1. Analysis of 8q24.21 miRNA cluster expression and copy number variation in gastric cancer.
- Author
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Anauate AC, Leal MF, Wisnieski F, Santos LC, Gigek CO, Chen ES, Calcagno DQ, Assumpção PP, Demachki S, Arasaki CH, Artigiani R, Rasmussen LT, Payão SM, Burbano RR, and C Smith MA
- Subjects
- Adult, Cell Line, Tumor, DNA Copy Number Variations, Female, Gene Expression Profiling, Humans, Male, Middle Aged, Up-Regulation, Gene Expression Regulation, Neoplastic, MicroRNAs genetics, Stomach Neoplasms genetics
- Abstract
Aim: To analyze gene expression and copy number of five miRNAs ( miR-1204 , miR-1205 , miR-1206 , miR-1207 and miR-1208 ) localized in this chromosome region in gastric cancer (GC). Materials & methods: 65 paired neoplastic and non-neoplastic specimens collected from GC patients and 20 non-neoplastic gastric tissues from cancer-free individuals were included in this study. The expression levels of the five miRNAs were accessed by real time qPCR and were correlated. Results: MiR-1207-3p , miR-1205 , miR-1207-5p and miR-1208 were upregulated in approximately 50% of GC tumors in relation to those of adjacent non-neoplastic tissues. MiR-1205 expression was associated with gain of gene copies and was upregulated in adjacent non-neoplastic samples relative to external controls. Conclusion: The coexpression of the 8q24 miRNAs indicated the role of miR-1205 in the initiation of gastric cancer development.
- Published
- 2019
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