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Your search keyword '"Vliet, Arnoud H. M."' showing total 13 results
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13 results on '"Vliet, Arnoud H. M."'

1. A multiplex PCR assay for the differentiation of Mycobacterium tuberculosis complex reveals high rates of mixed-lineage tuberculosis infections among patients in Ghana

Author

Owusu, Wellington, primary, van Vliet, Arnoud H. M., additional, Riddell, Natalie E., additional, Stewart, Graham, additional, Akwani, Winifred C., additional, Aryeetey, Sherihane, additional, Arthur, Rejoice Agyeiwaa, additional, Sylverken, Augustina Angelina, additional, and Hingley-Wilson, Suzanne M., additional

Published
2023
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5. Development of Loop-Mediated Isothermal Amplification Rapid Diagnostic Assays for the Detection of Klebsiella pneumoniae and Carbapenemase Genes in Clinical Samples

Author

Poirier, Aurore C., primary, Kuang, Dai, additional, Siedler, Bianca S., additional, Borah, Khushboo, additional, Mehat, Jai W., additional, Liu, Jialin, additional, Tai, Cui, additional, Wang, Xiaoli, additional, van Vliet, Arnoud H. M., additional, Ma, Wei, additional, Jenkins, David R., additional, Clark, John, additional, La Ragione, Roberto M., additional, Qu, Jieming, additional, and McFadden, Johnjoe, additional

Published
2022
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13. The Use of Comparative Genomic Analysis for the Development of Subspecies-Specific PCR Assays for Mycobacterium abscessus .

Author

Akwani WC, van Vliet AHM, Joel JO, Andres S, Diricks M, Maurer FP, Chambers MA, and Hingley-Wilson SM

Subjects
Anti-Bacterial Agents, Genomics, Humans, Multiplex Polymerase Chain Reaction, Mycobacterium genetics, Mycobacterium Infections, Nontuberculous diagnosis, Mycobacterium Infections, Nontuberculous microbiology, Mycobacterium abscessus genetics
Abstract

Mycobacterium abscessus complex (MABC) is an important pathogen of immunocompromised patients. Accurate and rapid determination of MABC at the subspecies level is vital for optimal antibiotic therapy. Here we have used comparative genomics to design MABC subspecies-specific PCR assays. Analysis of single nucleotide polymorphisms and core genome multilocus sequence typing showed clustering of genomes into three distinct clusters representing the MABC subspecies M. abscessus , M. bolletii and M. massiliense . Pangenome analysis of 318 MABC genomes from the three subspecies allowed for the identification of 15 MABC subspecies-specific genes. In silico testing of primer sets against 1,663 publicly available MABC genomes and 66 other closely related Mycobacterium genomes showed that all assays had >97% sensitivity and >98% specificity. Subsequent experimental validation of two subspecies-specific genes each showed the PCR assays worked well in individual and multiplex format with no false-positivity with 5 other mycobacteria of clinical importance. In conclusion, we have developed a rapid, accurate, multiplex PCR-assay for discriminating MABC subspecies that could improve their detection, diagnosis and inform correct treatment choice., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Akwani, van Vliet, Joel, Andres, Diricks, Maurer, Chambers and Hingley-Wilson.)

Published
2022
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