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Genes are subject to birth and death during the long evolutionary period. Here, young and old duplicate genes were identified in Vernicia fordii. We performed integrative analyses, including expression pattern, gene complexity, evolution, and functional divergence between young and old duplicate genes. Compared with young genes, old genes have higher values of Ka and Ks, lower Ka/Ks values, and lower average intrinsic structural disorder (ISD) values. Gene ontology and RNA-seq suggested that most young and old duplicate genes contained asymmetric functions. Only old duplicate genes are likely to participate in response to Fusarium wilt infection and exhibit divergent expression patterns. Our data suggest that young genes differ from older genes not only by evolutionary properties but also by their function and structure. These results highlighted the characteristics and diversification of the young and old genes in V. fordii and provided a systematic analysis of these genes in the V. fordii genome. [ABSTRACT FROM AUTHOR]

Genes are subject to birth and death during the long evolutionary period. Here, young and old duplicate genes were identified in Vernicia fordii. We performed integrative analyses, including expression pattern, gene complexity, evolution, and functional divergence between young and old duplicate genes. Compared with young genes, old genes have higher values of Ka and Ks, lower Ka/Ks values, and lower average intrinsic structural disorder (ISD) values. Gene ontology and RNA-seq suggested that most young and old duplicate genes contained asymmetric functions. Only old duplicate genes are likely to participate in response to Fusarium wilt infection and exhibit divergent expression patterns. Our data suggest that young genes differ from older genes not only by evolutionary properties but also by their function and structure. These results highlighted the characteristics and diversification of the young and old genes in V. fordii and provided a systematic analysis of these genes in the V. fordii genome.

The tung tree is an important woody oil tree species. Tung oil extracted from the tung fruit seeds is used in the manufacture of environmentally friendly paint. This study investigated the effects of the application of brassinolide (BR) under different temperature conditions on the chlorophyll content, photosynthesis, chlorophyll fluorescence, leaf structure, and chloroplast ultrastructure in Vernicia fordii and Vernicia montana. The conditions used were 8°C-Control (low temperature and no BR), 8°C-BR (low temperature and BR application), 28°C-Control (normal temperature and no BR), and 28°C-BR (normal temperature and BR application), and effects were monitored from 5 to 15 days after the treatments (DAT). The results showed that the low temperature treatment (8°C-Control) significantly reduced the net photosynthetic rate (Pn), stomatal conductance (Gs), maximum fluorescence (Fm), maximum photochemical efficiency (Fv/Fm), and actual photochemical and quantum efficiency (ΦPSII) compared to the control condition (28°C-Control). However, the external application of BR alleviated the negative effects of low-temperature stress to some degree for all the above parameters for both species tested, except for Pn and Gs at 15 DAT. There were no significant differences in most of the parameters in either species between the 28°C-Control and 28°C-BR treatments. At 10 and 15 DAT of low-temperature stress, the 8°C-Control treatment significantly reduced leaf cell tense ratio (CTR) and increased spongy ratio (SR) compared to the 28°C-Control, whereas BR application alleviated the adverse effects. Moreover, the 8°C-Control treatment significantly destroyed the chloroplast structure, loosening the thylakoids until they disintegrated, while exogenous spraying of BR protected the chloroplast structure and enabled it to function properly in both species. Our results suggested that long-term low temperatures significantly reduced the photosynthetic efficiency of tung tree seedlings, affecting the formation of the internal structure of plant leaves and destroying the integrity and function of the chloroplast. To prevent this, external application of BR to tung tree seedlings could enhance the photosynthetic potential of tung trees by maintaining the stability of the leaf structure, morphology, and function, and alleviating the damage caused by cold injury. The results also showed that V. fordii seedlings are more resistant to low temperatures than V. montana seedlings.

The tung tree is an important woody oil tree species. Tung oil extracted from the tung fruit seeds is used in the manufacture of environmentally friendly paint. This study investigated the effects of the application of brassinolide (BR) under different temperature conditions on the chlorophyll content, photosynthesis, chlorophyll fluorescence, leaf structure, and chloroplast ultrastructure in Vernicia fordii and Vernicia montana. The conditions used were 8°C-Control (low temperature and no BR), 8°C-BR (low temperature and BR application), 28°C-Control (normal temperature and no BR), and 28°C-BR (normal temperature and BR application), and effects were monitored from 5 to 15 days after the treatments (DAT). The results showed that the low temperature treatment (8°C-Control) significantly reduced the net photosynthetic rate (Pn), stomatal conductance (Gs), maximum fluorescence (Fm), maximum photochemical efficiency (F v/ F m), and actual photochemical and quantum efficiency (Φ PSII ) compared to the control condition (28 °C-Control). However, the external application of BR alleviated the negative effects of low-temperature stress to some degree for all the above parameters for both species tested, except for P n and G s at 15 DAT. There were no significant differences in most of the parameters in either species between the 28°C-Control and 28°C-BR treatments. At 10 and 15 DAT of low-temperature stress, the 8°C-Control treatment significantly reduced leaf cell tense ratio (CTR) and increased spongy ratio (SR) compared to the 28°C-Control, whereas BR application alleviated the adverse effects. Moreover, the 8°C-Control treatment significantly destroyed the chloroplast structure, loosening the thylakoids until they disintegrated, while exogenous spraying of BR protected the chloroplast structure and enabled it to function properly in both species. Our results suggested that long-term low temperatures significantly reduced the photosynthetic efficiency of tung tree seedlings, affecting the formation of the internal structure of plant leaves and destroying the integrity and function of the chloroplast. To prevent this, external application of BR to tung tree seedlings could enhance the photosynthetic potential of tung trees by maintaining the stability of the leaf structure, morphology, and function, and alleviating the damage caused by cold injury. The results also showed that V. fordii seedlings are more resistant to low temperatures than V. montana seedlings. [ABSTRACT FROM AUTHOR]