1. Development of microsatellite markers for the endangered orchid Calanthe izu-insularis (Orchidaceae)
- Author
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Azusa Hayano, Miho Murayama, Yoshihisa Suyama, Azusa Ito, Yu Matsuki, Naoyuki Nakahama, Yuji Isagi, and Shingo Kaneko
- Subjects
0106 biological sciences ,0301 basic medicine ,Orchidaceae ,Calanthe ,biology ,Endangered species ,Zoology ,Locus (genetics) ,General Medicine ,biology.organism_classification ,010603 evolutionary biology ,01 natural sciences ,03 medical and health sciences ,030104 developmental biology ,Genetic marker ,Genetic variation ,Genetics ,Polymorphic Microsatellite Marker ,Microsatellite ,Molecular Biology - Abstract
Microsatellite markers were developed for the endangered orchid Calanthe izu-insularis (Orchidaceae). This species is unique to the Izu Islands in Japan. Unfortunately, its population size has decreased because of excessive collection for horticultural purposes. In addition, although natural hybridization between C. izu-insularis and C. discolor var. discolor has been reported, morphological differences between C. izu-insularis and the hybridized individuals remain unclear. Using next-generation sequencing, 11 polymorphic microsatellite markers were developed. All developed markers could amplify C. aristulifera and nine markers could amplify C. d. var. discolor, two other orchid species that are also endangered in Japan. The number of alleles and expected heterozygosity at each locus were 1-6 (mean, 2.35) and 0.00-0.79 (mean, 0.30), respectively. These microsatellite markers will help conservation geneticists in their investigation of the proportion of pure C. izu-insularis individuals in the Izu Islands.
- Published
- 2018
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