluorescentes ciblantes pour l'imagerie médicale

Author

Grégoire Hantier, Gabriel de Crozals, Carole Farre, Bonazza, P., Claire Billotey, Marc Janier, Jean-Jacques Toulmé, Marquette, Christophe A., Cédric Louis, Carole Chaix, SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes (2011-2014), Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physico-Chimie des Matériaux Luminescents (LPCML), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Imagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie (IECB), Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Nano-H SAS, SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes, Institut des Sciences Analytiques ( ISA ), École normale supérieure - Lyon ( ENS Lyon ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique ( CNRS ) -École normale supérieure - Lyon ( ENS Lyon ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de Physico-Chimie des Matériaux Luminescents ( LPCML ), Université Claude Bernard Lyon 1 ( UCBL ), Imagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie ( IECB ), Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique ( CNRS ), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires ( ICBMS ), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon ( INSA Lyon ), Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique ( CNRS ), and Bussy, Agnès

Subjects
[CHIM.ANAL] Chemical Sciences/Analytical chemistry, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, [ CHIM.ANAL ] Chemical Sciences/Analytical chemistry, ComputingMilieux_MISCELLANEOUS
Abstract

communication par affiche; National audience

Published
2012

13. Oligonucleotide solid-phase synthesis on fluorescent silica particles of nanometric size

Author

Gabriel de Crozals, Carole Farre, Marquette, Christophe A., Mandon, Céline A., Matteo Martini, Jean-Jacques Toulmé, Darocha, S., Claire Billotey, Marc Janier, Laurence Marmuse, Cédric Louis, Carole Chaix, SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes, Institut des Sciences Analytiques ( ISA ), Centre National de la Recherche Scientifique ( CNRS ) -Université de Lyon-Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-École normale supérieure - Lyon ( ENS Lyon ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Lyon-Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-École normale supérieure - Lyon ( ENS Lyon ), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires ( ICBMS ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon ( INSA Lyon ), Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de Physico-Chimie des Matériaux Luminescents ( LPCML ), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique ( CNRS ), Imagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie ( IECB ), Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique ( CNRS ), Nano-H SAS, Bussy, Agnès, SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes (2011-2014), Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physico-Chimie des Matériaux Luminescents (LPCML), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Imagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie (IECB), and Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique (CNRS)

Subjects
[CHIM.ANAL] Chemical Sciences/Analytical chemistry, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, [ CHIM.ANAL ] Chemical Sciences/Analytical chemistry
Published
2012

14. A sensitive and selective thrombin impedimetric aptasensor based on tailored aptamers obtained by solid-phase synthesis

Author

R. Kherrat, Nicole Jaffrezic-Renault, N. Meini, Sergei V. Dzyadevych, Carole Chaix, Carole Farre, Laboratoire Génie de l'Environnement, Université Badji Mokhtar - Annaba [Annaba] (UBMA), SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes (2011-2014), Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratory of Biomolecular Electrochemistry, National Academy of Sciences of Ukraine (NASU), AUF ( PhD thesis grant), EC (NANOBIOSENS Project PIRSES-GA-2008-2300802), NATO project CBP.NUKR.CLG 984221, European Project, Université Badji Mokhtar Annaba (UBMA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Aptamer, PROTEINS, Phosphoramidite synthons, Nanotechnology, 02 engineering and technology, 01 natural sciences, Solid-phase synthesis, Thrombin, BIOSENSOR, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Materials Chemistry, medicine, SURFACE-PLASMON RESONANCE, ASSAY, Electrical and Electronic Engineering, Surface plasmon resonance, Instrumentation, Detection limit, Phosphoramidite, Chemistry, 010401 analytical chemistry, Metals and Alloys, Aptasensor, DNA, 021001 nanoscience & nanotechnology, Condensed Matter Physics, 0104 chemical sciences, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Dielectric spectroscopy, IMPEDANCE SPECTROSCOPY DETECTION, LIGANDS, 0210 nano-technology, Biosensor, Electrochemical impedance spectroscopy, medicine.drug
Abstract

International audience; The aim of the present work is to show the interest of a generic concept of aptamers directly obtained through a supported synthesis, using phosphoramidite synthons, for biosensor application. By that way, we were able to tailor the anchoring groups and specific spacers. The concept is validated on an impedimetric aptasensor for detection of thrombin. Two aptamer sequences, selected from literature (15 and 29 mers), were synthesized with a multi-thiol modification at one extremity. After their grafting on gold electrode, the faradic electrochemical impedance measurements allow the direct detection of thrombin in the range of 3-30 ng mL(-1) ancl 3-50 ng mL(- 1) for 15 meraptamer and 29 meraptamer respectively, with a detection limit of 3.1 ng mL(-1) (80 pmol/l) for both sequences. This study demonstrates that these aptasensors exhibit high sensitivity (higher sensitivity for 29 meraptamer), high selectivity (compared to a model protein, BSA), stability and reproducibility

Published
2012
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15. Oligonucleotide solid-phase synthesis on fluorescent nanoparticles grafted on controlled pore glass

Author

Carole Chaix, Carole Farre, Grégoire Hantier, Jean-Jacques Toulmé, Marc Janier, Claire Billotey, Didier Léonard, Christophe A. Marquette, Cédric Louis, Gabriel De Crozals, Laurence Marmuse, Céline A. Mandon, SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes, Institut des Sciences Analytiques ( ISA ), École normale supérieure - Lyon ( ENS Lyon ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique ( CNRS ) -École normale supérieure - Lyon ( ENS Lyon ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique ( CNRS ), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires ( ICBMS ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon ( INSA Lyon ), Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique ( CNRS ), Nano-H SAS, Imagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie ( IECB ), Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de Physico-Chimie des Matériaux Luminescents ( LPCML ), ANR (ANR-08-Biotecs-003), LyonBiopole, Lyon Science Transfert, SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes (2011-2014), Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Imagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie (IECB), Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physico-Chimie des Matériaux Luminescents (LPCML), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon

Subjects
Materials science, General Chemical Engineering, Nanoparticle, Nanotechnology, BIOANALYSIS, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, chemistry.chemical_compound, Solid-phase synthesis, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, OXIDE NANOPARTICLES, Phosphoramidite, DNA synthesis, Oligonucleotide, General Chemistry, DNA, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Chemical engineering, chemistry, IMMOBILIZATION, Colloidal gold, GOLD NANOPARTICLES, Surface modification, FUNCTIONALIZATION, [ CHIM.ANAL ] Chemical Sciences/Analytical chemistry, 0210 nano-technology
Abstract

International audience; Oligonucleotide solid-phase synthesis is now possible on nano-sized particles, thanks to the use of controlled pore glass-nanoparticle assemblies. We succeeded in anchoring silica nanoparticles (NPs) inside the pores of micrometric glass via a reversible covalent binding. The pore diameter must be at least six times the diameter of the nanoparticle in order to maintain efficient synthesis of oligonucleotides in the synthesizer. We demonstrated that the pores protect NP anchoring during DNA synthesis without decreasing the coupling rate of the phosphoramidite synthons. This bottom-up strategy for NP functionalization with DNA results in unprecedented DNA loading efficiency. We also confirmed that the DNA synthesized on the nanoparticle surface was accessible for hybridization with its complementary DNA strand.

Published
2012
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16. A 'four-ferrocene' modified stem-loop structure as a probe for sensitive detection and single-base mismatch discrimination of DNA

Author

Carole Chaix, Carole Farre, Grégory Chatelain, Salomé Ansanay-Alex, Micaël Ripert, SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes (2011-2014), Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)

Subjects
Detection limit, Electrochemical transduction, 010405 organic chemistry, Oligonucleotide, General Chemical Engineering, Analytical chemistry, DNA, 010402 general chemistry, 01 natural sciences, Genosensor, 0104 chemical sciences, chemistry.chemical_compound, Ferrocene, chemistry, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Electrode, Electrochemistry, Stem-loop structure, Differential pulse voltammetry, Cyclic voltammetry, Biochip, Biosensor
Abstract

International audience; We report the use of a four-ferrocene modified oligonucleotide as a probe for DNA detection with a gold electrode microsystem. This oligonucleotide is synthesized by automated solid-phase synthesis with four successive ferrocene moieties at the 5′-end and a C6-thiol modifier group at the 3′-end. The grafting of this 4Fc-DNA probe on a gold electrode microsystem results in the appearance of the ferrocene redox couple in cyclic voltammetry. The probe sequence is a stem-loop structure that folds efficiently on the electrode, thus optimizing electron transfer. Such architecture serves as sensor for DNA detection which is based on hybridization. The resulting disposable voltammetric sensor allowed direct, reagentless DNA detection in a small volume (20 μL). Electrochemical response upon hybridization with complementary short sequence (30-base length) and long sequence (50-base length) strands was observed by differential pulse voltammetry. Current variations were compared. The longer the sequence, the greater the decrease in current. The system's detection limit was estimated at 3.5 pM (0.07 fmol in 20 μL) with the 50-base length target and provided a dynamic detection range between 3.5 pM and 5 nM. Single mismatch detection showed a good level of sensitivity. The system was regenerated twice with no significant loss of Fc signal. Finally, 1 pM sensitivity was reached with a long chain analog of DNA PCR products of Influenza virus.

Published
2012
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17. Impedimetric aptasensor for thrombin detection

Author

Nicole Jaffrezic-Renault, N. Meini, Carole Farre, K. Kherrat, Sergei V. Dzyadevych, Carole Chaix, Université Badji Mokhtar - Annaba [Annaba] (UBMA), SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes (2011-2014), Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratory of Biomolecular Electrochemistry, and National Academy of Sciences of Ukraine (NASU)

Subjects
Aptamer, High selectivity, Analytical chemistry, 02 engineering and technology, 01 natural sciences, Thrombin, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, medicine, aptasensor, Engineering(all), Detection limit, Reproducibility, Chromatography, Chemistry, 010401 analytical chemistry, Model protein, aptamer, electrochemical impedance spectroscopy (EIS), General Medicine, 021001 nanoscience & nanotechnology, thrombin, 0104 chemical sciences, Electrode, 0210 nano-technology, [CHIM.OTHE]Chemical Sciences/Other, medicine.drug
Abstract

International audience; The goal of this work is to develop an impedimetric aptasensor sensitive, selective and stable for detection of thrombin. Thiol modified aptamer sequence being grafted on gold electrode, the faradic electrochemical impedance measurements allow the direct detection of thrombin in the range of 3.1-200 ngmL(-1) with a detection limit of 3.1 ngmL(-1) (80 fM). This study demonstrates that these aptasensors exhibit high sensitivity, high selectivity (compared to a model protein, BSA), stability and reproducibility.

Published
2011
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18. Automated oligonucleotide solid-phase synthesis on nanosized silica particles using nano-on-micro assembled particle supports

Author

Gaëlle Catanante, Stéphane Roux, Christophe A. Marquette, Cédric Louis, Carole Chaix, Loïc J. Blum, Carole Farre, Muriel Lansalot, Nicolas Charvet, Rana Bazzi, Laboratoire de chimie et procédés de polymérisation (LCPP), École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), Département Economie et Sociologie des Transports (INRETS/DEST), Institut National de Recherche sur les Transports et leur Sécurité (INRETS), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institute of Molecular Biology and Biotechnology (IMBB-FORTH), Foundation for Research and Technology - Hellas (FORTH), Department of Biology, University of Crete [Heraklion] (UOC), Sciences Analytiques (SA), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), and Depierre, Frédérique

Subjects
Scanning electron microscope, Oligonucleotides, Binary compound, Nanoparticle, Nanotechnology, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, chemistry.chemical_compound, Solid-phase synthesis, Microscopy, Electron, Transmission, Nano, Scanning transmission electron microscopy, Electrochemistry, General Materials Science, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Spectroscopy, Surfaces and Interfaces, Models, Theoretical, respiratory system, 021001 nanoscience & nanotechnology, Condensed Matter Physics, Silicon Dioxide, 0104 chemical sciences, chemistry, Oligodeoxyribonucleotides, Transmission electron microscopy, Microscopy, Electron, Scanning, Particle, Nanoparticles, 0210 nano-technology
Abstract

International audience; This article describes an original strategy to enable solid-phase oligodeoxyribonucleotide (ODN) synthesis on nanosized silica particles. It consists of the reversible immobilization of silica nanoparticles (NPs) on micrometric silica beads. The resulting assemblies, called nano-on-micro (NOM) systems, are well adapted to ODN synthesis in an automated instrument. First, NPs are derivatized with OH functions. For NOM assembly preparation, these functions react with the silanols of the microbeads under specific experimental conditions. Furthermore, OH groups allow ODN synthesis on the nanoparticles via phosphoramidite chemistry. The stability of the NOM assemblies during ODN solid-phase synthesis is confirmed by scanning and transmission electron microscopy (SEM and TEM, respectively), together with dynamic light scattering analyses. Then, the release of ODN-functionalized nanoparticles is performed under mild conditions (1% NH4OH in water, 1 h, 60 °C). Our technique provides silica nanoparticles well functionalized with oligonucleotides, as demonstrated by hybridization experiments conducted with the cDNA target.

Published
2010

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