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Aim: Individuals receiving hemodialysis treatment may observe glucose fluctuations due to decreased plasma glucose concentration and insulin level. The aim of this study was to evaluate pre-dialysis blood glucose measurements in acute hemodialysis patients to reduce complications that may occur during this process. Methods: The study design was an observational-retrospective one. A total of 200 files belonging to the last 3 months in three centers were scanned between August 1, 2022, and September 30, 2022. While the files were being scanned, the 30-question “Hypoglycemia in Hemodialysis” patient form was used, and the IBM Statistical package for the social sciences 26.0 statistical program was used to evaluate the data. Results: Hypoglycemia developed during hemodialysis in 4 of 104 patients whose blood glucose was checked before hemodialysis and in 2 of 96 patients whose blood glucose was not measured. It was determined that there was no significant difference (p>0.05) in terms of the rate of development of hypoglycemia between patients whose blood glucose levels were checked before hemodialysis and those who did not. Conclusion: The study’s results suggest that acute hemodialysis patients’ pre-dialysis hypoglycemia does not influence the hypoglycemia that develops during the session.