1. Inhibitory Activity of Plant Stilbenoids against Nitric Oxide Production by Lipopolysaccharide-Activated Microglia.
- Author
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Nassra, Merian, Krisa, Stéphanie, Papastamoulis, Yorgos, Kapche, Gilbert Deccaux, Bisson, Jonathan, André, Caroline, Konsman, Jan-Pieter, Schmitter, Jean-Marie, Mérillon, Jean-Michel, and Waffo-Téguo, Pierre
- Subjects
ANALYSIS of variance ,ANIMAL experimentation ,BIOLOGICAL assay ,CELL culture ,MICE ,MOLECULAR structure ,NITRIC oxide ,POLYMERASE chain reaction ,STATISTICS ,STILBENE ,WESTERN immunoblotting ,DATA analysis ,REVERSE transcriptase polymerase chain reaction ,LIPOPOLYSACCHARIDES ,DESCRIPTIVE statistics - Abstract
Microglia-driven inflammatory processes are thought to play an important role in ageing and several neurological disorders. Since consumption of a diet rich in polyphenols has been associated with anti-inflammatory and neuroprotective effects, we studied the effects of twenty-five stilbenoids isolated from Milicia excelsa, Morus alba, Gnetum africanum, and Vitis vinifera. These compounds were tested at 5 and 10 µM on BV-2 microglial cells stimulated with bacterial lipopolysaccharide. Ten stilbenoids reduced lipopolysaccharide-induced nitric oxide production at 5 and/or 10 µM. Two tetramers, E-vitisin A and E-vitisin B, were the most effective molecules. Moreover, they attenuated the expression of the inducible NO synthase protein and gene. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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