1. Unearthing the Plant Growth-Promoting Traits of Bacillus megaterium RmBm31, an Endophytic Bacterium Isolated From Root Nodules of Retama monosperma
- Author
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Malika Affaf Dahmani, Antoine Desrut, Bouziane Moumen, Julien Verdon, Lamia Mermouri, Mourad Kacem, Pierre Coutos-Thévenot, Meriem Kaid-Harche, Thierry Bergès, Cécile Vriet, Signalisation et Transports Ioniques Membranaires (STIM), Université de Poitiers-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Ecologie et biologie des interactions (EBI), Université de Poitiers-Centre National de la Recherche Scientifique (CNRS), Sucres & Echanges Végétaux-Environnement (SEVE), Université de Poitiers-Centre National de la Recherche Scientifique (CNRS)-Université de Poitiers-Centre National de la Recherche Scientifique (CNRS), Ecologie, Evolution, Symbiose (EES), Microbiologie de l'Eau (MDE), and Université de Poitiers-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0106 biological sciences ,0301 basic medicine ,Root nodule ,plant growth-promoting rhizobacteria ,Retama monosperma ,Arabidopsis thaliana ,Perennial plant ,[SDV]Life Sciences [q-bio] ,Plant Science ,lcsh:Plant culture ,Rhizobacteria ,01 natural sciences ,Endophyte ,03 medical and health sciences ,Botany ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,lcsh:SB1-1110 ,[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM] ,ComputingMilieux_MISCELLANEOUS ,Bacillus megaterium ,2. Zero hunger ,biology ,fungi ,food and beverages ,15. Life on land ,biology.organism_classification ,030104 developmental biology ,root nodules ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,endophyte ,Bacteria ,010606 plant biology & botany - Abstract
Plants live in association with complex populations of microorganisms, including Plant Growth-Promoting Rhizobacteria (PGPR) that confer to plants an improved growth and enhanced stress tolerance. This large and diverse group includes endophytic bacteria that are able to colonize the internal tissues of plants. In the present study, we have isolated a nonrhizobial species from surface sterilized root nodules of Retama monosperma, a perennial leguminous species growing in poor and high salinity soils. Sequencing of its genome reveals this endophytic bacterium is a Bacillus megaterium strain (RmBm31) that possesses a wide range of genomic features linked to plant growth promotion. Furthermore, we show that RmBm31 is able to increase the biomass and positively modify the root architecture of seedlings of the model plant species Arabidopsis thaliana both in physical contact with its roots and via the production of volatile organic compounds. Lastly, we investigated the molecular mechanisms implicated in RmBm31 plant beneficial effects by carrying out a transcriptional analysis on a comprehensive set of phytohormone-responsive marker genes. Altogether, our results demonstrate that RmBm31 displays plant growth-promoting traits of potential interest for agricultural applications.
- Published
- 2020