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BackgroundGallbladder cancer (GBC) is a malignant cancer with poor prognosis. Evidences have shown that miRNAs are closely related to the occurrence of GBC; thus, we aimed to explore miRNAs, which plays an important role in the occurrence and development of GBC.MethodsMicroarray analysis was performed to investigate the differentially expressed miRNAs between five non-neoplastic gallbladder tissues (normal tissues) and five gallbladder tumor tissues (tumor tissues). RT-qPCR was performed to detect the level of miR-181b-5p in cells, and CCK-8 was performed to detect cell viability. Then, glucose assay kit or lactic acid assay kit was performed to detect the level of glucose consumption or lactate production. Next, transwell and wound healing assays were used to assess cell migration. In addition, dual-luciferase reporter assay was used to verify the relationship between miR-181b-5p and PDHX. At last, Western blotting was performed to determine the protein level of PDHX.ResultsMicroarray analysis suggested miR-181b-5p was significantly upregulated in GBC tumor tissue. KEGG analysis for the protein targets of miR-181b-5p indicates a close relationship existed between miR-181b-5p and glycolysis. In addition, the level of miR-181b-5p was notably increased in GBC-SD or G415 cells, compared with HIBEpiC cells. GBC cell viability was significantly decreased under hypoxia, and these decreases were exacerbated by miR-181b-5p antagomir. Moreover, glucose consumption or lactate production of GBC cells was significantly upregulated under hypoxia, whereas these increases were completely revered by miR-181b-5p antagomir. Further investigation revealed that PDHX was a direct target of miR-181b-5p.ConclusionIn this study, downregulation of miR-181b-5p inhibits the viability, migration, and glycolysis of GBC by upregulating PDHX under hypoxia. This finding suggested that miR-181b-5p might be considered as a novel therapeutic target for the treatment of GBC.

The plateau vortex is one of the dominant weather systems that modulate summer rainfall in northwestern China, a typical arid/semi-arid area worldwide. Two heavy rainfall cases in the summer of 2012 and 2013, accompanying the northeastward movement of plateau vortexes, are selected here. The role of plateau vortexes in these two cases is explored by diagnosing their moist potential vorticity, helicity, and convective clouds characteristics, using numerous data (ERA-Interim reanalysis, multi-sourced precipitation data, FY-2E satellite images). The plateau vortexes enhanced convective instability in the rainfall area by entraining upper-level dry and cold air, manifested as salient vertical gradients of moist potential vorticity and steep pseudo-equivalent potential temperature isolines. The rainfall occurred eastward relative to the vortex movement track and intensified on the left of cloud black body temperature (TBB) with larger gradients at the middle to late stages of the vortex life span. The generation of moisture helicity is dictated by the water vapor transportation, with the negative value concentrated in the water vapor-rich areas. Notably, the two cases differ in tracks, life spans, water vapor sources, and precipitation amounts, which may be attributed to large-scale circulation background, vertical vortex structure, and moisture condition.