1. BAKLA PROTEİNİ: MİKRODALGA DESTEKLİ ÖZÜTLEME KOŞULLARININ OPTİMİZASYONU VE KARAKTERİSTİK ÖZELLİKLERİNİN PEYNİRALTI SUYU PROTEİNİ İLE KARŞILAŞTIRILMASI.
- Author
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KARAKUŞ, Mehmet Şükrü
- Subjects
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POLYACRYLAMIDE gel electrophoresis , *FOURIER transform infrared spectroscopy , *PLANT proteins , *WHEY proteins , *FAVA bean , *RESPONSE surfaces (Statistics) - Abstract
In this study, microwave-assisted extraction of faba bean protein (BP) concentrate was optimized through the response surface methodology. Microwave power (250-500 W) and processing time (10-60 min) were chosen as independent variables and the effect of these parameters on protein yield was investigated. The highest protein yield (47.74%) was achieved at 481.70 W microwave power and 50.68 min processing time. The characteristic properties of BP obtained under optimum conditions were compared with whey protein. The characteristic bonds of the proteins were confirmed by Fourier transform infrared spectroscopy (FTIR) and molecular weights were determined by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-page). The solubility values of BP and whey protein were found to be as 80.23% and 91.12%, respectively (P <0.05). BP's foaming properties, emulsifying behavior, and stability coefficient resulted in superior to whey protein. The results showed plant proteins will be considered as alternative protein sources in food systems. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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