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Start Over Author "Lena Nyberg" Publisher future science ltd
2 results on '"Lena Nyberg"'

1. Applications of optical DNA mapping in microbiology

Author

Diana Bogas, Lena Nyberg, Rui Pacheco, Nuno F. Azevedo, Jason P. Beech, Margarita Gomila, Jorge Lalucat, Célia M. Manaia, Olga C. Nunes, Jonas O. Tegenfeldt, and Fredrik Westerlund

Subjects
DNA-based identification, imaging technique, lab-on-a chip, microbiological clinical diagnosis, optical DNA mapping, Biology (General), QH301-705.5
Abstract

Optical mapping (OM) has been used in microbiology for the past 20 years, initially as a technique to facilitate DNA sequence–based studies; however, with decreases in DNA sequencing costs and increases in sequence output from automated sequencing platforms, OM has grown into an important auxiliary tool for genome assembly and comparison. Currently, there are a number of new and exciting applications for OM in the field of microbiology, including investigation of disease outbreaks, identification of specific genes of clinical and/or epidemiological relevance, and the possibility of single-cell analysis when combined with cell-sorting approaches. In addition, designing lab-on-a-chip systems based on OM is now feasible and will allow the integrated and automated microbiological analysis of biological fluids. Here, we review the basic technology of OM, detail the current state of the art of the field, and look ahead to possible future developments in OM technology for microbiological applications.

Published
2017
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2. Applications of optical DNA mapping in microbiology

Author

Margarita Gomila, Jason P. Beech, Fredrik Westerlund, Lena Nyberg, Olga C. Nunes, Jorge Lalucat, Jonas O. Tegenfeldt, Rui Pacheco, Célia M. Manaia, Nuno F. Azevedo, Diana Bogas, Faculdade de Engenharia, Instituto de Ciências Biomédicas Abel Salazar, and Veritati - Repositório Institucional da Universidade Católica Portuguesa

Subjects
DNA, Bacterial, 0301 basic medicine, Bacteria, Optical Imaging, 030106 microbiology, High-Throughput Nucleotide Sequencing, Sequence assembly, Genomics, Sequence Analysis, DNA, Biology, General Biochemistry, Genetics and Molecular Biology, DNA sequencing, Microbiology, 03 medical and health sciences, 030104 developmental biology, Optical imaging, Lab-On-A-Chip Devices, Biological fluids, Imaging technique, Single-Cell Analysis, Genome, Bacterial, Automated sequencing, Biotechnology
Abstract

Optical mapping (OM) has been used in microbiology for the past 20 years, initially as a technique to facilitate DNA sequence-based studies; however, with decreases in DNA sequencing costs and increases in sequence output from automated sequencing platforms, OM has grown into an important auxiliary tool for genome assembly and comparison. Currently, there are a number of new and exciting applications for OM in the field of microbiology, including investigation of disease outbreaks, identification of specific genes of clinical and/or epidemiological relevance, and the possibility of single-cell analysis when combined with cell-sorting approaches. In addition, designing lab-on-a-chip systems based on OM is now feasible and will allow the integrated and automated microbiological analysis of biological fluids. Here, we review the basic technology of OM, detail the current state of the art of the field, and look ahead to possible future developments in OM technology for microbiological applications. © 2017 Thermo Fisher Scientific Inc.

Published
2017

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