1. Copolymeric micelles as efficient inert nanocarrier for hypericin in the photodynamic inactivation of Candida species
- Author
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Gabriel Batista Cesar, Daniella Renata Faria, Franciele Av Rodrigues-Vendramini, Terezinha Ie Svidzinski, Patrícia de Souza Bonfim-Mendonça, Renato Sonchini Gonçalves, Erika Seki Kioshima, Glaucia Sayuri Arita, Isis Rg Capoci, Wilker Caetano, Pollyanna C.V. Conrado, Karina Mayumi Sakita, Noboru Hioka, and Neli Pieralisi
- Subjects
0301 basic medicine ,Microbiology (medical) ,Chemistry ,030106 microbiology ,Biofilm ,Microbiology ,Micelle ,In vitro ,Hypericin ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,Cell culture ,medicine ,Nanocarriers ,Fluconazole ,medicine.drug - Abstract
Aim: To evaluate the efficacy of photodynamic inactivation (PDI) mediated by hypericin encapsulated in P-123 copolymeric micelles (P123-Hyp) alone and in combination with fluconazole (FLU) against planktonic cells and biofilm formation of Candida species Materials & methods: PDI was performed using P123-Hyp and an LED device with irradiance of 3.0 mW/cm2 . Results: Most of isolates (70%) were completely inhibited with concentrations up to 2.0 μmol/l of HYP and light fluence of 16.2 J/cm2. FLU-resistant strains had synergic effect with P123-HYP-PDI and FLU. The biofilm formation was inhibited in all species, in additional the changes in Candida morphology observed by scanning electron microscopy. Conclusion: P123-Hyp-PDI is a promising option to treat fungal infections and medical devices to prevent biofilm formation and fungal spread.
- Published
- 2019
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