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Introduction: antioxidants play an important role in the antioxidant defense system. Obesity and the aging process increase oxidative stress. The disruption of the oxidant-antioxidant balance towards oxidant condition could be related to cognitive and physical disabilities. Objective: to evaluate the consumption of antioxidants, the oxidative stress status and their relationship with cognitive and functional alterations in aged with obesity. Method: a cross-sectional study was conducted with 94 subjects ≥ 60 years stratified by body mass index (BMI) (76.6% were women). The antioxidants intake as well as cognitive and functional status were evaluated by validated surveys. The oxidative stress markers were thiobarbituric acid reactive substances (TBARS) and lipid-hydroperoxides (LOOH). Results: the antioxidants consumption of the participants was below the recommended daily intakes (RDI) independently of the BMI group. Especially, a lower consumption of vitamins E and D, selenium and -carotenes was observed. The concentration of TBARS was higher (p < 0.05) in the obese (1.2 µM) versus the normal weight group (0.7 µM). The concentration of LOOH in the normal weight group (17 µM) did not show statistical difference versus obese group (15 µM). The presence of obesity in aged showed an association with walking difficulties. Multivariable analysis adjusted for several variables showed that the intake of selenium, copper and magnesium is associated with lower risk of cognitive and physical disabilities. Conclusions: aged with obesity showed a higher concentration of peripheral TBARS, walking difficulties and lower intake of antioxidants. The consumption of copper, selenium, and magnesium seems to protect against cognitive and physical disabilities in aged with obesity. Resumen Introducción: los antioxidantes son indispensables en el sistema de defensa antioxidante. La obesidad y el envejecimiento aumentan el estrés oxidativo. El desequilibrio del balance oxidante-antioxidante hacia una condición oxidante se relaciona con discapacidad cognitiva y física. Objetivos: evaluar la ingesta de antioxidantes, el estrés oxidativo y su relación con el estado cognitivo y funcional en ancianos con obesidad. Métodos: estudio transversal en 94 personas ≥ 60 años estratificadas por índice de masa corporal (IMC) (el 76,6% fueron mujeres). La ingesta de antioxidantes, el estado funcional y el estado cognitivo fueron evaluados con pruebas validadas. Los marcadores de estrés oxidativo fueron las sustancias reactivas al ácido tiobarbitúrico (TBARS) e hidroperóxidos lipídicos (LOOH). Resultados: la ingesta de antioxidantes de los participantes fue menor a la ingesta diaria recomendada (IDR), independientemente del grupo de IMC. La concentración de TBARS fue mayor (p < 0,05) en el grupo con obesidad (1,2 µM) en comparación con el grupo de peso normal (0,7 µM). La concentración de LOOH no fue estadísticamente diferente entre el grupo de peso normal (17 µM) y el grupo con obesidad (15 µM). La obesidad en ancianos se asoció a dificultad al caminar. El análisis multivariado ajustado por múltiples variables mostró que la ingesta de selenio, cobre y magnesio se asocia con menor riesgo de discapacidad cognitiva y física. Conclusiones: los ancianos con obesidad mostraron mayores concentraciones periféricas de TBARS, dificultad al caminar y una ingesta de antioxidantes deficiente. El consumo de cobre, selenio y magnesio parece proteger de discapacidades cognitivas y físicas a los ancianos.

Introduction: obesity affects more than a third of Mexican population. Oxidative stress participates actively in the etiology of this phenomenon. Glutathione peroxidase-1 (GPX-1) plays a protective role against oxidative stress. The SNP Pro200Leu (rs10504050) has been reported to affect the activity of the enzyme. Objective: to determine the frequency of rs10504050 polymorphism in women with obesity and normal weight control, asses the concentration of peripheral TBARS and evaluate the consumption of pro and antioxidants. Methods: 104 women with obesity and 70 healthy controls (CG) were included in the study. Anthropometric, biochemical, clinical and dietary features were evaluated. GPx-1 rs10504050 was determined by PCR/RFLP method. TBARS was assayed spectrophotometrically in plasma. The subjects were stratified and compared by obesity grades and by subgroups of prediabetes and diabetes condition. Statistical analysis included ANOVA of Kruskal Wallis, Xi squared and Pearson correlation. Results: for rs10504050 polymorphism there were differences (Xi² = 6; p = 0.01) between frequency (0.61) of obese carriers (Pro/Leu plus Leu/Leu) and CG carriers (0.42), and between (Xi² = 8; p = 0.004) morbid (IMC > 40) obesity (0.74) and CG carriers. The obese group (OB) showed a prevalence of 66% of prediabetes plus diabetes. There were no differences in frequencies of rs10504050 in OB with pre or diabetes versus CG, or versus obese participants without diabetes. TBARS concentration was greater in all the degrees of OB versus CG. Conclusion: GPx-1 Pro200Leu polymorphism was associated with obesity especially with morbid obesity, but not with obese participants with prediabetes or diabetes. Oxidative stress is present in all grades of obesity significantly. Introducción: la obesidad afecta a una tercera parte de la población mexicana. El estrés oxidativo (EO) participa activamente en la etiología del fenómeno. La glutatión peroxidasa-1 (GPx-1) juega un papel protector contra el EO. El SNP Pro200Leu (rs10504050) afecta a la actividad de la enzima. Objetivo: determinar la frecuencia del polimorfismo rs10504050 en mujeres con obesidad (OB) y normopeso (CG), determinar la concentración de TBARS en sangre periférica y evaluar el consumo de pro y antioxidantes. Métodos: en el estudio se incluyeron 104 mujeres con obesidad y 70 controles. El polimorfismo rs10504050 se determinó por el método PCR/RFLP. La concentración de TBARS se cuantificó mediante espectrofotometría en plasma sanguíneo. Las participantes se estratificaron y compararon por grados de obesidad y subgrupos de prediabetes y diabetes. Se emplearon las pruebas estadísticas ANOVA de Kruskal Wallis, Xi cuadrada y correlación de Pearson. Resultados: el polimorfismo rs10504050 mostró diferencias estadísticas (Xi² = 6; p = 0,01) entre la frecuencia del grupo OB (0,61) por arrastre (Pro/Leu+Leu/Leu) y el CG (0,42), así como (Xi² = 8; p = 0,004) entre personas con obesidad mórbida (0,74) comparadas con el CG. No hubo diferencia significativa entre las frecuencias del rs10504050 en OB con pre o diabetes, comparado con el CG, ni con personas con obesidad sin diabetes. Las concentraciones de TBARS fueron mayores en todos los grados de OB comparados con el CG. Conclusión: el polimorfismo rs10504050 se asoció con obesidad, especialmente mórbida, pero no se asoció con diabetes o prediabetes. El estrés oxidativo está presente de manera significativa en todos los grados de obesidad.

Introduction: obesity affects more than a third of Mexican population. Oxidative stress participates actively in the etiology of this phenomenon. Glutathione peroxidase-1 (GPX-1) plays a protective role against oxidative stress. The SNP Pro200Leu (rs10504050) has been reported to affect the activity of the enzyme. Objective: to determine the frequency of rs10504050 polymorphism in women with obesity and normal weight control, asses the concentration of peripheral TBARS and evaluate the consumption of pro and antioxidants. Methods: 104 women with obesity and 70 healthy controls (CG) were included in the study. Anthropometric, biochemical, clinical and dietary features were evaluated. GPx-1 rs10504050 was determined by PCR/RFLP method. TBARS was assayed spectrophotometrically in plasma. The subjects were stratified and compared by obesity grades and by subgroups of prediabetes and diabetes condition. Statistical analysis included ANOVA of Kruskal Wallis, Xi squared and Pearson correlation. Results: for rs10504050 polymorphism there were differences (Xi² = 6; p = 0.01) between frequency (0.61) of obese carriers (Pro/Leu plus Leu/Leu) and CG carriers (0.42), and between (Xi² = 8; p = 0.004) morbid (IMC > 40) obesity (0.74) and CG carriers. The obese group (OB) showed a prevalence of 66% of prediabetes plus diabetes. There were no differences in frequencies of rs10504050 in OB with pre or diabetes versus CG, or versus obese participants without diabetes. TBARS concentration was greater in all the degrees of OB versus CG. Conclusion: GPx-1 Pro200Leu polymorphism was associated with obesity especially with morbid obesity, but not with obese participants with prediabetes or diabetes. Oxidative stress is present in all grades of obesity significantly.