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Aim: We review relevant anatomy of the iliac crest, and describe an interventional technique to maximize harvesting of desired progenitor cells with ultrasound to guide safe trochar placement., Materials & Methods: We validated the technique on both sides of the pelvis in four human cadavers., Results: Using ultrasound guidance, 32 BMA needles were placed in a safe zone along various portions of the iliac crest., Conclusion: Ultrasound guidance can improve accuracy of bone marrow aspirations form the iliac crest. Mastery of this procedure will facilitate cell harvest and aid in patient safety when procuring mesenchymal stem cells from a bone marrow source.