1. microRNAs profiling of small extracellular vesicles from midbrain tissue of Parkinson’s disease
- Author
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Zhengzhe Li, Dongdong Chen, Renjie Pan, Yanbiao Zhong, Tianyu Zhong, and Zhigang Jiao
- Subjects
Cellular and Molecular Neuroscience ,Molecular Biology - Abstract
Small extracellular vesicles (sEVs) are generated by all types of cells during physiological or pathological conditions. There is growing interest in tissue-derived small extracellular vesicles (tdsEVs) because they can be isolated from a single tissue source. Knowing the representation profile of microRNA (miRNA) in midbrain tissue–derived sEVs (bdsEVs) and their roles is imperative for understanding the pathological mechanism and improving the diagnosis and treatment of Parkinson’s disease (PD). bdsEVs from a rat model of PD and a sham group were separated and purified using ultracentrifugation, size-exclusion chromatography (SEC), and ultrafiltration. Then, miRNA profiling of bdsEVs in both groups was performed using next-generation sequencing (NGS). The expression levels of 180 miRNAs exhibited significant differences between the two groups, including 114 upregulated and 66 downregulated genes in bdsEVs of PD rats compared with the sham group (p more...
- Published
- 2023
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