Currently, the prediction of rupture risk in abdominal aortic aneurysms (AAAs) solely relies on maximum diameter. However, wall mechanics and hemodynamics have shown to provide better risk indicators. Patient-specific fluid-structure interaction (FSI) simulations based on a non-invasive image modality are required to establish a patient-specific risk indicator. In this study, a robust framework to execute FSI simulations based on time-resolved three-dimensional ultrasound (3D+t US) data was obtained and employed on a data set of 30 AAA patients. Furthermore, the effect of including a pre-stress estimation (PSE) to obtain the stresses present in the measured geometry was evaluated. The established workflow uses the patient-specific 3D+t US-based segmentation and brachial blood pressure as input to generate meshes and boundary conditions for the FSI simulations. The 3D+t US-based FSI framework was successfully employed on an extensive set of AAA patient data. Omitting the pre-stress results in increased displacements, decreased wall stresses, and deviating time-averaged wall shear stress and oscillatory shear index patterns. These results underline the importance of incorporating pre-stress in FSI simulations. After validation, the presented framework provides an important tool for personalized modeling and longitudinal studies on AAA growth and rupture risk., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Fonken, Maas, Nievergeld, van Sambeek, van de Vosse and Lopata.)