1. The influence of anisotropic nano- to micro-topography on in vitro and in vivo osteogenesis
- Author
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Cedryck Vaquette, Eleanor Jones, Abhigyan Satyam, Andrew English, Saso Ivanovski, Dietmar W. Hutmacher, Bhawana Tripathi, Graham P. Riley, Niall Rooney, Graham L. W. Cross, Manus J.P. Biggs, Ayesha Azeem, Nandita Basu, Pramod Kumar, Alan O'Riordan, Abhay Pandit, Dimitrios I. Zeugolis, and Jan Henkel
- Subjects
Male ,Materials science ,Biomedical Engineering ,Medicine (miscellaneous) ,Bioengineering ,Nanotechnology ,Development ,Microscopy, Atomic Force ,Soft lithography ,Focal adhesion ,Polylactic Acid-Polyglycolic Acid Copolymer ,Tissue engineering ,Biomimetic Materials ,Osteogenesis ,In vivo ,Nano ,medicine ,Animals ,Humans ,General Materials Science ,Lactic Acid ,Anisotropy ,Cells, Cultured ,Sheep, Domestic ,Extracellular Matrix Proteins ,Osteoblasts ,Tissue Engineering ,Tissue Scaffolds ,Cell Differentiation ,Osteoblast ,In vitro ,Up-Regulation ,Nanomedicine ,medicine.anatomical_structure ,Bone Substitutes ,Biophysics ,Polyglycolic Acid - Abstract
Aim: Topographically modified substrates are increasingly used in tissue engineering to enhance biomimicry. The overarching hypothesis is that topographical cues will control cellular response at the cell–substrate interface. Materials & methods: The influence of anisotropically ordered poly(lactic-co-glycolic acid) substrates (constant groove width of ˜1860 nm; constant line width of ˜2220 nm; variable groove depth of ˜35, 306 and 2046 nm) on in vitro and in vivo osteogenesis were assessed. Results & discussion: We demonstrate that substrates with groove depths of approximately 306 and 2046 nm promote osteoblast alignment parallel to underlined topography in vitro. However, none of the topographies assessed promoted directional osteogenesis in vivo. Conclusion: 2D imprinting technologies are useful tools for in vitro cell phenotype maintenance.
- Published
- 2015