1. The ratio of thioredoxin/Keap1 protein level is a predictor of distant metastasis in colorectal cancer
- Author
-
Wen-Ko Tseng, Jim-Ray Chen, Chung-Ching Hua, Chung-Wei Fan, Hui-Ping Chein, Tsan-Yu Hsieh, Liang-Che Chang, and Cheng-Cheng Hwang
- Subjects
Male ,0301 basic medicine ,Oncology ,medicine.medical_specialty ,Colorectal cancer ,Clinical Biochemistry ,bcl-X Protein ,Bcl-xL ,Mitochondrial Proteins ,03 medical and health sciences ,Thioredoxins ,Western blot ,Internal medicine ,Drug Discovery ,Phosphoprotein Phosphatases ,medicine ,Humans ,Neoplasm Metastasis ,Aged ,Kelch-Like ECH-Associated Protein 1 ,medicine.diagnostic_test ,biology ,business.industry ,Biochemistry (medical) ,Distant metastasis ,Middle Aged ,medicine.disease ,KEAP1 ,030104 developmental biology ,Tumor progression ,biology.protein ,Female ,Thioredoxin ,Colorectal Neoplasms ,business ,Cysteine - Abstract
Aim: Thioredoxin can reduce the cysteine group of Keap1 which could induce proteasome degradation of Nrf2, PGAM5 and Bcl-xL. Nrf2 regulates redox balance and Bcl-xL is anti-apoptotic and both are important in tumor progression. Methods: The protein levels of Keap1, thioredoxin, PGAM5 and Bcl-xL in the normal and tumor tissues of 64 subjects with colorectal cancer (CRC) were determined by western blot. Results: The tumor had higher Keap1 but lower PGAM5s and Bcl-xL protein expression than the normal tissue. The ratio of thioredoxin/Keap1 protein level in the normal (OR: 0.12; 95% CI: 0.02–0.83) or tumor tissue (OR: 0.17; 95% CI: 0.03–0.89) was a negative predictor for distant metastasis in CRC. Conclusion: Keap1-mediated degradation of PGAM5 and Bcl-xL may be active in CRC. The ratio of thioredoxin/Keap1 protein level may be useful for suggesting distant metastasis in CRC.
- Published
- 2017