Search

Cultivated sunflower holds a very narrow genetic base and the efficient utilization of available genetic diversity is very important for a successful breeding program. In the present study, 109 sunflower genotypes were assessed for diversity paneling through a combined approach of morphological and molecular markers analysis. Morphological parameters including days to flower initiation, days to flower completion, plant height, stem curvature, number of leaves per plant, leaf area, head diameter, hundred seed weight, and seed yield per plant were studied. Simple sequence repeats (40 DNA markers) were deployed for diversity profiling. Data were analyzed by both univariate and multivariate statistics. SD and coefficient of variation confirm the presence of significant amounts of genetic variation for all the morphological parameters. Cluster Analysis and Principal Component Analysis further confirm the presence of distinct grouping patterns in the studied material. Cluster analysis of both morphological and molecular analysis revealed that restorer lines tend to group separately from A, B, and open-pollinated lines. Further grouping, at the sub-cluster level, revealed six distinct sub-clusters in each of the two major clusters. In total, 12 genotypes, 6 CMS lines (CMS-HAP-12, CMS-HAP-54, CMS-HAP-56, CMS-HAP-99, CMS-HAP-111, and CMS-HAP-112) and 6 restorer lines (RHP-38, RHP-41, RHP-53, RHP-68, RHP-69, and RHP-71) could be used as potential parents for hybrid development. As genotypes of similar genetic backgrounds tend to group closer, it is deduced that one genotype with the highest seed yield per plant could be used for further hybrid breeding programs in sunflowers.

Rice is a staple food for more than 50% of the global population and it is one of the most valuable cereal crops. To fulfill the dietary requirement of the ever-growing world population, an increase in per-unit production of rice is direly required. In Pakistan, it stands as the 2nd in consumption after wheat, which is a staple food. A huge gap is observed between yield potential and actual yield of the aromatic rice cultivars at a farmer-field level. The significant limitations responsible for this gap are shortage of irrigation water, inappropriate application of fertilizers, less plant population, deficiency of micronutrients, and improper and poor plant protection measures. A field study was planned to assess the yield response and quality attributes of aromatic rice to three levels of zinc (Zn) and nitrogen (N) under three irrigation regimes (8-, 12-, and 16-acre inches) in the Sheikhupura and Sargodha districts of Pakistan. Irrigation treatments significantly influenced the growth, yield, and quality attributes; however, maximum improvement was observed by the application of irrigation at 12-acre inches. Among the Zn treatments, application of Zn at 10 kg ha–1 was observed to be more responsive to improving the growth and quality parameters of aromatic rice crops. In the case of N treatments, application of N at 140 kg ha–1 produced the maximum total tillers, as well as productive tillers per hill, spikelets per panicle, leaf area index, leaf area duration, crop growth rate, total dry matter, harvest index, kernel length, kernel width, and 1,000-kernel weight. Application of N at 140 kg ha–1 not only improved the growth attributes but also increased the net assimilation rate, photosynthetically active radiation, and radiation use efficiency, with respect to total dry matter and kernel yield. The maximum percentage of normal kernels and minimum percentage of opaque, abortive, and chalky kernels were also recorded by application of N at 140 kg ha–1. The outcomes of current experiments depicted that application of irrigational water, zinc, and nitrogen at 12-acre inches, 10, and 140 kg ha–1, respectively, are responsible to achieve maximum resource utilization efficiency, along with increased yield and quality of rice.