1. Antibody-functionalized gold nanoparticles as tumor-targeting radiosensitizers for proton therapy
- Author
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Sha Li, Sandra Bouchy, Carine Michiels, Bernard Gallez, Olivier Feron, Stéphane Lucas, Philippe Martinive, Anne-Catherine Heuskin, Riccardo Marega, Ornella Fichera, Sébastien Penninckx, UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - SSS/IREC/FATH - Pôle de Pharmacologie et thérapeutique, and UCL - SSS/LDRI-Louvain Drug Research Institute
- Subjects
Radiation-Sensitizing Agents ,Cetuximab ,Metal Nanoparticles ,Medicine (miscellaneous) ,02 engineering and technology ,Proton Therapy ,General Materials Science ,Microscopy ,0303 health sciences ,Tumor ,biology ,Chemistry ,Radiation-Sensitizing Agents/chemistry ,Metal Nanoparticles/chemistry ,021001 nanoscience & nanotechnology ,Gold/chemistry ,Cell killing ,Colloidal gold ,Antibody ,0210 nano-technology ,A431 cells ,radiosentizing effects ,medicine.drug ,EGFR ,Biomedical Engineering ,Bioengineering ,Development ,Conjugated system ,Electron ,Antibodies ,Cell Line ,Proton Therapy/methods ,03 medical and health sciences ,Microscopy, Electron, Transmission ,Cell Line, Tumor ,medicine ,Transmission ,Humans ,Clonogenic assay ,030304 developmental biology ,protontherapy ,Cetuximab/chemistry ,nanoparticle uptake ,gold nanoparticles ,Cancer cell ,biology.protein ,Biophysics ,Gold ,Antibodies/chemistry - Abstract
AIM: This study aimed at developing antibody-functionalized gold nanoparticles (AuNPs) to selectively target cancer cells and probing their potential radiosensitizing effects under proton irradiation.MATERIALS & METHODS: AuNPs were conjugated with cetuximab (Ctxb-AuNPs). Ctxb-AuNP uptake was evaluated by transmission electron microscopy and atomic absorption spectroscopy. Radioenhancing effect was assessed using conventional clonogenic assay.RESULTS & CONCLUSION: Ctxb-AuNPs specifically bound to and accumulated in EGFR-overexpressing A431 cells, compared with EGFR-negative MDA-MB-453 cells. Ctxb-AuNPs enhanced the effect of proton irradiation in A431 cells but not in MDA-MB-453 cells. These data indicate, for the first time, that combining enhanced uptake by specific targeting and radioenhancing effect, using conjugated AuNPs, is a promising strategy to increase cell killing by protontherapy.
- Published
- 2019