1. Bioinformatic Analysis of LEA Genes in Stout Camphor Tree (Cinnamomum micranthum f. Kanehirae).
- Author
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CAN, Tevfik H. and KUŞAKSIZ, Tamer
- Subjects
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GENE targeting , *CINNAMOMUM , *MEDICINAL plants , *FUNCTIONAL analysis , *ABIOTIC stress - Abstract
LEA proteins have an important role in the response of plants to abiotic stresses. Cinnamomum micranthum f. kanehirae, a medicinal and aromatic plant belonging to the Lauraceae family. The genome sequence of the Kanehirae or Stout Camphor tree was recently completed. Although there are studies on its genome, there are no studies on LEA genes. 57 LEA genes (CmiLEA) were identified in the Stout Camphor genome. CmiLEA was divided into 8 distinct clusters based on phylogenetic analysis. When the subcellular localizations of CmiLEA were examined, they were found to be localized mostly in the cytoplasm. A total of 13 genes targeting only one miRNA were identified. In CmiLEA, a total of 23 genes were found to have only exon regions and no introns. In total, 35 conserved motifs were identified, while there was only one conserved motif in CmiLEA-42. Consistent with the 3D structure results, CmiLEA-21, CmiLEA-31, CmiLEA-44, CmiLEA-45, and CmiLEA57 from the LEA-2 subfamily showed over 90% accuracy. The present study was the first in-silico analysis of LEA genes in Cinnamomum micranthum f. Kanehirae. It is thought that it may form a base for advanced functional analysis in Cinnamomum in future. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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