1. Electrochemical sensor for the detection of estradiol based on electropolymerized molecularly imprinted polythioaniline film with signal amplification using gold nanoparticles
- Author
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Cecilia Cristea, Nicole Jaffrezic-Renault, Anca Florea, Francis Vocanson, Robert Săndulescu, Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Université Cluj-Napoca, facultatea, Laboratoire Hubert Curien [Saint Etienne] (LHC), Institut d'Optique Graduate School (IOGS)-Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS), and 'This paper was published under the frame of European Social Found, Human Resources Development Operational Programme 2007-2013, project no. POSDRU/159/1.5/S/136893'.
- Subjects
17-BETA-ESTRADIOL ,02 engineering and technology ,Electrochemistry ,01 natural sciences ,lcsh:Chemistry ,[CHIM.ANAL]Chemical Sciences/Analytical chemistry ,Gold nanoparticles ,Detection limit ,Molecularly imprinted polymer ,p-Aminothiophenol ,Chromatography ,Estradiol ,Chemistry ,010401 analytical chemistry ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Electrochemical gas sensor ,Linear range ,lcsh:Industrial electrochemistry ,lcsh:QD1-999 ,Colloidal gold ,Linear sweep voltammetry ,POLYMERS ,0210 nano-technology ,Selectivity ,[CHIM.OTHE]Chemical Sciences/Other ,lcsh:TP250-261 - Abstract
A sensitive electrochemical molecularly imprinted sensor was developed for the detection of estradiol, by electropolymerization of p-aminothiophenol functionalized gold nanoparticles in the presence of estradiol as template molecule. The extraction of the template leads to the formation of cavities that are able to recognize and bind estradiol with high affinity. The performance of the developed sensor for the detection of estradiol was investigated by linear sweep voltammetry using a hexacyanoferrate/hexacyanoferrite solution as redox probe. The molecularly imprinted sensor exhibits a broad linear range, between 3.6 fM and 3.6 nM and a limit of quantification of 1.09 fM. Compared to the non-imprinted sensor, the imprinted sensor exhibits high affinity for the binding of estradiol. Moreover, selectivity studies, performed towards binding of testosterone, a hormone with similar chemical structure, proved high sensor selectivity. Furthermore, the molecularly imprinted sensor was applied for the analysis of spiked river samples with good recoveries. Keywords: Molecularly imprinted polymer, p-Aminothiophenol, Gold nanoparticles, Estradiol
- Published
- 2015