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Depression is an undetectable mental disease. Most of the patients with depressive symptoms do not know that they are suffering from depression. Since the novel Coronavirus pandemic 2019, the number of patients with depression has increased rapidly. There are two kinds of traditional depression diagnosis. One is that professional psychiatrists make diagnosis results for patients, but it is not conducive to large-scale depression detection. Another is to use electroencephalography (EEG) to record neuronal activity. Then, the features of the EEG are extracted using manual or traditional machine learning methods to diagnose the state and type of depression. Although this method achieves good results, it does not fully utilize the multi-channel information of EEG. Aiming at this problem, an EEG diagnosis method for depression based on multi-channel data fusion cropping enhancement and convolutional neural network is proposed. First, the multi-channel EEG data are transformed into 2D images after multi-channel fusion (MCF) and multi-scale clipping (MSC) augmentation. Second, it is trained by a multi-channel convolutional neural network (MCNN). Finally, the trained model is loaded into the detection device to classify the input EEG signals. The experimental results show that the combination of MCF and MSC can make full use of the information contained in the single sensor records, and significantly improve the classification accuracy and clustering effect of depression diagnosis. The method has the advantages of low complexity and good robustness in signal processing and feature extraction, which is beneficial to the wide application of detection systems.

Reasonable exercise is beneficial to human health. However, it is difficult for ordinary athletes to judge whether they are already in a state of fatigue that is not suitable for exercise. In this case, it is easy to cause physical damage or even life-threatening. Therefore, to health sports, protecting the human body in sports not be injured by unreasonable sports, this study proposes an exercise fatigue diagnosis method based on short-time Fourier transform (STFT) and convolutional neural network (CNN). The method analyzes and diagnoses the real-time electrocardiogram, and obtains whether the current exerciser has exercise fatigue according to the electrocardiogram. The algorithm first performs short-time Fourier transform on the electrocardiogram (ECG) signal to obtain the time spectrum of the signal, which is divided into training set and validation set. The training set is then fed into the convolutional neural network for learning, and the network parameters are adjusted. Finally, the trained convolutional neural network model is applied to the test set, and the recognition result of fatigue level is output. The validity and feasibility of the method are verified by the ECG experiment of exercise fatigue degree. The experimental recognition accuracy rate can reach 97.70%, which proves that the constructed sports fatigue diagnosis model has high diagnostic accuracy and is feasible for practical application.

Pseudonocardia species are emerging as important microorganisms of global concern with unique and increasingly significant ecological roles and represent a prominent source of bioactive natural products, but genetic engineering of these organisms for biotechnological applications is greatly hindered due to the limitation of efficient genetic manipulation tools. In this regard, we report here the establishment of an efficient genetic manipulation system for a newly isolated strain, Pseudonocardia alni Shahu, based on plasmid conjugal transfer from Escherichia coli to Pseudonocardia. Conjugants were yielded upon determining the optimal ratio between the donor and recipient cells, and designed genome modifications were efficiently accomplished, including exogenous gene integration based on an integrative plasmid and chromosomal stretch removal by homologous recombination using a suicidal non-replicating vector. Collectively, this work has made the P. alni Shahu accessible for genetic engineering, and provided an important reference for developing genetic manipulation methods in other rare actinomycetes.