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The effect of a single, primary, artificial infection with Trypanosoma evansi was studied in nine Friesian Holstein calves. Body temperature, packed cell volume (PCV) and parasitaemia measurements were obtained daily from each of the infected calves for up to 90 days after parasites were injected, body weights were monitored weekly. T. evansi infection had a marked depressive effect on PCV profiles and the rate of body weight gain.

During an outbreak of trypanosomiasis in cattle, buffalo and horses on the island of Madura, a survey was conducted to determine the prevalence of Trypanosoma evansi in the region. Blood samples were collected from 130 cattle and 147 buffalo in 5 villages, whole blood was examined for trypanosomes by the microhaematocrit (MHCT) method and serum samples were subjected to an enzyme linked immuno absorbent assay to detect T. evansi antibodies. T. evansi was detected by MHCT in 50% of the buffalo examined and 13% of the cattle, while antibodies were detected in 47% of the buffalo and 30% of the cattle. The outbreak was brought under control following the administration of Nagano (Suramin, Bayer) to infected animals and animals considered to be at risk of infection.

Theileria parva piroplasm infection of mouse erythrocytes was detected, following intraperitoneal inoculation of mice with irradiated cultures of T. parva-infected bovine lymphoid cells. Infection could not be transmitted to cattle using Rhipicephalus appendiculatus ticks.