1. Xanthomonas transcriptome inside cauliflower hydathodes reveals bacterial virulence strategies and physiological adaptation at early infection stages
- Author
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Noël, Laurent D., Luneau, Julien, Cerutti, Aude, Roux, Brice, Carrère, Sébastien, Jardinaud, Marie-Françoise, Gaillac, Antoine, Gris, Carine, Lauber, Emmanuelle, Berthomé, Richard, Arlat, Matthieu, Boulanger, Alice, Noël, Laurent, Laboratoire des Interactions Plantes Microbes Environnement (LIPME), Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), the COST action CA16107 EuroXanth., ANR-10-GENM-0013,XANTHOMIX,Etude comparative des génomes et des transcriptomes de Xanthomonas phytopathogènes(2010), ANR-10-JCJC-1703,XOPAQUE,Immunité vasculaire des plantes dépendante de XopAC(2010), ANR-18-CE20-0020,NEPHRON,Analyse génétique et moléculaire de l'immunité de l'hydathode et du système vasculaire(2018), ANR-19-CE20-0014,XBOX,L'étoffe d'un pathogène : comment Xanthomonas s'adapte à la vie in planta(2019), ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010), and ANR-11-IDEX-0002,UNITI,Université Fédérale de Toulouse(2011)
- Subjects
Xanthomonas ,Soil Science ,Virulence ,Brassica ,adaptation ,Plant Science ,Xanthomonas campestris ,Microbiology ,Transcriptome ,03 medical and health sciences ,Bacterial Proteins ,type III secretion ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Molecular Biology ,Pathogen ,Gene ,Plant Diseases ,030304 developmental biology ,0303 health sciences ,biology ,030306 microbiology ,hrpG ,Gene Expression Regulation, Bacterial ,Original Articles ,hrp gene cluster ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,biology.organism_classification ,Adaptation, Physiological ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Hydathode ,hydathode ,Regulon ,Original Article ,Agronomy and Crop Science ,type III effector - Abstract
Xanthomonas campestris pv. campestris (Xcc) is a seed‐transmitted vascular pathogen causing black rot disease on cultivated and wild Brassicaceae. Xcc enters the plant tissues preferentially via hydathodes, which are organs localized at leaf margins. To decipher both physiological and virulence strategies deployed by Xcc during early stages of infection, the transcriptomic profile of Xcc was analysed 3 days after entry into cauliflower hydathodes. Despite the absence of visible plant tissue alterations and despite a biotrophic lifestyle, 18% of Xcc genes were differentially expressed, including a striking repression of chemotaxis and motility functions. The Xcc full repertoire of virulence factors had not yet been activated but the expression of the HrpG regulon composed of 95 genes, including genes coding for the type III secretion machinery important for suppression of plant immunity, was induced. The expression of genes involved in metabolic adaptations such as catabolism of plant compounds, transport functions, sulphur and phosphate metabolism was upregulated while limited stress responses were observed 3 days postinfection. We confirmed experimentally that high‐affinity phosphate transport is needed for bacterial fitness inside hydathodes. This analysis provides information about the nutritional and stress status of bacteria during the early biotrophic infection stages and helps to decipher the adaptive strategy of Xcc to the hydathode environment., In planta transcriptomic analysis of Xanthomonas campestris inside cauliflower hydathodes reveals the adaptative processes at play during early infection.
- Published
- 2021