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Antibacterial resistance is by far one of the greatest challenges to global health. Many pharmaceutical or material strategies have been explored to overcome this dilemma. Of these, silver nanoparticles (AgNPs) are known to have a non-specific antibacterial mechanism that renders it difficult to engender silver-resistant bacteria, enabling them to be more powerful antibacterial agents than conventional antibiotics. AgNPs have shown promising antibacterial effects in both Gram-positive and Gram-negative bacteria. The aim of this review is to summarize the green synthesis of AgNPs as antibacterial agents, while other AgNPs-related insights (e.g., antibacterial mechanisms, potential toxicity, and medical applications) are also reviewed.