1. Evaluation of cytotoxic activity of Schisandra chinensis lignans.
- Author
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Smejkal K, Slapetová T, Krmenčík P, Babula P, Dall'Acqua S, Innocenti G, Vančo J, Casarin E, Carrara M, Kalvarová K, Dvorská M, Slanina J, Kramářová E, Julínek O, and Urbanová M
- Subjects
- Cell Line, Cell Line, Tumor, Cell Proliferation drug effects, Chemical Fractionation, Chromatography, High Pressure Liquid, Cytotoxins chemistry, Cytotoxins isolation & purification, Humans, Lignans chemistry, Lignans isolation & purification, Nuclear Magnetic Resonance, Biomolecular, Plant Extracts chemistry, Nicotiana drug effects, Apoptosis drug effects, Cytotoxins toxicity, Lignans toxicity, Schisandra chemistry
- Abstract
Using exhaustive chromatographic separation we have isolated (-)-tigloyl-deangeloyl-gomisin F as a novel dibenzocyclooctadiene lignan from schisandra chinensis. With the help of HPLC, we further isolated (+)-schisandrin, (+)-deoxyschisandrin, (+)-γ-schisandrin, (-)-gomisin J, (+)-gomisin A, (-)-gomisin N, (-)-tigloyl-gomisin P, (-)-wuweizisu C, (-)-gomisin D, rubrisandrin A, (-)-gomisin G, (+)-gomisin K (3) and (-)-schisantherin C. A full NMR description of (-)-schisantherin C was carried out with the aim to confirm previous reports of its structure. Compounds isolated were identified on the basis of UV, IR, (1)H- and (13)C-NMR and MS. The cytotoxicity of lignans was tested for the BY-2 cell line alone and as a synergistic effect with the cytotoxic agent camptothecin. Lignans showed various toxicity and synergistic and antagonistic effects on camptothecin-induced cytotoxicity. Cytotoxicity against colon cancer cell line LoVo was also tested., (© Georg Thieme Verlag KG Stuttgart · New York.)
- Published
- 2010
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