1. Adaptation of Lactobacillus casei Zhang to Gentamycin Involves an Alkaline Shock Protein
- Author
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Chenxia Cao, Wenyi Zhang, Lai-Yu Kwok, Heping Zhang, Zhihong Sun, Lina Li, and Huiling Guo
- Subjects
0301 basic medicine ,Microbiology (medical) ,Lactobacillus casei ,030106 microbiology ,Mutant ,lcsh:QR1-502 ,Microbiology ,lcsh:Microbiology ,03 medical and health sciences ,gentamycin ,Lactobacillus ,Gene ,Original Research ,chemistry.chemical_classification ,Strain (chemistry) ,biology ,Wild type ,proteomic analysis ,biology.organism_classification ,asp23 ,Amino acid ,030104 developmental biology ,chemistry ,alkaline shock protein ,Proteome ,Lactobacillus casei Zhang - Abstract
Lactobacillus (L. casei) Zhang is a koumiss-originated probiotic strain, which was used as a model in a long-term antibiotics-driven evolution experiment to reveal bacterial evolutionary dynamics; and we isolated gentamycin-resistant L. casei Zhang descendents. To decipher the gentamycin resistance mechanism, here we cultivated the parental L. casei Zhang and its descendent cells in an antibiotics-containing environment to compare their global protein expression profiles using the iTRAQ-based proteomic approach. A total of 72 proteins were significantly up-regulated (>2.0-fold, P < 0.05), whilst 32 proteins were significantly down-regulated
- Published
- 2017
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