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7 results on '"Flahaut, Christophe"'

2. The Algal Polysaccharide Ulvan Induces Resistance in Wheat Against Zymoseptoria tritici Without Major Alteration of Leaf Metabolome

Author

de Borba, Marlon C., primary, Velho, Aline C., additional, Maia-Grondard, Alessandra, additional, Baltenweck, Raymonde, additional, Magnin-Robert, Maryline, additional, Randoux, Béatrice, additional, Holvoet, Maxime, additional, Hilbert, Jean-Louis, additional, Flahaut, Christophe, additional, Reignault, Philippe, additional, Hugueney, Philippe, additional, Stadnik, Marciel J., additional, and Siah, Ali, additional

Published
2021
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7. Isolation and Characterization of Bacteria Colonizing Acartia tonsa Copepod Eggs and Displaying Antagonist Effects against Vibrio anguillarum, Vibrio alginolyticus and Other Pathogenic Strains

Author

Yanath Belguesmia, Benoit Cudennec, Djamel Drider, Mahammed Zidour, Thierry Grard, Sami Souissi, Christophe Flahaut, Mickaël Chevalier, UMR1281, Stress Abiotiques et Différenciation des Végétaux Cultivés, Institut National de la Recherche Agronomique (INRA), Stress Abiotiques et Différenciation des Végétaux Cultivés (SADV), Institut National de la Recherche Agronomique (INRA)-Université de Lille, Sciences et Technologies, Université du Littoral Côte d'Opale (ULCO), and Flahaut, Christophe

Subjects
0301 basic medicine, Microbiology (medical), Vibrio anguillarum, [SDV]Life Sciences [q-bio], ved/biology.organism_classification_rank.species, lcsh:QR1-502, medicine.disease_cause, copepod eggs, Microbiology, lcsh:Microbiology, Acartia tonsa, 03 medical and health sciences, Listeria monocytogenes, medicine, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, 14. Life underwater, Original Research, Bacillus pumilus, Vibrio alginolyticus, biology, ved/biology, MALDI-TOF-MS, antagonism, antibacterial compounds, amicoumacin, biology.organism_classification, Antimicrobial, Shrimp, 030104 developmental biology, Bacteria
Abstract

International audience; Copepods represent a major source of food for many aquatic species of commercial interest for aquaculture such as mysis shrimp and early stages of fishes. For the purpose of this study, the culturable mesophilic bacterial flora colonizing Acartia tonsa copepod eggs was isolated and identified. A total of 175 isolates were characterized based on their morphological and biochemical traits. The majority of these isolates (70%) were Gram-negative bacteria. Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) was used for rapid identification of bacterial isolates. Here, 58% of isolates were successfully identified at the genus level and among them, 54% were identified at the species level. These isolates belong to 12 different genera and 29 species. Five strains, identified as Bacillus pumilus, named 18 COPS, 35A COPS, 35R COPS, 38 COPS, and 40A COPS, showed strong antagonisms against several potential fish pathogens including Vibrio alginolyticus, V. anguillarum, Listeria monocytogenes, and Staphylococcus aureus. Furthermore, using a differential approach, we show that the antimicrobial activity of the 35R COPS strain is linked primarily to the production of antimicrobial compounds of the amicoumacin family, as demonstrated by the specific UV-absorbance and the MS/MS fragmentation patterns of these compounds.

Published
2017
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