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BackgroundElevated remnant cholesterol (RC) is associated with a higher risk of various cardiac diseases. Heart failure (HF) usually occurs at the end stage of various cardiac diseases. However, there is limited research on the association between RC and the risk of HF. Therefore, we aimed to provide relevant evidence by determining whether a high RC level also influences the risk of HF.Materials and methodsIn this secondary analysis of the Atherosclerosis Risk in Communities (ARIC) study, we included 12,595 participants without coronary heart disease. We determined the association of the RC level as a continuous or categorical variable with the risk of HF using the multivariable-adjusted Cox proportional hazards models and restricted cubic spline curve.ResultsDuring a median follow-up of 22.5 years, 2,029 (16.1%) cases of HF occurred in all included participants. Compared with participants in the RC < 0.50 mmol/L group, the adjusted hazard ratio (HR) for HF increased progressively in participants with the RC level of 0.50 to 0.99 mmol/L, 1.00 to 1.49 mmol/L, and ≥1.50 mmol/L, from 1.17 (95% confidence interval [CI]: 1.05–1.30) to 1.27(95% CI: 1.08–1.49) and to 1.50 (95% CI: 1.14–1.97) (P for trend < 0.001). Cubic spline curves also revealed that the risk of HF increased with the RC level.ConclusionIn the general population without coronary heart disease, a higher level of RC was significantly associated with a higher risk of HF, indicating that a higher RC level might be a potential risk factor for HF. Therefore, the management of blood cholesterol to reduce the risks of HF should focus not only on the traditional blood lipid parameters but also on the RC level.

Our previous studies have shown that ARA regulated the gonadal steroidogenesis and the synthesis of gonadotrophin-releasing hormone (GnRH) in the brain in a gender-dependent manner in Chinese tongue sole, a marine teleost with typical sex dimorphism. As a following up-study, the current study aimed to clone and characterize a gene responding to dietary ARA differently between males and females, cholesterol 25-hydroxylase (ch25h), which has important roles in cholesterol and lipid metabolism of mammals, but is rarely investigated in fish. The full-length cDNA of Chinese tongue sole ch25h was cloned and characterized, and its transcription in the gonads, brain and liver of both males and females in response to dietary ARA levels (0.34, 2.53, and 9.63% of TFA) was investigated. The Chinese tongue sole Ch25h, which putatively is Ch25h subtype B, shared moderate identity to its known orthologs of other teleost and lower identity to human Ch25h. It has high transcription levels in the gonads, followed by skin and muscle, but low levels in the intestine, spleen, and kidney. High ARA levels significantly increased the ch25h transcription in the gonads and brain of male fish, but did not affect the transcription in the females. However, the effect of dietary ARA on ch25h transcription in the liver showed an opposite gender-difference pattern to those in the gonads and brain. To our knowledge, this is the first study in marine teleost investigating the nutritional regulation of ch25h expression.