1. Quantification of co-trimoxazole in serum and plasma using MS/MS.
- Author
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Dijkstra JA, Alsaad NS, Hateren Kv, Greijdanus B, Touw DJ, and Alffenaar JW
- Subjects
- Humans, Anti-Infective Agents blood, Plasma metabolism, Tandem Mass Spectrometry methods, Trimethoprim, Sulfamethoxazole Drug Combination blood
- Abstract
Background: Co-trimoxazole is frequently used in the prophylaxis and treatment of Pneumocystis carinii pneumonia. High plasma concentrations of sulfamethoxazole or trimethoprim are correlated with toxicity. There is, however, a large variation in PK observed which can lead to underexposure or toxicity., Results: We developed a novel LC-MS/MS method to analyze the components of co-trimoxazole, trimethoprim and sulfamethoxazole and its metabolite sulfamethoxazole-N-acetyl. This new method is expeditious due to its limited sample preprocessing and a relatively short run-time of only 3 min., Conclusion: This new method met the US FDA requirements on linearity, selectivity, precision, accuracy, matrix effects, recovery and stability and is suitable for routine analysis and future prospective studies.
- Published
- 2015
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