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Outflow tract development of the heart is complex. The presence, differential growth and interactions of the various tissues through space and time contribute to the final development of the tract. This paper presents a novel interpretation of observations of outflow tract development, in particular of the aortic and pulmonary semilunar valves in embryos from the Shaner Collection at the University of Alberta. Three-dimensional reconstructions assist in the visualization of the spatial relationships of the developing valve tissues. In some embryos the aortic intercalated valve swelling is displaced proximally, giving rise to a bicuspid aortic semilunar valve more distally. In addition, the developing valve tissue first appears external to the myocardial cuff. The pulmonary semilunar valve regions appear to be more normal. This paper thus proposes a novel mechanism for generating a bicuspid aortic valve and also supports the idea that there is some independence of the aortic and pulmonary regions from each other during development., (Copyright © 2017 The Author(s). Published by Elsevier GmbH.. All rights reserved.)

The development of cartilage in the aortic and pulmonary valves of chick and quail was studied using histological, histochemical and immunohistochemical techniques. In both species, the earliest evidence of chondrogenesis is the formation of smooth muscle alpha-actin-negative prechondrogenic (type II collagen-negative) cellular condensations in the tunica media of the proximal aorta and pulmonary trunk, in front of or slightly distal to the valvular commissures. Such condensations are present as early as stage 37 of Hamburger-Hamilton in the aortic and pulmonary valves of the chick. In quail embryos, they form somewhat later, namely, at stage 38 in the aortic valves and stage 39 in the pulmonary valves. In the chick, synthesis of type II collagen starts in the central core of the aortic cellular condensations at stage 38. In the pulmonary valves of chick and aortic and pulmonary valves of quail, production of type II collagen does not begin until stage 40. This production then gradually increases toward the periphery of the condensations, which remain devoid of perichondrium prior to hatching. After birth, the condensations become transformed into hyaline cartilaginous foci. In the aortic valves of some chickens and quails, more or less extensive deposits of hyaline cartilage or fibrocartilage form along the attachments of the leaflets to their supporting sinuses. They develop later than the commissural cartilages. The present findings, together with previous data from the literature, suggest that the aortic and pulmonary valve cartilages differentiate from neural crest-derived nonmuscular cells.