1. Untersuchung des N-myc Status beim Neuroblastom aus Knochenmarkzellen
- Author
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Kabisch H, Finckenstein Fg, and Brack T
- Subjects
Soft Tissue Neoplasm ,Bone marrow infiltration ,Non malignant ,Biology ,medicine.disease ,medicine.anatomical_structure ,Neuroblastoma ,Pediatrics, Perinatology and Child Health ,Gene duplication ,Cancer research ,medicine ,Bone marrow ,Stage iv ,Gene - Abstract
The analysis of the N-myc gene in bone marrow specimens at the time of initial diagnosis and as well at the time of relapse from patients with Neuroblastoma stage IV and bone marrow infiltration could give some informations about the N-myc status of these patients. In stage IV neuroblastoma patients with bone marrow infiltration an estimation of the N-myc gene amplification should be attempted, if otherwise no information about the tumor content of the N-myc gene could be gathered. In our investigation we could demonstrate a Southern-blot-analysis of 27 bone marrow specimens with respect to the N-myc gene status which correlated qualitatively well to the N-myc amplification detected later on in the corresponding tumor tissue. In six cases the tumor infiltrated bone marrow showed a clear amplification of the N-myc gene. Because of the contamination by non malignant cells in bone marrow there was a quantitative difference in the calculated N-myc gene copies between the examined bone marrow specimens and corresponding tumor tissue.
- Published
- 1991
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