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CRISPR/Cas9 is one of the most robust technologies for plant breeding enabling precise and efficient modifications in a genome. This technology is being used for the manipulation of target genes in a host to develop resistance against the plant pathogens. Cucumis sativus elF4E is one of the target genes playing a key role in viral infection during interaction with potyvirus viral proteins genome linked (VPg). Nevertheless, the allelic and positional effect of elF4E mutations in C. sativus is to be clarified in elF4E-VPg interaction. In addition, there are entanglements in the massive production of pathogen-resistant cultivars suitable for commercial production using CRISPR/Cas9 technology. Therefore, we targeted different positions of the elF4E in G27 and G247 inbred lines, using specific gRNA1 and gRNA2 for the first and third exons, respectively, and 1,221 transgene-free plants were selected in segregated T1 generation, where 192 G27 and 79 G247 plants had the least mutation at Cas9 cleavage site of gRNA1 or gRNA2. Crossing was performed to see allelic effects of elfF4E mutations in F1 populations, which were homozygous and heterozygous single (elF4E_1DEL or elF4E_3DEL) and double (elF4E_1-3DEL) mutants. Disease symptoms of watermelon mosaic virus (WMV), papaya ringspot virus (PRSV), and zucchini yellow mosaic virus (ZYMV) were evaluated in both non-edited and edited F1 plants, and we did not observe any symptom in homozygous elF4E_1-3DEL and elF4E_1DEL mutants. However, homozygous elF4E_3DEL was positive in reverse transcription polymerase chain reaction (RT-PCR), even if there were no significant symptoms on the inoculated leaves. ELISA and qRT-PCR indicated lower viral accumulation in homozygous elF4E_3DEL than heterozygous and non-edited plants. Regeneration and transformation protocols were also optimized comprehensively for both the genotypes. The average number of shoots/100 explants was determined for both G27 and G247 as 13.6 and 18.0, respectively. We could not detect any distinguishing difference between the non-edited and edited F1 plants for yield and morphology. Our results demonstrate an effective route for mass production of viral resistant cultivars of cucumber to WMV, ZYMV, and PRSV. In this way, the pathogen-resistant cultivars could be generated to reduce the losses caused by these pathogens in cucumber production.

Robotic burrowing holds promise for applications in agriculture, resource extraction, and infrastructure development, but current approaches are ineffective, inefficient, or cause significant environmental disruption. In contrast, natural burrowers penetrate substrates with minimal disturbance, providing biomechanical principles that could inspire more efficient and sustainable mechanisms. A notable feature of many natural burrowers is their reliance on soft body compositions, raising the question of whether softness contributes to their burrowing success. This review explores the role of soft materials in biological burrowing and their implications for robotic design. We examine the mechanisms that soft-bodied organisms and soft robots employ for submerging and subterranean locomotion, focusing on how softness enhances efficiency and adaptability in granular media. We analyze the gaps between the capabilities of natural burrowers and soft robotic burrowers, identify grand challenges, and propose opportunities to enhance robotic burrowing performance. By bridging biological principles with engineering innovation, this review aims to inform the development of next-generation burrowing robots capable of operating with the efficiency and efficacy seen in nature.

IntroductionMalnutrition is defined as a pathological condition arising from deficient or imbalanced intake of nutritional elements. Factors such as increasing metabolic demands during the disease course in the hospitalized patients and inadequate calorie intake increase the risk of malnutrition. The aim of the present study is to evaluate nutritional status of patients admitted to pediatric intensive care units (PICU) in Turkey, examine the effect of nutrition on the treatment process and draw attention to the need for regulating nutritional support of patients while continuing existing therapies.Material and MethodIn this prospective multicenter study, the data was collected over a period of one month from PICUs participating in the PICU Nutrition Study Group in Turkey. Anthropometric data of the patients, calorie intake, 90-day mortality, need for mechanical ventilation, length of hospital stay and length of stay in intensive care unit were recorded and the relationship between these parameters was examined.ResultsOf the 614 patients included in the study, malnutrition was detected in 45.4% of the patients. Enteral feeding was initiated in 40.6% (n = 249) of the patients at day one upon admission to the intensive care unit. In the first 48 h, 86.82% (n = 533) of the patients achieved the target calorie intake, and 81.65% (n = 307) of the 376 patients remaining in the intensive care unit achieved the target calorie intake at the end of one week. The risk of mortality decreased with increasing upper mid-arm circumference and triceps skin fold thickness Z-score (OR = 0.871/0.894; p = 0.027/0.024). The risk of mortality was 2.723 times higher in patients who did not achieve the target calorie intake at first 48 h (p = 0.006) and the risk was 3.829 times higher in patients who did not achieve the target calorie intake at the end of one week (p = 0.001). The risk of mortality decreased with increasing triceps skin fold thickness Z-score (OR = 0.894; p = 0.024).ConclusionTimely and appropriate nutritional support in critically ill patients favorably affects the clinical course. The results of the present study suggest that mortality rate is higher in patients who fail to achieve the target calorie intake at first 48 h and day seven of admission to the intensive care unit. The risk of mortality decreases with increasing triceps skin fold thickness Z-score.

A distinctive feature of quadrupeds that is integral to their locomotion is the tail. Tails serve many purposes in biological systems, including propulsion, counterbalance, and stabilization while walking, running, climbing, or jumping. Similarly, tails in legged robots may augment the stability and maneuverability of legged robots by providing an additional point of contact with the ground. However, in the field of terrestrial bio-inspired legged robotics, the tail is often ignored because of the difficulties in design and control. In this study, we test the hypothesis that a variable stiffness robotic tail can improve the performance of a sprawling quadruped robot by enhancing its stability and maneuverability in various environments. In order to validate our hypothesis, we integrated a cable-driven, flexible tail with multiple segments into the underactuated sprawling quadruped robot, where a single servo motor working alongside a reel and cable mechanism regulates the tail’s stiffness. Our results demonstrated that by controlling the stiffness of the tail, the stability of locomotion on rough terrain and the climbing ability of the robot are improved compared to the movement with a rigid tail and no tail. Our findings highlight that constant ground support provided by the flexible tail is key to maintaining stable locomotion. This ensured a predictable gait cycle, eliminating unexpected turning and slipping, resulting in an increase in locomotion speed and efficiency. Additionally, we observed the robot’s enhanced climbing ability on surfaces inclined up to 20°. The flexibility of the tail enabled the robot to overcome obstacles without external sensing, exhibiting significant adaptability across various terrains.

The Banff community summoned the TMA Banff Working Group to develop minimum diagnostic criteria (MDC) and recommendations for renal transplant TMA (Tx-TMA) diagnosis, which currently lacks standardized criteria. Using the Delphi method for consensus generation, 23 nephropathologists (panelists) with >3 years of diagnostic experience with Tx-TMA were asked to list light, immunofluorescence, and electron microscopic, clinical and laboratory criteria and differential diagnoses for Tx-TMA. Delphi was modified to include 2 validations rounds with histological evaluation of whole slide images of 37 transplant biopsies (28 TMA and 9 non-TMA). Starting with 338 criteria in R1, MDC were narrowed down to 24 in R8 generating 18 pathological, 2 clinical, 4 laboratory criteria, and 8 differential diagnoses. The panelists reached a good level of agreement (70%) on 76% of the validated cases. For the first time in Banff classification, Delphi was used to reach consensus on MDC for Tx-TMA. Phase I of the study (pathology phase) will be used as a model for Phase II (nephrology phase) for consensus regarding clinical and laboratory criteria. Eventually in Phase III (consensus of the consensus groups) and the final MDC for Tx-TMA will be reported to the transplantation community.

The Thrombotic Microangiopathy Banff Working Group (TMA-BWG) was formed in 2015 to survey current practices and develop minimum diagnostic criteria (MDC) for renal transplant TMA (Tx-TMA). To generate consensus among pathologists and nephrologists, the TMA BWG designed a 3-Phase study. Phase I of the study is presented here. Using the Delphi methodology, 23 panelists with >3 years of diagnostic experience with Tx-TMA pathology listed their MDC suggesting light, immunofluorescence, and electron microscopy lesions, clinical and laboratory information, and differential diagnoses. Nine rounds (R) of consensus resulted in MDC validated during two Rs using online evaluation of whole slide digital images of 37 biopsies (28 TMA, 9 non-TMA). Starting with 338 criteria the process resulted in 24 criteria and 8 differential diagnoses including 18 pathologic, 2 clinical, and 4 laboratory criteria. Results show that 3/4 of the panelists agreed on the diagnosis of 3/4 of cases. The process also allowed definition refinement for 4 light and 4 electron microscopy lesions. For the first time in Banff classification, the Delphi methodology was used to generate consensus. The study shows that Delphi is a democratic and cost-effective method allowing rapid consensus generation among numerous physicians dealing with large number of criteria in transplantation.

IntroductionThere are epistemological studies about the main concept, Crab Syndrome. In this context, the study aims to reveal the antecedents of the Crab Syndrome by evaluating the theoretical background of the Crab Barrel Syndrome within the framework of Social Comparison Theory. The main purpose of this study is to identify the precursors of crab barrel syndrome. In line with this main purpose, the study also aims to reveal the relationship between crab barrel syndrome behaviors and type A versus type B personalities, along with the effect of social comparison behaviors. Type A personality represents the personalities of individuals who are stressed, ambitious, competitive, and quickly take action for their aims. Type B, on the other hand, presents the personality types of individuals who are calm, away from competition, and perform their actions slowly.MethodIt is designed quantitatively, employing scales to quantify type A and type B personalities, social comparison, and crab barrel syndrome. Hypotheses are tested using structural equation modeling.Result/discussionIt is found that there is a positive relation between the type A personality and the crab barrel syndrome, and a negative one between the syndrome and high self-esteem. The findings suggest that by social comparison, type A and type B personality are precursors of crab barrel syndrome. In the study, the theoretical background of the Crab Syndrome was evaluated within the scope of Social Comparison Theory. In this context, examining the relationship between different organizational behavior theories and crab syndrome is important for other studies.

AimsThe aim of this study was to explore the feasibility and benefits of a 12-session dance/movement program for intimate partner violence survivors' mental health and PTSD symptoms during the COVID-19 Pandemic. The specific purposes were fourfold: (1) To determine the feasibility of delivering a virtual dance/movement workshop program; (2) to examine the effects of the program on symptoms of PTSD and psychological distress; (3) to determine whether heart rate variability improves; and (4) to describe the individual and shared experiences of a subgroup of participants of the program.MethodsForty-five women ages 23–48 years were randomized to a 12-session virtual creative dance/movement program or a usual care control group, and completed questionnaires about PTSD and mental health symptoms, general health, physical activity, and underwent a brief measurement of heart rate variability. A subset of the intervention group participated in a semi-structured focus group.ResultsThe results of the study showed that the female survivors of intimate partner violence who participated in the virtual workshops felt better, and they experienced improved affect and reduced tension. They found new ways to express themselves, attune to their bodies, learn new self-care habits, and build community as they engaged in the workshops. Over the course of the study, the participants' symptoms of PTSD and psychological distress lessened. There were no changes in heart rate variability.ConclusionsThis complex study was successfully completed during a global pandemic and resulted in improvements in some mental health symptoms and overall well-being. Given the importance of this work with intimate partner violence survivors, further work exploring dance/movement workshops for participants virtually and in-person is needed.

Numerous worm and arthropod species form physically-connected aggregations in which interactions among individuals give rise to emergent macroscale dynamics and functionalities that enhance collective survival. In particular, some aquatic worms such as the California blackworm (Lumbriculus variegatus) entangle their bodies into dense blobs to shield themselves against external stressors and preserve moisture in dry conditions. Motivated by recent experiments revealing emergent locomotion in blackworm blobs, we investigate the collective worm dynamics by modeling each worm as a self-propelled Brownian polymer. Though our model is two-dimensional, compared to real three-dimensional worm blobs, we demonstrate how a simulated blob can collectively traverse temperature gradients via the coupling between the active motion and the environment. By performing a systematic parameter sweep over the strength of attractive forces between worms, and the magnitude of their directed self-propulsion, we obtain a rich phase diagram which reveals that effective collective locomotion emerges as a result of finely balancing a tradeoff between these two parameters. Our model brings the physics of active filaments into a new meso- and macroscale context and invites further theoretical investigation into the collective behavior of long, slender, semi-flexible organisms.