7 results on '"Chunmao Zhang"'
Search Results
2. Anti-Influenza Effect and Mechanisms of Lentinan in an ICR Mouse Model
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Huan Cui, Cheng Zhang, Chunmao Zhang, Zhuming Cai, Ligong Chen, Zhaoliang Chen, Kui Zhao, Sina Qiao, Yingchun Wang, Lijia Meng, Shishan Dong, Juxiang Liu, and Zhendong Guo
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Microbiology (medical) ,Mice, Inbred ICR ,Immunology ,Anti-Inflammatory Agents ,Microbiology ,Disease Models, Animal ,Mice ,Lentinan ,Infectious Diseases ,Orthomyxoviridae Infections ,Neoplasms ,Influenza, Human ,Animals ,Cytokines ,Humans - Abstract
Influenza virus is a serious threat to global human health and public health security. There is an urgent need to develop new anti-influenza drugs. Lentinan (LNT) has attracted increasing attention in recent years. As potential protective agent, LNT has been shown to have anti-tumor, anti-inflammatory, and antiviral properties. However, there has been no further research into the anti-influenza action of lentinan in vivo, and the mechanism is still not fully understood. In this study, the anti-influenza effect and mechanism of Lentinan were studied in the Institute of Cancer Research (ICR) mouse model. The results showed that Lentinan had a high degree of protection in mice against infection with influenza A virus, delayed the emergence of clinical manifestations, improved the survival rate of mice, significantly prolonged the middle survival days, attenuated the weight loss, and reduced the lung coefficient of mice. It alleviated the pathological damage of mice infected with the influenza virus and improved blood indices. Lentinan treatment considerably inhibited inflammatory cytokine (TNF-α, IL-1β, IL-4, IL-5, IL-6) levels in the serum and lung and improved IFN-γ cytokine levels, which reduced cytokine storms caused by influenza virus infection. The underlying mechanisms of action involved Lentinan inhibiting the inflammatory response by regulating the TLR4/MyD88 signaling pathway. This study provides a foundation for the clinical application of Lentinan, and provides new insight into the development of novel immunomodulators.
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- 2022
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3. Aerosol Transmission of the Pandemic SARS-CoV-2 and Influenza A Virus Was Blocked by Negative Ions
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Cheng Zhang, Huan Cui, Chunmao Zhang, Zhaoliang Chen, Xinyun Jiang, Jun Liu, Zhonghai Wan, Jiping Li, Juxiang Liu, Yuwei Gao, Ningyi Jin, and Zhendong Guo
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Aerosols ,Ions ,Microbiology (medical) ,SARS-CoV-2 ,Guinea Pigs ,Immunology ,COVID-19 ,virus diseases ,Microbiology ,Mice ,Infectious Diseases ,Influenza A virus ,Cricetinae ,Animals ,Pandemics - Abstract
The pandemic of respiratory diseases, such as coronavirus disease 2019 (COVID-19) and influenza, has imposed significant public health and economic burdens on the world. Wearing masks is an effective way to cut off the spread of the respiratory virus. However, due to cultural differences and uncomfortable wearing experiences, not everyone is willing to wear masks; there is an urgent need to find alternatives to masks. In this study, we tested the disinfection effect of a portable ionizer on pandemic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (strain V34) and influenza A virus (strain CA04). Negative ions significantly reduced the concentration of particulate matter in the air above and effectively disinfected viruses stuck to the solid plate at the level of both nucleic acid and virus titer. The disinfection efficiency was >99.8% after 1-h exposure. Moreover, negative ions effectively disinfected aerosolized viruses; the disinfection efficiency was more than 87.77% after purification for 10 min. Furthermore, negative ions had a significant protective effect on susceptible animals exposed to viral aerosols. When the negative ionizer was switched from off to on, the inhalation 50% infective dose (ID50) for golden hamsters challenged with SARS-CoV-2 rose from 9.878 median tissue culture infective dose (TCID50) [95% confidence interval (CI), 6.727–14.013 TCID50] to 43.891 TCID50 (95% CI, 29.31–76.983 TCID50), and the inhalation ID50 for guinea pigs challenged with influenza A virus rose from 6.696 TCID50 (95% CI, 3.251–9.601 TCID50) to 28.284 TCID50 (95% CI, 19.705–40.599 TCID50). In the experiment of transmission between susceptible animals, negative ions 100% inhibited the aerosol transmission of SARS-CoV-2 and influenza A virus. Finally, we tested the safety of negative ion exposure. Balb/c mice exposed to negative ions for 4 weeks showed no abnormalities in body weight, blood routine analysis, and lung pathology. Our study demonstrates that air ions can be used as a safe and effective means of blocking respiratory virus transmission and contribute to pandemic prevention and control.
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- 2022
4. The SARS-CoV-2 B.1.351 Variant Can Transmit in Rats But Not in Mice
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Cheng Zhang, Huan Cui, Entao Li, Zhendong Guo, Tiecheng Wang, Fang Yan, Lina Liu, Yuanguo Li, Di Chen, Keyin Meng, Nan Li, Chengfeng Qin, Juxiang Liu, Yuwei Gao, and Chunmao Zhang
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Mice, Inbred C57BL ,Mice ,Mice, Inbred BALB C ,SARS-CoV-2 ,Cricetinae ,Immunology ,Animals ,COVID-19 ,Humans ,Immunology and Allergy ,Rats - Abstract
Previous studies have shown that B.1.351 and other variants have extended the host range of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to mice. Sustained transmission is a prerequisite for viral maintenance in a population. However, no evidence of natural transmission of SARS-CoV-2 in wild mice has been documented to date. Here, we evaluated the replication and contact transmission of the B.1.351 variant in mice and rats. The B.1.351 variant could infect and replicate efficiently in the airways of mice and rats. Furthermore, the B.1.351 variant could not be transmitted in BALB/c or C57BL/6 mice but could be transmitted with moderate efficiency in rats by direct contact. Additionally, the B.1.351 variant did not transmit from inoculated Syrian hamsters to BALB/c mice. Moreover, the mouse-adapted SARS-CoV-2 strain C57MA14 did not transmit in mice. In summary, the risk of B.1.351 variant transmission in mice is extremely low, but the transmission risk in rats should not be neglected. We should pay more attention to the potential natural transmission of SARS-CoV-2 variants in rats and their possible spillback to humans.
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- 2022
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5. Impact of Prior Infection on Severe Acute Respiratory Syndrome Coronavirus 2 Transmission in Syrian Hamsters
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Cheng Zhang, Zhendong Guo, Nan Li, Huan Cui, Keyin Meng, Lina Liu, Li Zhao, Shanshan Zhang, Chengfeng Qin, Juxiang Liu, Yuwei Gao, and Chunmao Zhang
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Microbiology (medical) ,Transmission (medicine) ,business.industry ,viruses ,animal diseases ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,virus diseases ,medicine.disease_cause ,Microbiology ,Airborne transmission ,Virology ,QR1-502 ,reinfection ,Herd immunity ,Immunity ,Infectious disease (medical specialty) ,Pandemic ,medicine ,prior infection ,airborne transmission ,contact transmission ,business ,Original Research ,severe acute respiratory syndrome coronavirus 2 ,Coronavirus - Abstract
Prior infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) provides protective immunity against reinfection. However, whether prior infection blocks SARS-CoV-2 transmission is not yet clear. Here, we evaluated the impact of prior infection on SARS-CoV-2 transmission in Syrian hamsters. Our results showed that prior infection significantly reduced SARS-CoV-2 replication in Syrian hamsters, but sterilizing immunity was not achieved. Prior infection blocked the airborne transmission of SARS-CoV-2 from previously infected Syrian hamsters to naïve Syrian hamsters and previously infected Syrian hamsters. Moreover, prior infection substantially reduced the efficiency of direct contact transmission between previously infected Syrian hamsters. However, prior infection had limited impact on SARS-CoV-2 transmission from previously infected Syrian hamsters to naïve Syrian hamsters via direct contact in the early course of infection. Human reinfection and SARS-CoV-2 transmission between a previously infected population and a healthy population would be likely, and a higher vaccination coverage rate was needed to reach herd immunity. Our work will aid the implementation of appropriate public health and social measures to control coronavirus infectious disease 2019 (COVID-19) pandemic.
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- 2021
6. Risk of Environmental Exposure to H7N9 Influenza Virus via Airborne and Surface Routes in a Live Poultry Market in Hebei, China
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Cheng Zhang, Kangkang Guo, Huan Cui, Ligong Chen, Chunmao Zhang, Xuejing Wang, Jiaming Li, Yingying Fu, Zhongyi Wang, Zhendong Guo, Juxiang Liu, and Shishan Dong
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Microbiology (medical) ,H7N9 virus ,China ,Coronavirus disease 2019 (COVID-19) ,viruses ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Immunology ,Virulence ,Biology ,Influenza A Virus, H7N9 Subtype ,mammalian adaption ,Microbiology ,Poultry ,Virus ,Mice ,Cellular and Infection Microbiology ,human exposure risk ,Influenza, Human ,pathogenicity ,Animals ,Humans ,Original Research ,Transmission (medicine) ,Outbreak ,Environmental Exposure ,Environmental exposure ,Pathogenicity ,Virology ,QR1-502 ,Infectious Diseases ,Influenza in Birds ,environmental and airborne transmissibility - Abstract
Environmental transmission of viruses to humans has become an early warning for potential epidemic outbreaks, such as SARS-CoV-2 and influenza virus outbreaks. Recently, an H7N9 virus, A/environment/Hebei/621/2019 (H7N9), was isolated by environmental swabs from a live poultry market in Hebei, China. We found that this isolate could be transmitted by direct contact and aerosol in mammals. More importantly, after 5 passages in mice, the virus acquired two adaptive mutations, PB1-H115Q and B2-E627K, exhibiting increased virulence and aerosol transmissibility. These results suggest that this H7N9 virus might potentially be transmitted between humans through environmental or airborne routes.
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- 2021
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7. A Novel Reassortant Avian H7N6 Influenza Virus Is Transmissible in Guinea Pigs via Respiratory Droplets
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Zongzheng Zhao, Lina Liu, Zhendong Guo, Chunmao Zhang, Zhongyi Wang, Guoyuan Wen, Wenting Zhang, Yu Shang, Tengfei Zhang, Zuwu Jiao, Ligong Chen, Cheng Zhang, Huan Cui, Meilin Jin, Chengyu Wang, Qingping Luo, and Huabin Shao
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Microbiology (medical) ,receptor binding ,Reassortment ,lcsh:QR1-502 ,Virulence ,Hemagglutinin (influenza) ,medicine.disease_cause ,Microbiology ,lcsh:Microbiology ,Virus ,03 medical and health sciences ,medicine ,Influenza A virus ,pathogenicity ,transmissibility ,avian H7N6 influenza A virus ,Gene ,Original Research ,030304 developmental biology ,0303 health sciences ,biology ,030306 microbiology ,Virology ,Influenza A virus subtype H5N1 ,biology.protein ,reassortment ,Neuraminidase - Abstract
Since 2013, H7N9 and H5N6 avian influenza viruses (AIVs) have caused sporadic human infections and deaths and continued to circulate in the poultry industry. Since 2014, H7N6 viruses which might be reassortants of H7N9 and H5N6 viruses, have been isolated in China. However, the biological properties of H7N6 viruses are unknown. Here, we characterize the receptor binding preference, pathogenicity and transmissibility of a H7N6 virus A/chicken/Hubei/00095/2017(H7N6) (abbreviated HB95), and a closely related H7N9 virus, A/chicken/Hubei/00093/2017(H7N9) (abbreviated HB93), which were isolated from poultry in Hubei Province, China, in 2017. Phylogenetic analyses demonstrated that the hemagglutinin (HA) gene of HB95 is closely related to those of HB93 and human-origin H7N9 viruses, and that the neuraminidase (NA) gene of HB95 shared the highest nucleotide similarity with those of H5N6 viruses. HB95 and HB93 had binding affinity for human-like α2, 6-linked sialic acid receptors and were virulent in mice without prior adaptation. In addition, in guinea pig model, HB93 was transmissible by direct contact, but HB95 was transmissible via respiratory droplets. These results revealed the potential threat to public health posed by H7N6 influenza viruses and emphasized the need for continued surveillance of the circulation of this subtype in poultry.
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- 2019
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