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6 results on '"Baron, Christophe"'

1. THE VOLTAGE-GATED SODIUM CHANNEL BETA4 SUBUNIT MAINTAINS EPITHELIAL PHENOTYPE IN MAMMARY CELLS

Author

Doray, Adélaïde, Lemoine, Roxane, Severin, Marc, Chadet, Stéphanie, Lopez-Charcas, Osbaldo, Héraud, Audrey, Baron, Christophe, Besson, Pierre, Monteil, Arnaud, Pedersen, Stine Falsig, Roger, Sébastien, EA4245 - Transplantation, Immunologie, Inflammation [Tours] (T2i), Université de Tours (UT), University of Copenhagen = Københavns Universitet (KU), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), Université de Tours, University of Copenhagen = Københavns Universitet (UCPH), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), and Monteil, Arnaud

Subjects
QH301-705.5, [SDV]Life Sciences [q-bio], NaVβ4, Down-Regulation, Article, Cell Line, Mesoderm, Mammary Glands, Animal, Epithelial-to-Mesenchymal Transition, Animals, Humans, Biology (General), beta Catenin, Voltage-Gated Sodium Channel beta-4 Subunit, Cell Polarity, Epithelial Cells, β-catenin, [SDV] Life Sciences [q-bio], Protein Subunits, Phenotype, Proteolysis, Female, mammary cells Powered by Editorial Manager® and ProduXion Manager® from Aries Systems Corporation, epithelial-to-mesenchymal transition, mammary cells, epithelial phenotype
Abstract

The SCN4B gene, coding for the NaVβ4 subunit of voltage-gated sodium channels, was recently found to be expressed in normal epithelial cells and down-regulated in several cancers. However, its function in normal epithelial cells has not been characterized. In this study, we demonstrated that reducing NaVβ4 expression in MCF10A non-cancer mammary epithelial cells generated important morphological changes observed both in two-dimensional cultures and in three-dimensional cysts. Most notably, the loss of NaVβ4 induced a complete loss of epithelial organisation in cysts and increased proteolytic activity towards the extracellular matrix. Loss of epithelial morphology was associated with an increased degradation of β-catenin, reduced E-cadherin expression and induction of mesenchymal markers N-cadherin, vimentin, and α-SMA expression. Overall, our results suggest that Navβ4 may participate in the maintenance of the epithelial phenotype in mammary cells and that its downregulation might be a determining step in early carcinogenesis.

Published
2021

2. Rock inhibition promotes NaV1.5 sodium channel-dependent SW620 colon cancer cell invasiveness

Author

Poisson, Lucile, Lopez-Charcas, Osbaldo, Chadet, Stéphanie, Bon, Emeline, Lemoine, Roxane, Brisson, Lucie, Ouaissi, Mehdi, Baron, Christophe, Besson, Pierre, Roger, Sébastien, Moussata, Driffa, EA4245 - Transplantation, Immunologie, Inflammation [Tours] (T2i), Université de Tours (UT), Nutrition, croissance et cancer (U 1069) (N2C), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), This work was supported by the University of Tours, the 'Ligue Nationale Contre le Cancer – Interrégion Grand-Ouest' to DM and SR, the Région Centre-Val de Loire (Grant 'NaVMetarget') to SR, and the 'Association CANCEN'. S.R. was recipient of a prize 'Prix Ruban Rose Avenir 2017' from the Charity 'le Cancer du sein: parlons-en!'. LP was recipient of an 'Inserm-Region Centre-Val de Loire' PhD fellowship. OLC was recipient of a funding from the 'Fondation pour la Recherche Médicale (FRM)' (SPF201909009198)., besson, pierre, Université de Tours, Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM), and Bodescot, Myriam

Subjects
[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, [SDV.CAN] Life Sciences [q-bio]/Cancer, lcsh:R, lcsh:Medicine, lcsh:Q, [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, lcsh:Science, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, ComputingMilieux_MISCELLANEOUS
Abstract

International audience; The acquisition of invasive capacities by carcinoma cells, i.e. their ability to migrate through and to remodel extracellular matrices, is a determinant process leading to their dissemination and to the development of metastases. these cancer cell properties have often been associated with an increased Rho-ROCK signalling, and ROCK inhibitors have been proposed for anticancer therapies. In this study we used the selective ROCK inhibitor, Y-27632, to address the participation of the Rho-ROCK signalling pathway in the invasive properties of SW620 human colon cancer cells. Contrarily to initial assumptions, Y-27632 induced the acquisition of a pro-migratory cell phenotype and increased cancer cell invasiveness in both 3- and 2-dimensions assays. This effect was also obtained using the other ROCK inhibitor Fasudil as well as with knocking down the expression of ROCK-1 or ROCK-2, but was prevented by the inhibition of NaV1.5 voltage-gated sodium channel activity. Indeed, ROCK inhibition enhanced the activity of the pro-invasive NaV1.5 channel through a pathway that was independent of gene expression regulation. In conclusions, our evidence identifies voltage-gated sodium channels as new targets of the ROCK signalling pathway, as well as responsible for possible deleterious effects of the use of ROCK inhibitors in the treatment of cancers.

Published
2020

3. Les mécanismes de l’alloréactivité, des rejets de greffe et de la réaction du greffon contre l’hôte. Chapitre 27

Author

Blancho, Gilles, de Carvalho-Bittencourt, Marcelo, Baron, Christophe, Labalette, Myriam, Moins-Teisserenc, Hélène, Sterkers, Ghislaine, Tiberghien, Pierre, Centre hospitalier universitaire de Nantes (CHU Nantes), Nantes Université - UFR de Médecine et des Techniques Médicales (Nantes Univ - UFR MEDECINE), Nantes Université - pôle Santé, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ), Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Université de Tours (UT), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Université de Lille, Hopital Saint-Louis [AP-HP] (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), AP-HP Hôpital universitaire Robert-Debré [Paris], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC), and DE CARVALHO, MARCELO

Subjects
[SDV.IMM] Life Sciences [q-bio]/Immunology, [SDV.IMM]Life Sciences [q-bio]/Immunology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

4. Explanations for the good behaviour in sewer networks of calcium aluminate cement materials

Author

Herisson, Jean, Gueguen Minerbe, Marielle, Van Hullebusch, Eric D., EYCHENNE-BARON, Christophe, Chaussadent, Thierry, Comportement Physico-chimique et Durabilité des Matériaux (IFSTTAR/MAST/CPDM), Communauté Université Paris-Est-Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR), Laboratoire Géomatériaux et Environnement (LGE), Université Paris-Est Marne-la-Vallée (UPEM), Kerneos Research and Technology Center, Cadic, Ifsttar, and Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR)-Communauté Université Paris-Est

Subjects
[CHIM.MATE] Chemical Sciences/Material chemistry, RESEAU D'ASSAINISSEMENT, CORROSION, [CHIM.MATE]Chemical Sciences/Material chemistry, [SPI.MAT] Engineering Sciences [physics]/Materials, [SPI.MAT]Engineering Sciences [physics]/Materials
Abstract

Sewer networks are subjected to many corrosion agents leading to deterioration of variant magnitude. One type of damage can be ascribed to concrete corrosion by biogenic sulfuric acid. According to field data, cementitious materials have different behaviours depending more especially on cement type. Those made with calcium aluminate cement (CAC) offer better performance than those made of ordinary Portland cement (OPC). This difference of durability may have three origins that can be combined. The first one is due to the oxidation of hydrogen sulfide at the surface of each materials leading to a lower amount of elemental sulfur deposited on CAC materials due to the difference of matrix composition (chemical composition and mineralogy). Elemental sulfur is then oxidized into sulfuric acid by microorganisms and leads to cementitious materials corrosion. The second one, the alumina content of CAC materials stabilizes the surface pH at around 3-4 and therefore limits the development of the most deleterious sulfur oxidizing bacteria. The last one, the aluminium content of CAC has a bacteriostatic effect on some microorganisms, limiting biofilm development. All that results are participating at the good performances of CAC materials.

Published
2013

5. Sulfur cycle in sewer networks and its consequences on cementitious materials

Author

Herisson, Jean, Van Hullebusch, Eric D., Gueguen, Marielle, Moletta-Denat, Marina, EYCHENNE-BARON, Christophe, Chaussadent, Thierry, Cadic, Ifsttar, Laboratoire Géomatériaux et Environnement (LGE), Université Paris-Est Marne-la-Vallée (UPEM), Département Matériaux (IFSTTAR/MAT), Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR)-PRES Université Paris-Est, Laboratoire de Biotechnologie de l'Environnement [Narbonne] (LBE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA), Kerneos Research Technology Center, Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA), and Lge, Admin

Subjects
BETON, MICROORGANISME, ASSAINISSEMENT, BACTERIE, DEGRADATION, [SPI.MAT] Engineering Sciences [physics]/Materials, ALUMINATE, [SPI.MAT]Engineering Sciences [physics]/Materials
Abstract

Sewer networks enclose many biological and chemical transformations involving sulfur molecules oxidation leading to biogenic sulfuric acid production. Sewage effluents contain many sulfur species such as sulfates and sulfites which are reduced in anoxic zones by sulfate reducing bacteria (Desulfovibrio sp., Desulfobulbus sp.) into hydrogen sulfide (H2S). This gas is emitted in the aerial part of the pipe and degrades materials. This deterioration proceeds either directly as H2S is a corrosive gas, or indirectly through the action of microorganisms that will oxidize H2S into sulfuric acid. This acid will dissolve the cement matrix and produce expansive products (such as gypsum or ettringite). In the worst case it leads to pipes destruction. This study aims at understanding the deterioration mechanisms occuring in the aerial part of the pipe. A special attention is given to the impact of different cementitious material on microorganisms involved in the global sulfur cycle (sulfur oxidizing bacteria such as Starkeya novella, Halothiobacillus neapolitanus, Thiomonas intermedia and Acidithiobacillus thiooxidans). According to field data, cementitious materials made with Calcium Aluminate Cement (CAC) offer far better performance on site than those made of Ordinary Portland Cement (OPC). This behaviour difference is likely associated with a different microorganism diversity. This work is divided into three parts. The first one consists in an exposition of CAC and OPC mortars in real sewer networks. This on-site experiment aims to follow the evolution of biodiversity and also to characterize the degradation of cementitious materials. Capillary Electrophoresis - Single Strand Conformation Polymorphism (CE-SSCP) analyses performed on each sample reveals that biodiversity is highly related to cement composition. OPC mortars hosts more numerous and diverse microorganisms than CAC mortars. The second part is represented by various lab experiments aiming to better describe the different steps involved in the whole mechanism. According to our tests, abiotic oxidation of H2S into elemental sulfur is highly dependent on materials composition. While elemental sulfur deposition can be seen on OPC mortars, none is observed on CAC mortars. Depending on sulfur source available, bacteria will have various growth rates. We have studied the impact of three sulfur sources (S2O32-, S0, H2S) on the growth of sulfur oxidising bacteria. All results obtained are used, in a third part, to design an accelerated experiment that should be standardized to test sewer networks materials.

Published
2012

6. Reaction of Butyltin Hydroxide Oxide with p -Toluenesulfonic Acid: Synthesis, X-ray Crystal Analysis, and Multinuclear NMR Characterization of {(BuSn)12O14(OH)6}(4-CH3C6H4SO3)2

Author

EYCHENNE-BARON, Christophe, Ribot, François, Steunou, Nathalie, Sanchez, Clément, Fayon, Franck, Biesemans, Monique, Martins, José, Willem, Rudolph, Laboratoire de Chimie de la Matière Condensée de Paris (site Paris VI) (LCMCP (site Paris VI)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Collège de France (CdF (institution))-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Matériaux Hybrides et Nanomatériaux (LCMCP-MHN), Laboratoire de Chimie de la Matière Condensée de Paris (LCMCP), Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Novel Advanced Nano-Objects (LCMCP-NANO), Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Chimie de la Matière Condensée de Paris (LCMCP), Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Centre de recherches sur les matériaux à haute température (CRMHT), Centre National de la Recherche Scientifique (CNRS), High-Resolution NMR Centre, Vrije Universiteit Brussel (VUB), High Resolution NMR Centre, and Vrije Universiteit Brussel

Subjects
[CHIM.CRIS]Chemical Sciences/Cristallography, [CHIM.INOR]Chemical Sciences/Inorganic chemistry, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2000

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