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Sugar beet productivity is highly constrained by the root-knot nematode (RKN) Meloidogyne incognita. Eight sugar beet genotypes were screened under greenhouse conditions for their susceptibility to M. incognita according to an adapted quantitative scheme for assignment Canto-Saenz’s host suitability (resistance) designations (AQSCS). Besides, the degree of susceptibility or tolerance of the examined genotypes was recorded by the modified host-parasite index (MHPI) scale based on yield performance. In addition, single nucleotide polymorphism (SNP) was also determined. Sugar beet genotypes have been classified into four categories for their susceptibility or tolerance according to the AQSCS scale. The first category, the moderately resistant (MR) group implies only one variety named SVH 2015, which did not support nematode reproduction (RF≤1), and had less root damage (GI≈2). Second, the tolerant group (T) involving Lilly and Halawa KWS supported fairly high nematode reproduction (RF>1) with relatively plant damage (GI≤2). Whereas the susceptible (S) category involved four varieties, FARIDA, Lammia KWS, Polat, and Capella, which supported nematode reproduction factor (RF>1) with high plant damage (GI>2). The fourth category refers to the highly susceptible (HYS) varieties such as Natura KWS that showed (RF≤1) and very high plant damage (GI>2). However, the MHPI scale showed that Lammia KWS variety was shifted from the (S) category to the (T) category. Results revealed significant differences among genotypes regarding disease severity, yield production, and quality traits. The SVH 2015 variety exhibited the lowest disease index values concerning population density with 800/250 cm3 soils, RF=2, root damage/gall index (GI=1.8), gall size (GS=2.3), gall area (GA=3.7), damage index (DI=3.4), susceptibility rate (SR=2.4), and MHP index (MHPI=2.5). However, Lammia KWS showed the highest disease index values regarding population density with 8890/250 cm3 soils, RF= 22.2, GI= 4.8, and SR= 14.1. Meanwhile, Natura KWS the highest GS, GA and MHPI with 7.1, 8 and 20.9, respectively. The lowest DI was achieved by Capella (DI= 6) followed by Lammia KWS (DI= 5.9). For yield production, and quality traits, SVH 2015 exhibited the lowest reductions of sugar yields/beet's root with 11.1%. While Natura KWS had the highest reduction with 79.3%, as well as it showed the highest reduction in quality traits; including sucrose, T.S.S, and purity with 65, 27.3, and 51.9%, respectively. The amino acid alignment and prediction of the DNA sequences revealed the presence of five SNPs among all sugar beet verities.

Salinity is one of the major plant abiotic stresses increasing over time worldwide. The most important biological role of nitric oxide (NO) in plants is related to their development and growth under abiotic conditions. The present experiment was conducted to study the effect of salt stress (0 and 100 mM) and NO (0 and 80 μM) on two different ecotypes of Brassica rapa (L.): PTWG-HL and PTWG-PK. The different growth attributes, biochemical and physiological parameters, and the mineral contents were examined. The results indicated increased hydrogen peroxide (H2O2), relative membrane permeability, malondialdehyde (MDA), and Na+ content and decreased plant biomass in both ecotypes (PTWG-PK and PTWG-HL) under salt stress. In contrast, NO treatment resulted in increased plant biomass, chlorophyll content, and total soluble proteins and decreased H2O2, relative membrane permeability, MDA, total phenolic content, catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX), and Na+. The combined effect of salt stress and NO application increased the chlorophyll a content, total phenolic content, and total soluble proteins, but decreased H2O2, relative membrane permeability, MDA, and Na+. The response of carotenoids, anthocyanins, and K+, Ca2+, and Cl− ions varied in both ecotypes under all treatment conditions. The PTWG-PK ecotype showed maximum overall growth response with the application of NO. Henceforth, it is proposed that the molecular mechanisms associated with NO-induced stress tolerance in plants may be exploited to attain sustainability in agriculture under changing climate scenarios.

Arsenic (As) contamination in the soil adversely affects crop productivity, grain quality, and human health. A pot experiment was performed to assess the sole and combined effects of Si and biochar on growth, physiological and antioxidant defense mechanisms, yield, and grain quality of maize. Soil treatments comprised of control (no treatment), As, Si, biochar, Si + biochar, As + Si, As + biochar, and As + Si + biochar placed in a completely randomized design with three replications. As toxicity significantly reduced chlorophyll a (5.18%), chlorophyll b (33.87%), chlorophyll a + b (11.67%), and primary metabolites [soluble protein (54.93%), amino acids (24.85%), total soluble sugars (39.77%), and phenolic contents (25.88%)], while increasing the activities of enzymatic antioxidants such as superoxide dismutase (SOD) by 43.51%, peroxidase (POD) by 47.93%, catalase (CAT) by 47.98%, and ascorbate peroxidase (APX) by 59.02%, as well as that of lipid peroxidation in the leaves of maize. In addition, As contamination reduced the grain yield and yield-related attributes relative to the respective controls. Among the soil applications, the interactive effect of Si and biochar improved maize grain yield (12.12%) by triggering activities of enzymatic antioxidants and proline contents and reducing the H2O2 and MDA contents. The combined application of Si and biochar enhanced the Si contents in shoots of the control and As-contaminated plants, while significantly reducing As concentration in shoots (69%) and grains (142%). In conclusion, the combined application of Si and biochar was found to be a fruitful soil amendment strategy to improve the yield of maize and reduce the toxic limit of As under As-contaminated soil. The results of this study may be useful for the cultivation of food crops under AS-contaminated soils, but before commercial recommendation, more trails are required under field conditions.

Optimizing nitrogen (N) fertilization without sacrificing grain yield is a major concern of rice production system because most of the applied N has been depleted from the soil and creating environmental consequences. Hence, limited information is available about nutrient management (NM) performance at a specific site under alternate wetting and drying (AWD) irrigation compared to conventional permanent flooding (PF). We aimed to inquire about the performance of NM practices compared to the farmer’s fertilizer practice (FFP) under PF and AWD on rhizospheric nitrifier and denitrifier abundance, rice yield, plant growth, and photosynthetic parameters. Two improved NM practices; nutrient management by pig manure (NMPM); 40% chemical N replaced by pig manure (organic N), and nutrient management by organic slow-release fertilizer (NMSR); 40% chemical N replaced by organic slow-release N were compared. The results showed an increased total grain yield (16.06%) during AWD compared to PF. Compared to conventional FFP, NMPM, and NMSR significantly increased the yields by 53.84 and 29.67%, respectively, during AWD. Meanwhile, PF prompted a yield increase of 45.07 and 28.75% for NMPM and NMSR, respectively, (p < 0.05) compared to FFP. Besides, a significant correlation was observed between grain yield and nitrogen content during AWD (R2 = 0.58, p < 0.01), but no significant correlation was observed during PF. The NMPM contributed to photosynthetic attributes and the relative chlorophyll content under both watering events. Moreover, relatively higher abundances of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) were observed during AWD, and the highest value was found after the late panicle stage. Our results suggest that the AWD–NMPM model is the best option to stimulate nitrifier and denitrifier gene abundance and promote rice production.

Heavy metal (HM) contamination is a serious concern across the globe, and in recent times, HMs’ intensity has significantly increased, posing a serious threat to crop growth and productivity. Heavy metals pose serious health issues in humans by entering the human food chains. Therefore, it is direly needed to reduce the effects of HMs on plants and humans by adapting appropriate practices. In this context, application of micronutrients can be an essential practice to mitigate the toxic effects of HMs. Zinc (Zn) is a crucial nutrient needed for plant growth, and Zn application reduced the HM-induced toxicity in plants. This review highlights Zn’s role in mitigating the HMs toxicity in plants. We have systematically described the potential mechanisms mediated by Zn to mitigate HMs in plants. Zinc application reduced the HMs uptake and translocation plants, which is considered an essential mechanism of HM stress tolerance. Zn application also improves membrane stability, plant water relationship, nutrient uptake, photosynthetic performance, osmolytes accumulation, anti-oxidant activities, and gene expression. In addition to this, the Zn application substantially improves photosynthesis by enhancing the synthesis of photosynthetic pigments, photosystem activities, enzymatic activities, and maintaining photosynthetic apparatus structure, ensuring better growth under HM stress. Therefore, Zn nutrition could improve the plant performance under HM stress by modulating the plant’s physiological and biochemical functioning, anti-oxidant activities, osmolytes accumulation, and gene expression.

Cotton is considered as the main crop in the agricultural sector of Pakistan. Water deficiency in this region in recent years has reduced the chances of high yields of cotton. Selection and creation of high-yielding varieties of cotton, even in water deficit conditions, is one of urgent tasks of today. For this purpose, 40 diverse genotypes of upland cotton were screened in normal and water deficit conditions in triplicate arrangement under split plot in a randomized complete block design. All the genotypes showed significant difference under both water regimes. Ten upland cotton accessions were screened out as water deficit tolerant (VH-144, IUB-212, MNH-886, VH-295, IR-3701, AA-802, NIAB-111, NS-121, FH-113, and FH-142) and five as water deficit sensitive (IR-3, CIM-443, FH-1000, MNH-147, and S-12) based on seed cotton yield and stress susceptibility index. These tolerant and sensitive genotypes were crossed in line × tester mating design. For further evaluation of genetic material, the seed of 50 F1 crosses and their 15 parents were field planted under normal and water deficit conditions during next cotton growing season. Traits related to yield under the study showed significant variations among the accessions and their half sibs. The results of the principal component analysis (PCA) exhibited that total variation exhibited by factors 1 and factor 2 were 55.55 and 41.95%, respectively. PCA transformed the variables into three factors, and only two factors (F1 and F2) had eigenvalue > 1. The degree of dominance revealed that all parameters were highly influenced by non-additive gene action under both water regimes. Furthermore, the line VH-295 and tester CIM-443 had better yield performance under water deficit stress. The cross-combinations, viz., VH-144 × S-12, NIAB-111 × IR-3, and VH-295 × MNH-147, were the best for yield contributing traits. These combinations may be helpful for germplasm enhancement on large scale under water scarcity. All the studied traits have non-additive types of gene action suggesting the usage of these genotypes in cotton hybrid development program against water deficit tolerance.

Photosynthetic rate (Pn) and photosynthetic nitrogen use efficiency (PNUE) are the two important factors affecting the photosynthesis and nutrient utilization of plant leaves. However, the effect of N fertilization combined with foliar application of Fe on the Pn and PNUE of the maize crops under different planting patterns (i.e., monocropping and intercropping) is elusive. Therefore, this experiment was conducted to determine the effect of N fertilization combined with foliar application of Fe on the photosynthetic characteristics, PNUE, and the associated enzymes of the maize crops under different planting patterns. The results of this study showed that under intercropping, maize treated with N fertilizer combined with foliar application of Fe had not only significantly (p < 0.05) improved physio-agronomic indices but also higher chlorophyll content, better photosynthetic characteristics, and related leaf traits. In addition, the same crops under such treatments had increased photosynthetic enzyme activity (i.e., rubisco activity) and nitrogen metabolism enzymes activities, such as nitrate reductase (NR activity), nitrite reductase (NiR activity), and glutamate synthase (GOGAT activity). Consequently, intercropping enhanced the PNUE and soluble sugar content of the maize crops, thus increasing its yield compared with monocropping. Thus, these findings suggest that intercropping under optimal N fertilizer application combined with Fe foliation can improve the chlorophyll content and photosynthetic characteristics of maize crops by regulating the associated enzymatic activities. Consequently, this results in enhanced PNUE, which eventually leads to better growth and higher yield in the intercropping system. Thus, practicing intercropping under optimal nutrient management (i.e., N and Fe) could be crucial for better growth and yield, and efficient nitrogen use efficiency of maize crops.

Phosphorus (P) constraint can be alleviated by increasing C inputs, which can help to improve crop production and P fertilizer use efficiency. However, the effects of different manures on soil microbial biomass P (MBP) and P fractions as well as C fractions in calcareous soils remain poorly understood. Soil MBP pool involves the P mineralization and immobilization processes, potentially changing P fractions and P availability. Therefore, the effects of different manures on soil microbial biomass (MBP, MBC) pool, P, and C fractions and crop P utilization were evaluated in greenhouse experiments with maize plantation. Treatments included no manure (control), poultry manure (PM), cow manure (CM), goat manure (GM), mixed manure (MM), and three inorganic P (Pi) rates; P0: 0 mg kg-1, P50: 50 mg kg-1, and P100: P100 mg kg-1 (P2O5). For plant growth comparison, crop physiological growth indices, shoot P contents and total P uptake were increased by PM and P100 as compared to other treatments. The PM with P100 significantly increased the plant growth by inducing P uptake of ∼18% compared with control. The results exhibited that Pi (P100) combined with manure (PM) significantly (p < 0.05) increased the soil physicochemical properties, that is, 683.76 mg kg-1 total P, 21.5 mg kg-1 Olsen P, 4.26 g kg-1 SOC, 2.41 g kg-1 POC, as well as microbial biomass C and P increased by 152.84 mg kg-1 and 36.83 mg kg-1, respectively. Consequently, we concluded that PM with Pi (P100) application builds up soil microbial biomass, which is more beneficial for promoting P utilization for maize.

Globally, climate change could hinder future food security that concurrently implies the importance of investigating drought stress and genotype screening under stressed environments. Hence, the current study was performed to screen 45 diverse maize inbred lines for 18 studied traits comprising phenological, physiological, morphological, and yield characters under optimum and water stress conditions for two successive growing seasons (2018 and 2019). The results showed that growing seasons and water regimes significantly influenced (p < 0.01) most of the studied traits, while inbred lines had a significant effect (p < 0.01) on all of the studied traits. The findings also showed a significant increase in all studied characters under normal conditions compared to drought conditions, except chlorophyll content, transpiration rate, and proline content which exhibited higher levels under water stress conditions. Furthermore, the results of the principal component analysis indicated a notable distinction between the performance of the 45 maize inbred lines under normal and drought conditions. In terms of grain yield, the drought tolerance index (DTI) showed that Nub60 (1.56), followed by Nub32 (1.46), Nub66 (1.45), and GZ603 (1.44) were the highest drought-tolerant inbred lines, whereas Nub46 (0.38) was the lowest drought-tolerant inbred line. These drought-tolerant inbred lines were able to maintain a relatively high grain yield under normal and stress conditions, whereas those drought-sensitive inbred lines showed a decline in grain yield when exposed to drought conditions. The hierarchical clustering analysis based on DTI classified the forty-five maize inbred lines and eighteen measured traits into three column- and row-clusters, as inbred lines in cluster-3 followed by those in cluster-2 exhibited greater drought tolerance in most of the studied traits. Utilizing the multi-trait stability index (MTSI) criterion in this study identified nine inbred lines, including GZ603, as stable genotypes in terms of the eighteen studied traits across four environments. The findings of the current investigation motivate plant breeders to explore the genetic potential of the current maize germplasm, especially in water-stressed environments.

This study was conducted to develop a protocol for in vitro shoot multiplication and callus induction of various mung bean varieties to obtain enhanced phytochemical content with the help of elicitors. For shoot multiplication, two types of explants (shoot tips and nodal tips) of three varieties of mung bean (Mung NCM-13, MgAT-7, and MgAT-4) were used. Both types of explants from in vitro and in vivo sources were cultured on the MS medium supplemented with different concentrations (0.25–3.0 mg/L, increment of 0.5 mg/L) and combinations of BAP and IBA as independent treatments. For callus induction, leaf explants (in vitro source) were cultured on MS medium supplemented with 2,4-D (1–3 mg/L) alone or in combination with BAP or NAA (0.5 and 1.0 mg/L). For the enhanced production of phenolics and glycosides, calli were cultured on MS media supplemented with zinc oxide (0.5 mg/L) and copper oxide nanoparticles (0.5 mg/L) as nano-elicitors. Results showed that in vitro explants responded better in terms of shoot length, number of shoots, and number of leaves per explant when compared to in vivo explants. Moreover, shoot tips were better than nodal explants to in vitro culturing parameters. All three varieties showed the optimized results in the MS medium supplemented with 1 mg/L BAP, while roots were produced only in cultures fortified with 1 mg/L IBA. The leaf explants of in vitro and soil-grown plantlets showed a maximum callogenic response of 90 and 80%, respectively, on MS medium supplemented with 2,4-D (3 mg/ml). Maximum phenolic content (101.4 μg of gallic acid equivalent/g) and glycoside content (34 mg of amygdalin equivalent/g of plant material) was observed in the calli cultured on MS medium supplemented with 3 mg/L of 2,4-D. Furthermore, the addition of zinc oxide (0.5 mg/L) and copper oxide (0.5 mg/L) nanoparticles to the callus culture medium significantly enhanced the phenolic content of Mung NCM-13 (26%), MgAT-7 (25.6%), and MgAT-4 (22.7%). Glycosidic content was also found to be increased in Mung NCM-13 (50%), MgAT-7 (37.5%), and MgAT-4 (25%) varieties when compared to the control. It is suggested that elicitation of in vitro cultures of mung beans with nanoparticles could be an effective strategy for the enhanced production of secondary metabolites.

Seaweeds are non-vascular, photosynthetic that inhabit the coastal regions commonly within rocky intertidal or submerged reef-like habitats and have been one of the richest and most promising sources of bioactive primary and secondary metabolites with antimicrobial properties. They selectively absorb elements like Na, K, Ca, Mg, I, and Br from the seawater and accumulate them in their thalli. Padina antillarum (Kützing) Piccone is a member of Phaeophycota and has remarkable phycochemistry as well as bioactivity. The phycochemical tests of the different extracts showed the presence of alkaloids, terpenoids, saponins, tannins, steroids, and phenols. The relative percentage of Oxirane, tetradecyl (C16H32O), and Cyclononasiloxane (C18H54O9Si9) are higher while Tetrasiloxane (C16H50O7Si8) is lowest in Gas Chromatography – Mass Spectrometry analysis. FRAP, %inhibition, the total antioxidant value of P. antillarum was higher in methanolic extract. Hexane, chloroform extracts showed no zone of inhibition against Bacillus subtilis, Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, and Staphylococcus epidermidis. The methanolic extract of P. antillarum exhibits a maximum zone of inhibition against S. epidermidis (18.66 ± 0.09). Antifungal activity of the P. antillarum in hexane extract exhibited no zone of inhibition against Aspergillus niger and Penicillium notatum while the chloroform extract yields maximum zone (37 ± 0.012, 21.66 ± 0.03). Diabetes mellitus is one of the most familiar chronic diseases associated with carbohydrate metabolism. It is also an indication of co-morbidities such as obesity, hypertension, and hyperlipidemia which are metabolic complications of both clinical and experimental diabetes. The treatment of P. antillarum methanol extract in mice reduced the body weight loss, low level of triglycerides, and elevated HDL cholesterol level as compared to diabetic mice.

Productivity of maize (Zea mays L.) and nitrogen use efficiency (NUE) as affected by nitrogen application levels and timing were studied. The experimental design was a three-replication randomized complete block design (RCBD). The first factor was nitrogen levels (122, 240, 288 and 336 kg N/ha) and the second factor was nitrogen timing (50% of N at sowing and 50% of N before the first irrigation; T1, 50% of N at sowing and 50% of N before the second irrigation; T2 and 50% of N before the first irrigation and 50% of N before the second irrigation; T3). Results indicated that plant height, ear length, kernel weight, number of grains/rows, number of grains/ear and grain yields all increased significantly as nitrogen levels increased and the level of 336 kg N/ha significantly exhibiting the highest values in both seasons. In terms of nitrogen application time, maize yield parameters such as plant height, ear length, kernel weight/ear, number of grains/rows, number of grains/ear and grain yield were significantly affected by nitrogen timing, with the highest values obtained at T3 while the lowest values obtained at T1 in both seasons. The interaction had a significant impact on plant height and grain yield/ha, with the tallest plants, the highest yields and its components observed at 336 kg N/ha, with 50% of N applied during the first irrigation and 50% of N applied during the second. Furthermore, under the study conditions, NUE decreased dramatically as nitrogen levels increased and increased significantly as nitrogen application time changed.

This study revealed the possible effects of various levels of silver nanoparticle (AgNP) application on plant diseases and soil microbial diversity. It investigated the comparison between the application of AgNPs and two commercial nanoproducts (Zn and FeNPs) on the rhizobacterial population and Botrytis fabae. Two experiments were conducted. The first studied the influence of 13 AgNP concentration on soil bacterial diversity besides two other commercial nanoparticles, ZnNPs (2,000 ppm) and FeNPs (2,500 ppm), used for comparison and application on onion seedlings. The second experiment was designed to determine the antifungal activity of previous AgNP concentrations (150, 200, 250, 300, 400, and 500 ppm) against B. fabae, tested using commercial fungicide as control. The results obtained from both experiments revealed the positive impact of AgNPs on the microbial community, representing a decrease in both the soil microbial biomass and the growth of brown spot disease, affecting microbial community composition, including bacteria, fungi, and biological varieties. In contrast, the two commercial products displayed lower effects compared to AgNPs. This result clearly showed that the AgNPs strongly inhibited the plant pathogen B. fabae growth and development, decreasing the number of bacteria (cfu/ml) and reducing the rhizosphere. Using AgNPs as an antimicrobial agent in the agricultural domain is recommended.

BackgroundPlant-parasitic nematodes are one of the major constraints to soybean production around the world. Plant-parasitic nematodes cause an estimated $78 billion in annual crop losses worldwide, with a 10–15% crop yield loss on average. Consequently, finding and applying sustainable methods to control diseases associated with soybean is currently in serious need.MethodsIn this study, we isolated, purified, characterized, and identified a novel cyanobacterial strainOscillatoriasp. (blue-green alga). Based on its microscopic examination and 16S rRNA gene sequence, the aqueous and methanolic extracts ofOscillatoriawere used to test their nematicidal activity againstMeloidogyne incognitahatchability of eggs after 72 h of exposure time and juvenile mortality percentagein vitroafter 24, 48, and 72 h of exposure time and reduction percentage of galls, eggmass, female number/root, and juveniles/250 soil. Also, the efficacy of the extract on improving the plant growth parameter and chlorophyll content under greenhouse conditions on soybean plant cv. Giza 111 was tested. Finally, the expression of PR-1, PR-2, PR-5, and PR15 (encoding enzymes) genes contributing to plant defense in the case ofM. incognitainvasion was studied and treated withOscillatoriaextract.ResultsThe aqueous and methanolic extracts ofOscillatoriasp. had nematicidal activity againstM. incognita. The percentage of mortality and egg hatching ofM. incognitawere significantly increased with the increase of time exposure toOscillatoriaextract 96.7, 97, and 98 larvae mortality % with S concentration after 24, 48, and 72 h of exposure time. The aqueous extract significantly increased the percentage of Root-Knot nematodes (RKN) of egg hatching, compared with Oxamyl and methanol extract at 96.7 and 97% after 72 h and 1 week, respectively. With the same concentration in the laboratory experiment. Furthermore, water extracts significantly reduced the number of galls in soybean root, egg masses, and female/root by 84.1, 87.5, and 92.2%, respectively, as well as the percentage of J2s/250 g soil by 93.7%. Root, shoot lengths, dry weight, number of pods/plant, and chlorophyll content of soybean treated withOscillatoriawater extract were significantly higher than the control increasing by 70.3, 94.1, 95.5, and 2.02%, respectively. The plant defense system's gene expression was tracked using four important pathogenesis-related genes, PR-1, PR-2, PR-5, and PR15, which encode enzymes involved in plant defense.ConclusionsOscillatoriaextract is a potential nematicide against root-knot nematode invasion in soybean.

Ten-year-old lemon (Citrus limon L. cv. Eureka) was used during the 2019 and 2020 seasons to investigate the effect of AgNPs at control, 5, 7.5, and 10 mg/L as a foliar application on vegetative growth, yield, and fruit quality. The selected trees were subjected to agricultural practices applied in the field during the study. The results indicated that the foliar application of AgNPs positively improved the shoot length, total chlorophyll, flower, and fruit set percentage, fruit yield, physical and chemical characteristics of fruits, and leaf mineral composition from macro and micronutrients compared to control in both seasons. The foliar application of AgNPs at 10 mg/L showed the highest mean values followed by 7.5 and 5 mg/L, respectively, for the previous characteristics. The treated leaves and fruit peels were hydrodistillated to extract the essential oils (EOs), and GC–MS analysis of leaf EOs. The analysis of leaves EOs showed the presence of neral, geranial, neryl acetate, and limonene as the main abundant bioactive compounds. While in peel the main compounds were neral, geranial, neryl acetate, D-limonene, geraniol acetate, linalool, and citronellal. Toxin effect of both EOs from leaves and peels were evaluated on the rice weevils (Sitophilus oryzae) and the results indicated a higher effect of lemon peel EOs than leaves based on mortality percentage and the values of LC50 and LC95 mg/L. Melia azedarach wood samples loaded with the produced lemon EOs were evaluated for their antifungal activity against the molecularly identified fungus, Fusarium solani (acc # OL410542). The reduction in mycelial growth was increased gradually with the applied treatments. The most potent activity was found in lemon leaf EOs, while peel EOs showed the lowest reduction values. The mycelial growth reduction percentages reached 72.96 and 52.59%, by 0.1% leaf and peel EOs, respectively, compared with control.

This current study was performed to determine the influences of plant spacing, Nitrogen (N) fertilization rate and their effect, on growth traits, yield, and yield components of cotton (Gossypium barbadense L.) cv. Giza 97 during the 2019 and 2020 seasons. A split plot experiment in three replicates was utilized whereas the cotton seeds were planted at 20, 30, and 40 cm, as main plots and nitrogen at 75, 100, and 125%, was in subplots. The results revealed that the planting spacing at 40 cm significantly (p ≤ 0.01) increased plant height, number of fruiting branches per plant, number of bolls per plant, boll weight (BW), lint percentage (L%), seed cotton yield (SCY), lint cotton yield (LCY), seed index and lint index by 165.68 cm, 20.92, 23.93, 3.75 g, 42.01%, 4.24 ton/ha, 5.16 ton/ha, 12.05, 7.86, respectively, as average in both seasons. The application of N fertilizer rate at 125% caused a maximum increase in growth and yield parameters i.e., plant height (169.08 cm), number of vegetative branches (2.67), number of fruiting branches per plant (20.82), number bolls per fruiting branch (1.39), number of bolls per plant (23.73), boll weight (4.1 g), lint percent (41.9%), seed index (11.8 g), and lint index (8.2), while the plants treated with 100% N rates exhibited highest seed cotton yield (4.3 ton/ha) and lint cotton yield (5.6 ton/ha), as average in both seasons. Combining plant spacing at 40 cm between plants with a 100% N fertilizer rate recorded the highest lint cotton yield (5.67 ton/ha), while the highest seed cotton yield (4.43 and 4.50 ton/ha) was obtained from 125% N fertilizer rate under planting spacing 20 and 40 cm, respectively. Conclusively, a wide density (40 cm) with 125% N is a promising option for improved biomass, cotton growth, yield, physiological traits, and fiber quality.

Rice (Oryza sativa L.) is one of the major cereal crops worldwide with wheat and maize. A total of two field experiments were performed to evaluate the response of some rice cultivars to various foliar zinc (Zn) concentrations based on different measurements, such as agronomic, yield, yield compounds, and grain technological parameters. The experimental layout was a split plot in three replicates; the five rice cultivars (Skaha 101, Giza178, Yasmeen, Fourate, and Amber 33) were distributed in the main plots while the four foliar applications of Zn (1,500, 2,000, 2,500 mg/L besides spray water) were occupied the sub-plots. The findings showed significant differences among the five rice cultivars regarding plant height, grain yield, straw yield, biological yield, harvest index, 1,000-grain weight, panicle length, protein percentage, and grain Zn content. There is a significant effect of Zn on all plant attributes. A significant interaction between rice cultivars and foliar application of Zn was observed, whereas fertilizing Giza 178 with foliar application of Zn at the rate of 2,500 mg/L achieved the highest mean values of grain yield and straw yield, biological yield, harvest index, 1,000-grain weight, panicle length, protein %, and Zn content followed by Sakha 101 with Zn application at the rate of 2,000 mg/L, respectively, in both seasons. The rice cultivars significantly differed in hulling (%), broken (%), hardness, grain length, shape, amylose (%), gel consistency, and gelatinization temperature. Unfortunately, the commercial Zn product used was genotoxic to pollen grains with a higher rate of Zn. Aberrations were observed such as stickiness, ultrastructural changes in the exterior and interior walls, partially or fully degenerated grains, and shrunken and unfilled grains. This study concluded that using Zn application at the rate of 2,000 mg/L to protect human and environmental health, the side effects and toxicity of the local commercial Zn product market should be investigated before making recommendations to farmers.