1. Investigation of Physiological Effects Induced by Dehydroepiandrosterone in Human Endothelial Cells and Ovarian Cancer Cell Line
- Author
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Gül İpek Gündoğan, Hüsniye Doğruman, Cenk Kig, and Meriç Karacan
- Subjects
medicine.medical_specialty ,endocrine system ,Necrosis ,media_common.quotation_subject ,Pharmaceutical Science ,Dehydroepiandrosterone ,chemistry.chemical_compound ,dehydroepiandrosterone ,Pharmacy and materia medica ,Internal medicine ,polycyclic compounds ,medicine ,skin and connective tissue diseases ,Receptor ,Ovulation ,media_common ,huvec ,business.industry ,Acridine orange ,medicine.disease ,In vitro ,RS1-441 ,Endocrinology ,ovarian cancer ,chemistry ,Molecular Medicine ,Original Article ,mdah-2774 ,medicine.symptom ,Ovarian cancer ,Ethidium bromide ,business ,hormones, hormone substitutes, and hormone antagonists - Abstract
OBJECTIVES: Dehydroepiandrosterone (DHEA) is an endogenous hormone that acts as a ligand for several cellular receptors. An age-dependent decline in circulating levels of DHEA is linked to changes in various physiological functions. In gynecological clinical practice, DHEA is commonly prescribed to induce ovulation. Some clinical studies report a positive association between high serum concentrations of DHEA and an increased risk of developing ovarian cancer. However, the in vitro physiological effects of DHEA on ovarian cancerous cells have not been explored thus far. In this study, we aimed to investigate the physiological effects of DHEA treatment (0-200 μM, 24-72 hours) on MDAH-2774 human ovarian cancer cell line and primary HuVeC human endothelial cells. MATERIALS AND METHODS: The physiological effects of DHEA treatment (0-200 μM, 24-72 hours) on MDAH-2774 human ovarian cancer cell line and primary HuVeC human endothelial cells were investigated with the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test, acridine orange/ethidium bromide staining, and scratch assay. RESULTS: DHEA treatment promoted proliferation of the MDAH-2774 cancer cell line in a dose-dependent manner (r=0.6906, p
- Published
- 2021